Anti-PLOD3 antibody (ab128698)
Key features and details
- Rabbit polyclonal to PLOD3
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-PLOD3 antibody
See all PLOD3 primary antibodies -
Description
Rabbit polyclonal to PLOD3 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P Human
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Immunogen
Recombinant fragment, corresponding to amino acids 254-508 of Human PLOD3 (BC011674).
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Positive control
- human fetal kidney lysate for western blot, formalin-fixed paraffin-embedded human fetal small intestine for ICH-P
Properties
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Form
Lyophilized:Reconstitute with 200ul distilled sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary. -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituent: 1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab128698 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| IHC-P |
Human
|
| All applications |
Mouse
Rat
|
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P |
1/100 - 1/500.
|
| Notes |
|---|
|
IHC-P
1/100 - 1/500. |
Target
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Function
Forms hydroxylysine residues in -Xaa-Lys-Gly- sequences in collagens. These hydroxylysines serve as sites of attachment for carbohydrate units and are essential for the stability of the intermolecular collagen cross-links. -
Involvement in disease
Defects in PLOD3 are the cause of lysyl hydroxylase 3 deficiency (LH3 deficiency) [MIM:612394]; also known as bone fragility with contractures arterial rupture and deafness. LH3 deficiency is a connective tissue disorder. The syndrome is characterized by congenital malformations severely affecting many tissues and organs and revealing features of several collagen disorders, most of them involving COL2A1 (type II collagen). The findings suggest that the failure of lysyl hydroxylation and hydroxylysyl carbohydrate addition, which affects many collagens, is the molecular basis of this syndrome. -
Sequence similarities
Contains 1 Fe2OG dioxygenase domain. -
Cellular localization
Rough endoplasmic reticulum membrane. - Information by UniProt
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Database links
- Entrez Gene: 8985 Human
- Entrez Gene: 26433 Mouse
- Entrez Gene: 288583 Rat
- Omim: 603066 Human
- SwissProt: O60568 Human
- SwissProt: Q9R0E1 Mouse
- SwissProt: Q5U367 Rat
- Unigene: 153357 Human
see all -
Alternative names
- 2-oxoglutarate 5-dioxygenase 3 antibody
- bone protein I antibody
- BP-I antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PLOD3 antibody (ab128698)ab128698, at a dilution of 1/100, staining PLOD3 in Formalin-fixed, Paraffin-embedded human fetal small intestine by Immunohistochemistry
References (2)
ab128698 has been referenced in 2 publications.
- Li H et al. Overexpression of LH3 reduces the incidence of hypertensive intracerebral hemorrhage in mice. J Cereb Blood Flow Metab 39:547-561 (2019). PubMed: 30516406
- Shao S et al. Lysyl Hydroxylase Inhibition by Minoxidil Blocks Collagen Deposition and Prevents Pulmonary Fibrosis via TGF-ß1/Smad3 Signaling Pathway. Med Sci Monit 24:8592-8601 (2018). PubMed: 30481795
