Product nameAnti-PLK1 (phospho T210) antibody
See all PLK1 primary antibodies
DescriptionRabbit polyclonal to PLK1 (phospho T210)
SpecificityDetects a band at 55kDa in mouse A20 cells that we believe corresponds to Plk1 (predicted molecular weight 68kDa). Also gives positive staining in immunohistochemistry in human colon carcinoma as expected. However, we have been unable to conclusively demonstrate the specificity of the antibody for phospho T210 Plk1.
Tested applicationsSuitable for: WB, IHC-P, ELISA, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Xenopus laevis, Drosophila melanogaster, Zebrafish
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride
Concentration information loading...
PurityImmunogen affinity purified
Purification notesPurified by immunoaffinity chromatography using phospho peptide coupled to agarose beads followed by solid phase adsorption(s) against non-phospho peptide and non-specific peptide to remove any unwanted reactivities.
Our Abpromise guarantee covers the use of ab12157 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/200 - 1/2000. Predicted molecular weight: 68 kDa.|
|IHC-P||Use at an assay dependent concentration.|
|ELISA||1/3000 - 1/12000.|
|ICC/IF||Use at an assay dependent concentration.|
FunctionSerine/threonine-protein kinase that performs several important functions throughout M phase of the cell cycle, including the regulation of centrosome maturation and spindle assembly, the removal of cohesins from chromosome arms, the inactivation of APC/C inhibitors, and the regulation of mitotic exit and cytokinesis. Required for recovery after DNA damage checkpoint and entry into mitosis. Required for kinetochore localization of BUB1B. Phosphorylates SGOL1. Required for spindle pole localization of isoform 3 of SGOL1 and plays a role in regulating its centriole cohesion function. Phosphorylates BORA, and thereby promotes the degradation of BORA. Contributes to the regulation of AURKA function. Regulates TP53 stability through phosphorylation of TOPORS.
Tissue specificityPlacenta and colon.
Sequence similaritiesBelongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDC5/Polo subfamily.
Contains 2 POLO box domains.
Contains 1 protein kinase domain.
Developmental stageAccumulates to a maximum during the G2 and M phases, declines to a nearly undetectable level following mitosis and throughout G1 phase, and then begins to accumulate again during S phase.
modificationsCatalytic activity is enhanced by phosphorylation of Thr-210. Phosphorylation at Thr-210 is first detected on centrosomes in the G2 phase of the cell cycle, peaks in prometaphase and gradually disappears from centrosomes during anaphase.
Autophosphorylation and phosphorylation of Ser-137 may not be significant for the activation of PLK1 during mitosis, but may enhance catalytic activity during recovery after DNA damage checkpoint.
Ubiquitinated by the anaphase promoting complex/cyclosome (APC/C) in anaphase and following DNA damage, leading to its degradation by the proteasome. Ubiquitination is mediated via its interaction with FZR1/CDH1. Ubiquitination and subsequent degradation prevents entry into mitosis and is essential to maintain an efficient G2 DNA damage checkpoint.
Cellular localizationNucleus. Chromosome > centromere > kinetochore. Cytoplasm > cytoskeleton > centrosome. During early stages of mitosis, the phosphorylated form is detected on centrosomes and kinetochores. Localizes to the outer kinetochore. Presence of SGOL1 and interaction with the phosphorylated form of BUB1 is required for the kinetochore localization.
- Information by UniProt
- Cell cycle regulated protein kinase antibody
- PLK 1 antibody
- PLK antibody
Anti-PLK1 (phospho T210) antibody (ab12157) at 1/500 dilution + Mouse A20 cell lysate
Predicted band size: 68 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted?
Additional bands at: 23 kDa, 40 kDa, 42 kDa. We are unsure as to the identity of these extra bands.
We believe the 55kDa band represents Plk1. However, we cannot be sure that the antibody is detecting phospho T210 Plk1 specifically.
ab12157 was used at a 1:200 dilution to detect Plk1 by immunohistochemistry in human colon carcinoma tumor tissue. Tissue was formalin-fixed and paraffin embedded. Detection with AEC (pink), nuclear counterstaining with Haematoxylin (blue).
ab12157 (1/200) detecting Plk1 (phospho T210) in formalin-fixed paraffin embedded human breast carcinoma tissue.
This product has been referenced in:
- Oshima K et al. Mutational landscape, clonal evolution patterns, and role of RAS mutations in relapsed acute lymphoblastic leukemia. Proc Natl Acad Sci U S A 113:11306-11311 (2016). Read more (PubMed: 27655895) »
- Li R et al. Involvement of polo-like kinase 1 (Plk1) in mitotic arrest by inhibition of the mitogen-activated protein kinase--extracellular signal-regulated kinase--Ribosomal S6 kinase 1 (MEK-ERK-RSK1) cascade. J Biol Chem : (2012). WB . Read more (PubMed: 22427657) »