Overview

  • Product name
    Anti-PLK1 (phospho T210) antibody [2A3]
    See all PLK1 primary antibodies
  • Description
    Mouse monoclonal [2A3] to PLK1 (phospho T210)
  • Host species
    Mouse
  • Specificity
    ab39068 recognises Plk1 (phospho T210).
  • Tested applications
    Suitable for: ICC/IF, Flow Cyt, WBmore details
  • Species reactivity
    Reacts with: Chicken, Human
  • Immunogen

    Synthetic peptide corresponding to Human PLK1.

  • Positive control
    • Extracts from overnight nocodazole treated Hela cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab39068 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/300.
Flow Cyt Use at an assay dependent concentration.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

WB Use a concentration of 1 µg/ml. Predicted molecular weight: 68 kDa.

Target

  • Function
    Serine/threonine-protein kinase that performs several important functions throughout M phase of the cell cycle, including the regulation of centrosome maturation and spindle assembly, the removal of cohesins from chromosome arms, the inactivation of APC/C inhibitors, and the regulation of mitotic exit and cytokinesis. Required for recovery after DNA damage checkpoint and entry into mitosis. Required for kinetochore localization of BUB1B. Phosphorylates SGOL1. Required for spindle pole localization of isoform 3 of SGOL1 and plays a role in regulating its centriole cohesion function. Phosphorylates BORA, and thereby promotes the degradation of BORA. Contributes to the regulation of AURKA function. Regulates TP53 stability through phosphorylation of TOPORS.
  • Tissue specificity
    Placenta and colon.
  • Sequence similarities
    Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDC5/Polo subfamily.
    Contains 2 POLO box domains.
    Contains 1 protein kinase domain.
  • Developmental stage
    Accumulates to a maximum during the G2 and M phases, declines to a nearly undetectable level following mitosis and throughout G1 phase, and then begins to accumulate again during S phase.
  • Post-translational
    modifications
    Catalytic activity is enhanced by phosphorylation of Thr-210. Phosphorylation at Thr-210 is first detected on centrosomes in the G2 phase of the cell cycle, peaks in prometaphase and gradually disappears from centrosomes during anaphase.
    Autophosphorylation and phosphorylation of Ser-137 may not be significant for the activation of PLK1 during mitosis, but may enhance catalytic activity during recovery after DNA damage checkpoint.
    Ubiquitinated by the anaphase promoting complex/cyclosome (APC/C) in anaphase and following DNA damage, leading to its degradation by the proteasome. Ubiquitination is mediated via its interaction with FZR1/CDH1. Ubiquitination and subsequent degradation prevents entry into mitosis and is essential to maintain an efficient G2 DNA damage checkpoint.
  • Cellular localization
    Nucleus. Chromosome > centromere > kinetochore. Cytoplasm > cytoskeleton > centrosome. During early stages of mitosis, the phosphorylated form is detected on centrosomes and kinetochores. Localizes to the outer kinetochore. Presence of SGOL1 and interaction with the phosphorylated form of BUB1 is required for the kinetochore localization.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cell cycle regulated protein kinase antibody
    • PLK 1 antibody
    • PLK antibody
    • PLK-1 antibody
    • plk1 antibody
    • PLK1_HUMAN antibody
    • Polo like kinase 1 antibody
    • Polo-like kinase 1 antibody
    • Serine/threonine protein kinase 13 antibody
    • Serine/threonine protein kinase PLK1 antibody
    • Serine/threonine-protein kinase 13 antibody
    • Serine/threonine-protein kinase PLK1 antibody
    • STPK 13 antibody
    • STPK13 antibody
    see all

Images

  • All lanes : Anti-PLK1 (phospho T210) antibody [2A3] (ab39068) at 1 µg/ml

    Lane 1 : Extracts from untreated Hela cells
    Lane 2 : Extracts from overnight nocodazole treated Hela cells

    Predicted band size: 68 kDa
    Observed band size: 66 kDa
    why is the actual band size different from the predicted?

  • ab39068 staining chicken B lymphoma cells in prometaphase by ICC/IF. Cells were PFA fixed and permeabilized in 0.15% Triton X-100 prior to blocking in 1% BSA for 1 hour at 4°C. The primary antibody was diluted 1/300 (PBS/1%BSA) and incubated with the sample for 1 hour at 37°C. A Texas Red® conjugated goat polyclonal to mouse, diluted 1/200, was used as the secondary.

    See Abreview

References

This product has been referenced in:
  • Dumitru AMG  et al. Cyclin A/Cdk1 modulates Plk1 activity in prometaphase to regulate kinetochore-microtubule attachment stability. Elife 6:N/A (2017). Read more (PubMed: 29154753) »
  • Naylor RM  et al. Nuclear pore protein NUP88 activates anaphase-promoting complex to promote aneuploidy. J Clin Invest 126:543-59 (2016). Read more (PubMed: 26731471) »
See all 13 Publications for this product

Customer reviews and Q&As

1-10 of 13 Abreviews or Q&A

Answer

Thank you for your inquiry.

While we do not list this as cross reacting with mouse, it is possibnle that it may as the regions are so highly conserved. We generally do not recommend using an antibody created in mouse on mouse tissue because of the high background that may occur.The secondary antibody may recognize endogenous IgG in the sample tissue as well as the primary antibody, which may lead to non-specific staining and high background.

