Product nameAnti-PLK4 antibody
See all PLK4 primary antibodies
DescriptionRabbit polyclonal to PLK4
Tested applicationsSuitable for: WB, ICC/IF, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Cow
Recombinant fragment, corresponding to a region within amino acids 614-871 of Human PLK4 (Uniprot ID O00444).
- 293T, A431, HepG2, Molt-4, Raji, H1299 whole cell lysate, HeLa cells, MCF7 xenograft tissue
Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.025% Proclin
Constituents: PBS, 1% BSA, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab137398 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 109 kDa.|
|ICC/IF||1/100 - 1/1000.|
|IHC-P||1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionSerine/threonine-protein kinase that plays a central role in centriole duplication. Able to trigger procentriole formation on the surface of the parental centriole cylinder, leading to the recruitment of centriole biogenesis proteins such as SASS6, CENPJ/CPAP, CP110, CEP135 and gamma-tubulin. When overexpressed, it is able to induce centrosome amplification through the simultaneous generation of multiple procentrioles adjoining each parental centriole during S phase. Its central role in centriole replication suggests a posible role in tumorigenesis, centrosome aberrations being frequently observed in tumors. Also involved in trophoblast differentiation by phosphorylating HAND1, leading to disrupt the interaction between HAND1 and MDFIC and activate HAND1. Phosphorylates CDC25C and CHEK2/CHK2.
Sequence similaritiesBelongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDC5/Polo subfamily.
Contains 1 POLO box domain.
Contains 1 protein kinase domain.
modificationsUbiquitinated; leading to its degradation by the proteasome.
Tyrosine-phosphorylated by TEC.
Cellular localizationCytoplasm > cytoskeleton > centrosome > centriole. Nucleus > nucleolus. Cleavage furrow. Associates with centrioles throughout the cell cycle. According to PubMed:16244668, it is not present at cleavage furrows.
- Information by UniProt
- PLK 4 antibody
- PLK-4 antibody
- PLK4 antibody
Anti-PLK4 antibody (ab137398) at 1/1000 dilution + H1299 whole cell lysate at 30 µg
Predicted band size: 109 kDa
7.5% SDS PAGE
Immunofluorescence analysis of methanol-fixed HeLa cells labelled with ab137398 at 1/500 dilution (Green). Alpha-tubulin filaments labeled (Red).
Immunohistochemical analysis of paraffin embedded MCF7 xenograft tissue labelled with ab137398 at 1/100 dilution.
This product has been referenced in:
- Wang J et al. Polo-like kinase 4 promotes tumorigenesis and induces resistance to radiotherapy in glioblastoma. Oncol Rep 41:2159-2167 (2019). Read more (PubMed: 30816483) »
- Denu RA et al. Centriole Overduplication is the Predominant Mechanism Leading to Centrosome Amplification in Melanoma. Mol Cancer Res 16:517-527 (2018). Read more (PubMed: 29330283) »