Anti-PLK3 antibody (ab67922)
Key features and details
- Mouse polyclonal to PLK3
- Suitable for: WB
- Reacts with: Recombinant fragment
- Isotype: IgG
Overview
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Product name
Anti-PLK3 antibody
See all PLK3 primary antibodies -
Description
Mouse polyclonal to PLK3 -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
PLK3 (NP_004064.2, 1 a.a. ~ 646 a.a) full-length human protein.
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Positive control
- PLK3 transfected 293T cell lysate
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.4
Constituent: 2.68% PBS -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab67922 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| WB |
Human
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| Application | Abreviews | Notes |
|---|---|---|
| WB | (1) |
1/500 - 1/1000. Predicted molecular weight: 72 kDa. This antibody has been tested on the recombinant fragment only and we cannot guarantee that it will work on the endogenous protein
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| Notes |
|---|
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WB
1/500 - 1/1000. Predicted molecular weight: 72 kDa. This antibody has been tested on the recombinant fragment only and we cannot guarantee that it will work on the endogenous protein |
Target
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Function
Serine/threonine protein kinase involved in regulating M phase functions during the cell cycle. May also be part of the signaling network controlling cellular adhesion. In vitro, is able to phosphorylate CDC25C and casein. -
Tissue specificity
Transcripts are highly detected in placenta, lung, followed by skeletal muscle, heart, pancreas, ovaries and kidney and weakly detected in liver and brain. May have a short half-live. In cells of hematopoietic origin, strongly and exclusively detected in terminally differentiated macrophages. Transcript expression appears to be down-regulated in primary lung tumor. -
Sequence similarities
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDC5/Polo subfamily.
Contains 2 POLO box domains.
Contains 1 protein kinase domain. -
Post-translational
modificationsPhosphorylated as cells enter mitosis and dephosphorylated as cells exit mitosis. -
Cellular localization
Membrane. - Information by UniProt
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Alternative names
- CNK antibody
- Cytokine Inducible Kinase antibody
- Cytokine-inducible serine/threonine-protein kinase antibody
see all
Images
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Western blot - Anti-PLK3 antibody (ab67922)All lanes : Anti-PLK3 antibody (ab67922) at 1/500 dilution
Lane 1 : PLK3 transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat anti-mouse IgG (H&L)-HRP at 1/2000 dilution
Predicted band size: 72 kDa
Observed band size: 80 kDa why is the actual band size different from the predicted?
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab67922 has been referenced in 1 publication.
- Petrosyan A et al. Restoration of compact Golgi morphology in advanced prostate cancer enhances susceptibility to galectin-1-induced apoptosis by modifying mucin O-glycan synthesis. Mol Cancer Res 12:1704-16 (2014). WB ; Human . PubMed: 25086069