Key features and details
- Rabbit polyclonal to PLLP
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-PLLP antibody
See all PLLP primary antibodies
DescriptionRabbit polyclonal to PLLP
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat
- WB: Mouse gonadal, kidney and lung tissue lysate. ICC/IF: HepG2 cells.
This product was previously labelled as Plasmolipin
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Purification notesPurity >95%.
Our Abpromise guarantee covers the use of ab236668 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000.|
|ICC/IF||1/50 - 1/200.|
RelevancePlasmolipin is an 18 kDa membrane-bound proteolipid that belongs to the tetraspan (4TM) family, a rapidly growing group of myelin-associated proteins. Many of these proteins could be linked to human hereditary demyelinating neuropathies. Studies indicate that plasmolipin is a component of the cytoskeletal structure and has been localized to the apical surface of tubular epithelial cells. It has been shown that, upon addition to lipid bilayers, plasmolipin induces formation of ion channels that are both voltage-dependent and potassium-specific. Plasmolipin was originally isolated from kidney tissue. Subsequently, it has been shown to be widely expressed, not only being present in kidney, but also in the nervous system, thymus, testis, lung, and thyroid gland.
Cellular localizationCell Membrane
- Plasma membrane proteolipid (plasmolipin) antibody
- Plasmolipin antibody
- PLLP antibody
HepG2 (human liver hepatocellular carcinoma cell line) cells labeling PLLP (green) using ab236668 at 1/100 dilution in ICC/IF. Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG (H+L) was used as the secondary antibody.
All lanes : Anti-PLLP antibody (ab236668) at 1/1000 dilution
Lane 1 : Mouse gonadal lysate
Lane 2 : Mouse kidney lysate
Lane 3 : Mouse lung lysate
All lanes : Goat polyclonal to rabbit IgG at 1/10000 dilution
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab236668 has not yet been referenced specifically in any publications.