Recombinant
RabMAb

Recombinant Anti-PLK1 (phospho T210) antibody [EPNCIR167] - BSA and Azide free (ab240130)

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Overview

  • Product name

    Anti-PLK1 (phospho T210) antibody [EPNCIR167] - BSA and Azide free
    See all PLK1 primary antibodies

  • Description

    Rabbit monoclonal [EPNCIR167] to PLK1 (phospho T210) - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, Dot blot, WBmore details

  • Species reactivity

    Reacts with: Mouse, Human

  • Immunogen

    Synthetic peptide within Human PLK1. The exact sequence is proprietary.

  • General notes

    ab240130 is the carrier-free version of ab155095 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Ab240130 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This antibody was developed as part of a collaboration between the National Cancer Institute's Center for Cancer Research and the lab of Kyung Lee. Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab240130 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.
Dot blot Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 68 kDa.

Target

  • Function

    Serine/threonine-protein kinase that performs several important functions throughout M phase of the cell cycle, including the regulation of centrosome maturation and spindle assembly, the removal of cohesins from chromosome arms, the inactivation of APC/C inhibitors, and the regulation of mitotic exit and cytokinesis. Required for recovery after DNA damage checkpoint and entry into mitosis. Required for kinetochore localization of BUB1B. Phosphorylates SGOL1. Required for spindle pole localization of isoform 3 of SGOL1 and plays a role in regulating its centriole cohesion function. Phosphorylates BORA, and thereby promotes the degradation of BORA. Contributes to the regulation of AURKA function. Regulates TP53 stability through phosphorylation of TOPORS.

  • Tissue specificity

    Placenta and colon.

  • Sequence similarities

    Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDC5/Polo subfamily.
    Contains 2 POLO box domains.
    Contains 1 protein kinase domain.

  • Developmental stage

    Accumulates to a maximum during the G2 and M phases, declines to a nearly undetectable level following mitosis and throughout G1 phase, and then begins to accumulate again during S phase.

  • Post-translational
    modifications

    Catalytic activity is enhanced by phosphorylation of Thr-210. Phosphorylation at Thr-210 is first detected on centrosomes in the G2 phase of the cell cycle, peaks in prometaphase and gradually disappears from centrosomes during anaphase.
    Autophosphorylation and phosphorylation of Ser-137 may not be significant for the activation of PLK1 during mitosis, but may enhance catalytic activity during recovery after DNA damage checkpoint.
    Ubiquitinated by the anaphase promoting complex/cyclosome (APC/C) in anaphase and following DNA damage, leading to its degradation by the proteasome. Ubiquitination is mediated via its interaction with FZR1/CDH1. Ubiquitination and subsequent degradation prevents entry into mitosis and is essential to maintain an efficient G2 DNA damage checkpoint.

  • Cellular localization

    Nucleus. Chromosome > centromere > kinetochore. Cytoplasm > cytoskeleton > centrosome. During early stages of mitosis, the phosphorylated form is detected on centrosomes and kinetochores. Localizes to the outer kinetochore. Presence of SGOL1 and interaction with the phosphorylated form of BUB1 is required for the kinetochore localization.
  • Information by UniProt

  • Database links

    • Alternative names

      • Cell cycle regulated protein kinase antibody
      • PLK 1 antibody
      • PLK antibody
      • PLK-1 antibody
      • plk1 antibody
      • PLK1_HUMAN antibody
      • Polo like kinase 1 antibody
      • Polo-like kinase 1 antibody
      • Serine/threonine protein kinase 13 antibody
      • Serine/threonine protein kinase PLK1 antibody
      • Serine/threonine-protein kinase 13 antibody
      • Serine/threonine-protein kinase PLK1 antibody
      • STPK 13 antibody
      • STPK13 antibody
      see all

    Images

    • Dot Blot - Anti-PLK1 (phospho T210) antibody [EPNCIR167] - BSA and Azide free (ab240130)
      Dot Blot - Anti-PLK1 (phospho T210) antibody [EPNCIR167] - BSA and Azide free (ab240130)

      Anti-PLK1 (phospho T210) antibody [EPNCIR167] (ab155095) Dot Blot. Primary ab dilution 1:1000,  Secondary ab description and code (ab id)Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051), Secondary ab dilution 1:100,000. Blocking buffer and concentration 5% NFDM/TBST, Diluting buffer and concentration 5% NFDM /TBST. Lane 1:PLK1 (pT210) phospho peptide, Lane 2: PLK1 non-phospho peptide, Exposure time 10 seconds. Note: antibody used for ab155095 is purified batch.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab155095).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PLK1 (phospho T210) antibody [EPNCIR167] - BSA and Azide free (ab240130)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PLK1 (phospho T210) antibody [EPNCIR167] - BSA and Azide free (ab240130)

      ab155095 staining PLK1 (phospho T210) in Human colon tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed and paraffin-embedded, antigen retrieval was by heat mediation in Tris/EDTA buffer pH9. Samples were incubated with primary antibody (1/500). An undiluted HRP-conjugated  anti-rabbit IgG was used as the secondary antibody. Tissue counterstained with Hematoxylin. PBS was used in the negative control rather than the Primary antibody.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab155095).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PLK1 (phospho T210) antibody [EPNCIR167] - BSA and Azide free (ab240130)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PLK1 (phospho T210) antibody [EPNCIR167] - BSA and Azide free (ab240130)

      Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling PLK1 with ab155095, unpurified, at 1/100 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab155095).

      Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PLK1 (phospho T210) antibody [EPNCIR167] - BSA and Azide free (ab240130)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PLK1 (phospho T210) antibody [EPNCIR167] - BSA and Azide free (ab240130)

      Immunohistochemical analysis of paraffin-embedded Human gastroic carcinoma tissue labeling PLK1 with ab155095, unpurified, at 1/100 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab155095).

      Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

       

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PLK1 (phospho T210) antibody [EPNCIR167] - BSA and Azide free (ab240130)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PLK1 (phospho T210) antibody [EPNCIR167] - BSA and Azide free (ab240130)

      Immunohistochemical analysis of paraffin embedded Human thyroid gland carcinoma tissue using ab155095, unpurified, showing +ve staining.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab155095).

      Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

       

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PLK1 (phospho T210) antibody [EPNCIR167] - BSA and Azide free (ab240130)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PLK1 (phospho T210) antibody [EPNCIR167] - BSA and Azide free (ab240130)

      Immunohistochemical analysis of paraffin embedded Human cervical carcinoma tissue using ab155095, unpurified, showing +ve staining.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab155095).

      Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

       

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PLK1 (phospho T210) antibody [EPNCIR167] - BSA and Azide free (ab240130)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PLK1 (phospho T210) antibody [EPNCIR167] - BSA and Azide free (ab240130)

      Immunohistochemical analysis of paraffin embedded Human placenta tissue using ab155095, unpurified, showing +ve staining.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab155095).

      Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

       

    References

    ab240130 has not yet been referenced specifically in any publications.

    Publishing research using ab240130? Please let us know so that we can cite the reference in this datasheet.

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