Anti-PLK3 antibody (ab67922)
- Datasheet
- References (1)
- Protocols
Overview
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Product name
Anti-PLK3 antibody
See all PLK3 primary antibodies -
Description
Mouse polyclonal to PLK3 -
Host species
Mouse -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human, Recombinant fragment -
Immunogen
PLK3 (NP_004064.2, 1 a.a. ~ 646 a.a) full-length human protein.
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Positive control
- PLK3 transfected 293T cell lysate
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.20
Constituent: 2.68% PBS -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab67922 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/1000. Predicted molecular weight: 72 kDa. This antibody has been tested on the recombinant fragment only and we cannot guarantee that it will work on the endogenous protein |
Target
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Function
Serine/threonine protein kinase involved in regulating M phase functions during the cell cycle. May also be part of the signaling network controlling cellular adhesion. In vitro, is able to phosphorylate CDC25C and casein. -
Tissue specificity
Transcripts are highly detected in placenta, lung, followed by skeletal muscle, heart, pancreas, ovaries and kidney and weakly detected in liver and brain. May have a short half-live. In cells of hematopoietic origin, strongly and exclusively detected in terminally differentiated macrophages. Transcript expression appears to be down-regulated in primary lung tumor. -
Sequence similarities
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDC5/Polo subfamily.
Contains 2 POLO box domains.
Contains 1 protein kinase domain. -
Post-translational
modificationsPhosphorylated as cells enter mitosis and dephosphorylated as cells exit mitosis. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 1263 Human
- Omim: 602913 Human
- SwissProt: Q9H4B4 Human
- Unigene: 632415 Human
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Alternative names
- CNK antibody
- Cytokine Inducible Kinase antibody
- Cytokine-inducible serine/threonine-protein kinase antibody
see all
Images
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Western blot - Anti-PLK3 antibody (ab67922)This image is courtesy of an Abreview submitted by Armen PetrosyanAnti-PLK3 antibody (ab67922) at 1/2000 dilution + HeLa cell lysate at 18 µg
Secondary
HRP-conjugated donkey anti-mouse IgG polyclonal at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 72 kDa
Observed band size: 95 kDa why is the actual band size different from the predicted?
Exposure time: 45 seconds
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Western blot - Anti-PLK3 antibody (ab67922)All lanes : Anti-PLK3 antibody (ab67922) at 1/500 dilution
Lane 1 : PLK3 transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat anti-mouse IgG (H&L)-HRP at 1/2000 dilution
Predicted band size: 72 kDa
Observed band size: 80 kDa why is the actual band size different from the predicted?
Protocols
Datasheets and documents
References
This product has been referenced in:
- Petrosyan A et al. Restoration of compact Golgi morphology in advanced prostate cancer enhances susceptibility to galectin-1-induced apoptosis by modifying mucin O-glycan synthesis. Mol Cancer Res 12:1704-16 (2014). WB ; Human . Read more (PubMed: 25086069) »
