Overview

  • Product name

    Anti-PLK4 antibody [OTI3B10]
    See all PLK4 primary antibodies
  • Description

    Mouse monoclonal [OTI3B10] to PLK4
  • Host species

    Mouse
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human PLK4 aa 307-612. Produced in E.coli (NP_055079).
    Database link: O00444

  • Positive control

    • WB: pCMV6-ENTRY PLK4 cDNA transfected HEK-293T cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab236514 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2000. Predicted molecular weight: 109 kDa.

Target

  • Function

    Serine/threonine-protein kinase that plays a central role in centriole duplication. Able to trigger procentriole formation on the surface of the parental centriole cylinder, leading to the recruitment of centriole biogenesis proteins such as SASS6, CENPJ/CPAP, CP110, CEP135 and gamma-tubulin. When overexpressed, it is able to induce centrosome amplification through the simultaneous generation of multiple procentrioles adjoining each parental centriole during S phase. Its central role in centriole replication suggests a posible role in tumorigenesis, centrosome aberrations being frequently observed in tumors. Also involved in trophoblast differentiation by phosphorylating HAND1, leading to disrupt the interaction between HAND1 and MDFIC and activate HAND1. Phosphorylates CDC25C and CHEK2/CHK2.
  • Sequence similarities

    Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDC5/Polo subfamily.
    Contains 1 POLO box domain.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications

    Ubiquitinated; leading to its degradation by the proteasome.
    Tyrosine-phosphorylated by TEC.
  • Cellular localization

    Cytoplasm > cytoskeleton > centrosome > centriole. Nucleus > nucleolus. Cleavage furrow. Associates with centrioles throughout the cell cycle. According to PubMed:16244668, it is not present at cleavage furrows.
  • Information by UniProt
  • Database links

  • Alternative names

    • PLK 4 antibody
    • PLK-4 antibody
    • PLK4 antibody
    • PLK4_HUMAN antibody
    • Polo like Kinase 4 antibody
    • Polo-like kinase 4 antibody
    • Protein serine/threonine kinase antibody
    • SAK antibody
    • Serine/Threonine Kinase 18 antibody
    • Serine/threonine kinase antibody
    • Serine/threonine protein kinase 18 antibody
    • Serine/threonine protein kinase PLK4 antibody
    • Serine/threonine protein kinase Sak antibody
    • Serine/threonine-protein kinase 18 antibody
    • Serine/threonine-protein kinase PLK4 antibody
    • Serine/threonine-protein kinase SAK antibody
    • Snk Akin Kinase antibody
    • STK 18 antibody
    • STK18 antibody
    see all

Images

  • All lanes : Anti-PLK4 antibody [OTI3B10] (ab236514) at 1/2000 dilution

    Lane 1 : pCMV6-ENTRY control cDNA transfected HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
    Lane 2 : pCMV6-ENTRY PLK4 cDNA transfected HEK-293T cell lysate

    Lysates/proteins at 5 µg per lane.

    Predicted band size: 109 kDa

References

ab236514 has not yet been referenced specifically in any publications.

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