Product nameAnti-PLK3 antibody
See all PLK3 primary antibodies
DescriptionMouse polyclonal to PLK3
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human, Recombinant fragment
PLK3 (NP_004064.2, 1 a.a. ~ 646 a.a) full-length human protein.
- PLK3 transfected 293T cell lysate
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.20
Constituent: 2.68% PBS
Concentration information loading...
PurityProtein G purified
Our Abpromise guarantee covers the use of ab67922 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Predicted molecular weight: 72 kDa. This antibody has been tested on the recombinant fragment only and we cannot guarantee that it will work on the endogenous protein|
FunctionSerine/threonine protein kinase involved in regulating M phase functions during the cell cycle. May also be part of the signaling network controlling cellular adhesion. In vitro, is able to phosphorylate CDC25C and casein.
Tissue specificityTranscripts are highly detected in placenta, lung, followed by skeletal muscle, heart, pancreas, ovaries and kidney and weakly detected in liver and brain. May have a short half-live. In cells of hematopoietic origin, strongly and exclusively detected in terminally differentiated macrophages. Transcript expression appears to be down-regulated in primary lung tumor.
Sequence similaritiesBelongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDC5/Polo subfamily.
Contains 2 POLO box domains.
Contains 1 protein kinase domain.
modificationsPhosphorylated as cells enter mitosis and dephosphorylated as cells exit mitosis.
- Information by UniProt
- CNK antibody
- Cytokine Inducible Kinase antibody
- Cytokine-inducible serine/threonine-protein kinase antibody
Anti-PLK3 antibody (ab67922) at 1/2000 dilution + HeLa cell lysate at 18 µg
HRP-conjugated donkey anti-mouse IgG polyclonal at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 72 kDa
Observed band size: 95 kDa why is the actual band size different from the predicted?
Exposure time: 45 seconds
All lanes : Anti-PLK3 antibody (ab67922) at 1/500 dilution
Lane 1 : PLK3 transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
All lanes : Goat anti-mouse IgG (H&L)-HRP at 1/2000 dilution
Predicted band size: 72 kDa
Observed band size: 80 kDa why is the actual band size different from the predicted?
This product has been referenced in:
- Petrosyan A et al. Restoration of compact Golgi morphology in advanced prostate cancer enhances susceptibility to galectin-1-induced apoptosis by modifying mucin O-glycan synthesis. Mol Cancer Res 12:1704-16 (2014). WB ; Human . Read more (PubMed: 25086069) »