Because we carry over 70,000 products, it isn't feasible for us to keep small sample sizes of our products.

We are happy to reassure our customers that all of our products are covered by our Abpromise, which guarantees that the product will work in the applications and species specified on the datasheet, or we will offer a replacement, credit, or refund within 6 months of purchase.

If the product is to be used in an untested species or application, you may be eligible for our testing discount program if the antibody has not yet been purchased. Please contact our Scientific Support team by replying to this email prior to purchase for more information.

Otherwise, we like to encourage all of our customers to submit an Abreview via the online product datasheet. We always appreciate customer feedback, whether positive or negative, and we make all product information available to researchers. Plus, each Abreview earns Abpoints that can be used for discounts on future purchases or rewards such as Amazon.com gift certificates.

To find out more about our Abreview system, please see the following link:

https://www.abcam.com/abreviews

I hope this information is helpful. Please do not hesitate to contact us again with any other questions.

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Loading amount
50 µg
Gel Running Conditions
Non-reduced Denaturing (10%)
Sample
Human Cell lysate - whole cell (Colon carcinoma)
Specification
Colon carcinoma
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Dr. Eleni Petsalaki

Verified customer

Submitted Dec 04 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa)
Loading amount
100 µg
Specification
HeLa
Gel Running Conditions
Reduced Denaturing (12% gel)
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: RT°C

Ms. AeLee Jeong

Verified customer

Submitted Sep 25 2012

Answer

Thank you for contacting us.
Your credit note ID is xxx.
I am sorry that this antibody did not perform as stated on the datasheet. I have asked our accounting department to issue a credit note for you, which can be redeemed against the invoice of a future order by passing it on to your purchasing department. To avoid confusion, please ensure your accounts department is aware of how the credit note is being used. If you have questions on how to use the credit note, please contact our accounting department.
Our accounting department can be contacted by email at us.credits@abcam.com or by telephone using the information at the Contact Us link in the top right corner of our website. Please refer to the credit note ID in any correspondence with our accounting department.
The credit note ID is for your reference only and does not automatically guarantee the credit.
I hope this experience will not prevent you from purchasing other products from us in the future. Our Scientific Support team is always at your service, should you require further expert advice.

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Answer

Thank you for your reply and the additional information.
I can offer you a free of charge replacement with another antibody of your choice, a credit or refund.
Please let me know what option would be best for you.
I will also get in touch with the supplying lab to let them know your findings and have them re-QC the antibody.
I look forward to hear back from you and assist you further.

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Answer

Thank you for contacting us.
I'm sorry to hear that the antibody does not seem to work as it should.
Could you please let me know the following information in order to understand the situation better:
1) What samples (species, type) are you using?
2) What kind of application are you performing with the antibody (WB, IHC, ICC/IF)?
3) What antibody dilution and incubation time have you used?
4) Is the secondary antibody/detection system working well with other primaries?
5) What is the order or PO number?
I look forward to hear back from you and help you further with this case.
Please do not hesitate to contact us if you need any more advice or information.
Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (Hela)
Loading amount
50 µg
Specification
Hela
Gel Running Conditions
Reduced Denaturing (10)
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Jul 23 2012

Answer

I am very pleased to hear you would like to accept our offer and test ab39068 in MEFs on Western Blot. This code will give you 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, please submit an Abreview for MEFs on Western Blot and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews. Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code. Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research. The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount. While this code is created for testing in WB, I also encourage you to take advantage of our Abreview system when testing this in IF or when testing any of our products antibodies. It takes just a few minutes to leave a review and you can collect Abpoints which you may redeem for products or Amazon gift cards while sharing information about the product with your colleagues worldwide. To leave and Abreview, go to the webpage for the product, click on the Abreview Tab right below the shipping information and click the "Submit an Abreview" link.

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Question
Answer

Thank you very much for your interest in ab39068.

To our knowledge, this has not been tested in Western blot on mouse ebryonic fibroblasts (MEFs). Therefore, I can offer a discount off a future purchase if you buy ab39068 now, test it in WB on MEFs and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of 1 free PRIMARY ANTIBODY.

If you are interested in this offer, please follow these steps:

1. Reply to this e-mail to let me know that you would like to proceed and test ab39068 inWB on MEFs. I will then send a discount code. This code must be issued before purchase so please wait for my reply before ordering.

2. Purchase either by phone, fax, or online (www.abcam.com).

3. Test it in MEFs.

4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any PRIMARY ANTIBODY ordered and the discount code is valid for 4 months after issue.

We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab39068 turns out to be unsuitable , you will still receive the discount on your next purchase after your Abreview has been submitted.

Please let me know if you have any questions about this offer and I would be happy to help you further.

The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

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Answer

Thank you for contacting Abcam.

The immunogen used for this product has 100% homology with the mouse protein (http://www.uniprot.org/uniprot/Q07832). This product has not yet been tested in this species however. Because this has been raised in mouse, using on mouse tissues may lead to high background. As such we cannot guarantee that it would meet the needs of our customers and do not cover this product under our Abpromise guarantee.

We do offer a number of other antibodies against PLK1 (phospho T 210) which have not been raised in mouse. I would suggest ab12157 as an alternative.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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1-10 of 13 Abreviews or Q&A

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