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    plk3-antibody-ab67922.pdf

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Signal Transduction Protein Phosphorylation Ser / Thr Kinases Other Kinases
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Anti-PLK3 antibody (ab67922)

  • Datasheet
  • SDS
Reviews (3) Submit a question References (1)

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Western blot - Anti-PLK3 antibody (ab67922)

    Key features and details

    • Mouse polyclonal to PLK3
    • Suitable for: WB
    • Reacts with: Recombinant fragment
    • Isotype: IgG

    You may also be interested in

    Secondary
    Product image
    Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    Protein
    Recombinant human PLK3 protein (ab42603)

    View more associated products

    Overview

    • Product name

      Anti-PLK3 antibody
      See all PLK3 primary antibodies
    • Description

      Mouse polyclonal to PLK3
    • Host species

      Mouse
    • Tested Applications & Species

      Application Species
      WB
      Human
      See all applications and species data
    • Immunogen

      PLK3 (NP_004064.2, 1 a.a. ~ 646 a.a) full-length human protein.

    • Positive control

      • PLK3 transfected 293T cell lysate
    • General notes

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

      One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

      Learn more here.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
    • Storage buffer

      pH: 7.4
      Constituent: 2.68% PBS
    • Concentration information loading...
    • Purity

      Protein G purified
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Signal Transduction
      • Protein Phosphorylation
      • Ser / Thr Kinases
      • Other Kinases

    Associated products

    • Compatible Secondaries

      • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
      • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    • Isotype control

      • Mouse IgG - Isotype Control (ab37355)
    • Recombinant Protein

      • Recombinant human PLK3 protein (ab42603)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab67922 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Guaranteed

    Tested applications are guaranteed to work and covered by our Abpromise guarantee.

    Predicted

    Predicted to work for this combination of applications and species but not guaranteed.

    Incompatible

    Does not work for this combination of applications and species.

    Application Species
    WB
    Human
    Application Abreviews Notes
    WB (1)
    1/500 - 1/1000. Predicted molecular weight: 72 kDa. This antibody has been tested on the recombinant fragment only and we cannot guarantee that it will work on the endogenous protein
    Notes
    WB
    1/500 - 1/1000. Predicted molecular weight: 72 kDa. This antibody has been tested on the recombinant fragment only and we cannot guarantee that it will work on the endogenous protein

    Target

    • Function

      Serine/threonine protein kinase involved in regulating M phase functions during the cell cycle. May also be part of the signaling network controlling cellular adhesion. In vitro, is able to phosphorylate CDC25C and casein.
    • Tissue specificity

      Transcripts are highly detected in placenta, lung, followed by skeletal muscle, heart, pancreas, ovaries and kidney and weakly detected in liver and brain. May have a short half-live. In cells of hematopoietic origin, strongly and exclusively detected in terminally differentiated macrophages. Transcript expression appears to be down-regulated in primary lung tumor.
    • Sequence similarities

      Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDC5/Polo subfamily.
      Contains 2 POLO box domains.
      Contains 1 protein kinase domain.
    • Post-translational
      modifications

      Phosphorylated as cells enter mitosis and dephosphorylated as cells exit mitosis.
    • Cellular localization

      Membrane.
    • Target information above from: UniProt accession Q9H4B4 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Alternative names

      • CNK antibody
      • Cytokine Inducible Kinase antibody
      • Cytokine-inducible serine/threonine-protein kinase antibody
      • FGF inducible kinase antibody
      • FGF-inducible kinase antibody
      • FNK antibody
      • PLK-3 antibody
      • Plk3 antibody
      • PLK3_HUMAN antibody
      • Polo Like Kinase 3 antibody
      • Polo-like kinase 3 antibody
      • PRK antibody
      • Proliferation Related Kinase antibody
      • Proliferation-related kinase antibody
      • Serine/threonine-protein kinase PLK3 antibody
      see all

    Images

    • Western blot - Anti-PLK3 antibody (ab67922)
      Western blot - Anti-PLK3 antibody (ab67922)
      All lanes : Anti-PLK3 antibody (ab67922) at 1/500 dilution

      Lane 1 : PLK3 transfected 293T cell lysate
      Lane 2 : Non-transfected 293T cell lysate

      Lysates/proteins at 25 µg per lane.

      Secondary
      All lanes : Goat anti-mouse IgG (H&L)-HRP at 1/2000 dilution

      Predicted band size: 72 kDa
      Observed band size: 80 kDa why is the actual band size different from the predicted?

    Protocols

    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (1)

    Publishing research using ab67922? Please let us know so that we can cite the reference in this datasheet.

    ab67922 has been referenced in 1 publication.

    • Petrosyan A  et al. Restoration of compact Golgi morphology in advanced prostate cancer enhances susceptibility to galectin-1-induced apoptosis by modifying mucin O-glycan synthesis. Mol Cancer Res 12:1704-16 (2014). WB ; Human . PubMed: 25086069

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-3 of 3 Abreviews or Q&A

    Immunocytochemistry/ Immunofluorescence abreview for Anti-PLK3 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (Mice Hepatocytes)
    Permeabilization
    Yes - 0.2% Triton X-100 in PBS
    Specification
    Mice Hepatocytes
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
    Fixative
    Formaldehyde
    Read More

    Dr. Armen Petrosyan

    Verified customer

    Submitted Apr 04 2019

    Immunocytochemistry/ Immunofluorescence abreview for Anti-PLK3 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (Panc-1 pancreartic cancer cells)
    Permeabilization
    Yes - 0.2% Triton X-100
    Specification
    Panc-1 pancreartic cancer cells
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Nov 19 2015

    Western blot abreview for Anti-PLK3 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    18 µg
    Gel Running Conditions
    Reduced Denaturing (4-15% gradient SDS-PAGE)
    Sample
    Human Cell lysate - whole cell (HeLa)
    Specification
    HeLa
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More

    Dr. Armen Petrosyan

    Verified customer

    Submitted Nov 18 2014

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