Anti-PLK4 antibody (ab137398)
Key features and details
- Rabbit polyclonal to PLK4
- Suitable for: ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-PLK4 antibody
See all PLK4 primary antibodies -
Description
Rabbit polyclonal to PLK4 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IFmore details
Unsuitable for: IHC-P or WB -
Species reactivity
Reacts with: Human
Predicted to work with: Cow -
Immunogen
Recombinant fragment, corresponding to a region within amino acids 614-871 of Human PLK4 (Uniprot ID O00444).
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Positive control
- ICC/IF: 293T and HeLa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab137398 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
1/100 - 1/1000.
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Notes |
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ICC/IF
1/100 - 1/1000. |
Target
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Function
Serine/threonine-protein kinase that plays a central role in centriole duplication. Able to trigger procentriole formation on the surface of the parental centriole cylinder, leading to the recruitment of centriole biogenesis proteins such as SASS6, CENPJ/CPAP, CP110, CEP135 and gamma-tubulin. When overexpressed, it is able to induce centrosome amplification through the simultaneous generation of multiple procentrioles adjoining each parental centriole during S phase. Its central role in centriole replication suggests a posible role in tumorigenesis, centrosome aberrations being frequently observed in tumors. Also involved in trophoblast differentiation by phosphorylating HAND1, leading to disrupt the interaction between HAND1 and MDFIC and activate HAND1. Phosphorylates CDC25C and CHEK2/CHK2. -
Sequence similarities
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDC5/Polo subfamily.
Contains 1 POLO box domain.
Contains 1 protein kinase domain. -
Post-translational
modificationsUbiquitinated; leading to its degradation by the proteasome.
Tyrosine-phosphorylated by TEC. -
Cellular localization
Cytoplasm > cytoskeleton > centrosome > centriole. Nucleus > nucleolus. Cleavage furrow. Associates with centrioles throughout the cell cycle. According to PubMed:16244668, it is not present at cleavage furrows. - Information by UniProt
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Database links
- Entrez Gene: 10733 Human
- SwissProt: O00444 Human
- Unigene: 172052 Human
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Alternative names
- PLK 4 antibody
- PLK-4 antibody
- PLK4 antibody
see all
Images
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Immunocytochemical analysis of methanol-fixed 233T cells labelled with ab137398 at 1/500 dilution (Green). Alpha-tubulin filaments labeled (Red). Fluoroshield with DAPI (Blue).
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Immunofluorescence analysis of methanol-fixed HeLa cells labelled with ab137398 at 1/500 dilution (Green). Alpha-tubulin filaments labeled (Red).
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (11)
ab137398 has been referenced in 11 publications.
- Singh CK et al. PLK4 is upregulated in prostate cancer and its inhibition reduces centrosome amplification and causes senescence. Prostate 82:957-969 (2022). PubMed: 35333404
- Wang J et al. Inhibition of PLK4 might enhance the anti-tumour effect of bortezomib on glioblastoma via PTEN/PI3K/AKT/mTOR signalling pathway. J Cell Mol Med 24:3931-3947 (2020). PubMed: 32126150
- Denu RA & Burkard ME Analysis of the "centrosome-ome" identifies MCPH1 deletion as a cause of centrosome amplification in human cancer. Sci Rep 10:11921 (2020). PubMed: 32681070
- Meng L et al. A cis-eQTL genetic variant in PLK4 confers high risk of hepatocellular carcinoma. Cancer Med 8:6476-6484 (2019). PubMed: 31489978
- Kim DH et al. Cep131 overexpression promotes centrosome amplification and colon cancer progression by regulating Plk4 stability. Cell Death Dis 10:570 (2019). PubMed: 31358734