Key features and details
- Mouse polyclonal to PLOD3
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-PLOD3 antibody
See all PLOD3 primary antibodies
DescriptionMouse polyclonal to PLOD3
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Human
Full length Human PLOD3 (NP_001075.1)
- HepG2 cell lysate, PLOD3 transfected 293T cell lysate and HeLa cell
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.4
Constituent: 2.68% PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab89263 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use at a concentration of 1 - 5 µg/ml. Predicted molecular weight: 85 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionForms hydroxylysine residues in -Xaa-Lys-Gly- sequences in collagens. These hydroxylysines serve as sites of attachment for carbohydrate units and are essential for the stability of the intermolecular collagen cross-links.
Involvement in diseaseDefects in PLOD3 are the cause of lysyl hydroxylase 3 deficiency (LH3 deficiency) [MIM:612394]; also known as bone fragility with contractures arterial rupture and deafness. LH3 deficiency is a connective tissue disorder. The syndrome is characterized by congenital malformations severely affecting many tissues and organs and revealing features of several collagen disorders, most of them involving COL2A1 (type II collagen). The findings suggest that the failure of lysyl hydroxylation and hydroxylysyl carbohydrate addition, which affects many collagens, is the molecular basis of this syndrome.
Sequence similaritiesContains 1 Fe2OG dioxygenase domain.
Cellular localizationRough endoplasmic reticulum membrane.
- Information by UniProt
- 2-oxoglutarate 5-dioxygenase 3 antibody
- bone protein I antibody
- BP-I antibody
Anti-PLOD3 antibody (ab89263) at 5 µg/ml + HepG2 cel lysate at 50 µg
Predicted band size: 85 kDa
All lanes : Anti-PLOD3 antibody (ab89263) at 5 µg/ml
Lane 1 : PLOD3 transfected 293T cell lysate
Lane 2 : Non-transfected lysate
Lysates/proteins at 50 µg per lane.
Predicted band size: 85 kDa
ab89263 at 10µg/ml staining PLOD3 on HeLa cell by immunofluorescence
ab89263 has been referenced in 1 publication.
- Shao S et al. Lysyl Hydroxylase Inhibition by Minoxidil Blocks Collagen Deposition and Prevents Pulmonary Fibrosis via TGF-ß1/Smad3 Signaling Pathway. Med Sci Monit 24:8592-8601 (2018). PubMed: 30481795