Overview

  • Product name

  • Description

    Rabbit polyclonal to PLK4
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IF, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Cow
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 614-871 of Human PLK4 (Uniprot ID O00444).

  • Positive control

    • 293T, A431, HepG2, Molt-4, Raji, H1299 whole cell lysate, HeLa cells, MCF7 xenograft tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab137398 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 109 kDa.
ICC/IF 1/100 - 1/1000.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Serine/threonine-protein kinase that plays a central role in centriole duplication. Able to trigger procentriole formation on the surface of the parental centriole cylinder, leading to the recruitment of centriole biogenesis proteins such as SASS6, CENPJ/CPAP, CP110, CEP135 and gamma-tubulin. When overexpressed, it is able to induce centrosome amplification through the simultaneous generation of multiple procentrioles adjoining each parental centriole during S phase. Its central role in centriole replication suggests a posible role in tumorigenesis, centrosome aberrations being frequently observed in tumors. Also involved in trophoblast differentiation by phosphorylating HAND1, leading to disrupt the interaction between HAND1 and MDFIC and activate HAND1. Phosphorylates CDC25C and CHEK2/CHK2.
  • Sequence similarities

    Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDC5/Polo subfamily.
    Contains 1 POLO box domain.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications

    Ubiquitinated; leading to its degradation by the proteasome.
    Tyrosine-phosphorylated by TEC.
  • Cellular localization

    Cytoplasm > cytoskeleton > centrosome > centriole. Nucleus > nucleolus. Cleavage furrow. Associates with centrioles throughout the cell cycle. According to PubMed:16244668, it is not present at cleavage furrows.
  • Information by UniProt
  • Database links

  • Alternative names

    • PLK 4 antibody
    • PLK-4 antibody
    • PLK4 antibody
    • PLK4_HUMAN antibody
    • Polo like Kinase 4 antibody
    • Polo-like kinase 4 antibody
    • Protein serine/threonine kinase antibody
    • SAK antibody
    • Serine/Threonine Kinase 18 antibody
    • Serine/threonine kinase antibody
    • Serine/threonine protein kinase 18 antibody
    • Serine/threonine protein kinase PLK4 antibody
    • Serine/threonine protein kinase Sak antibody
    • Serine/threonine-protein kinase 18 antibody
    • Serine/threonine-protein kinase PLK4 antibody
    • Serine/threonine-protein kinase SAK antibody
    • Snk Akin Kinase antibody
    • STK 18 antibody
    • STK18 antibody
    see all

Images

  • Anti-PLK4 antibody (ab137398) at 1/1000 dilution + H1299 whole cell lysate at 30 µg

    Predicted band size: 109 kDa



    7.5% SDS PAGE
  • Immunofluorescence analysis of methanol-fixed HeLa cells labelled with ab137398 at 1/500 dilution (Green). Alpha-tubulin filaments labeled (Red).
  • Immunohistochemical analysis of paraffin embedded MCF7 xenograft tissue labelled with ab137398 at 1/100 dilution.

References

This product has been referenced in:

  • Wang J  et al. Polo-like kinase 4 promotes tumorigenesis and induces resistance to radiotherapy in glioblastoma. Oncol Rep 41:2159-2167 (2019). Read more (PubMed: 30816483) »
  • Denu RA  et al. Centriole Overduplication is the Predominant Mechanism Leading to Centrosome Amplification in Melanoma. Mol Cancer Res 16:517-527 (2018). Read more (PubMed: 29330283) »
See all 5 Publications for this product

Customer reviews and Q&As

Application
Western blot
Loading amount
40 µg
Gel Running Conditions
Non-reduced Denaturing (precast 4-15%)
Sample
Human Cell lysate - whole cell (MCF10A)
Specification
MCF10A
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

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Verified customer

Submitted Aug 19 2014

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