Key features and details
- Rabbit polyclonal to PLK2
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-PLK2 antibody
DescriptionRabbit polyclonal to PLK2
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Mouse, Human
Recombinant fragment, corresponding to a region within amino acids 396-685 of Human PLK2.
- WB: A549 whole cell lysate; IHC-P: Mouse colon tissue.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab137539 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/100 - 1/1000.|
|WB||1/500 - 1/3000. Predicted molecular weight: 78 kDa.|
FunctionMay play a role in the division of at least some cell types, such as fibroblasts, and could function in embryogenesis, wound healing or neoplasia.
Sequence similaritiesBelongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDC5/Polo subfamily.
Contains 2 POLO box domains.
Contains 1 protein kinase domain.
- Information by UniProt
- hPlk2 antibody
- hSNK antibody
- PLK-2 antibody
Anti-PLK2 antibody (ab137539) at 1/1000 dilution + A549 (human lung carcinoma cell line) whole cell lysate at 30 µg
HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 78 kDa
7.5% SDS PAGE
Immunohistochemical analysis paraffin-embedded mouse colon tissue staining PLK2 protein at cytosol with ab137539 at 1/500 dilution.
Antigen Retrieval: EDTA based buffer, pH 8.0, 15min.
ab137539 has been referenced in 2 publications.
- Quan R et al. Proteome Analysis in a Mammalian Cell line Reveals that PLK2 is Involved in Avian Metapneumovirus Type C (aMPV/C)-Induced Apoptosis. Viruses 12:N/A (2020). PubMed: 32231136
- Xie Y et al. Polo-like kinase 2 promotes chemoresistance and predicts limited survival benefit from adjuvant chemotherapy in colorectal cancer. Int J Oncol N/A:N/A (2018). PubMed: 29568868