Anti-PLOD3 antibody (ab89263)
Key features and details
- Mouse polyclonal to PLOD3
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-PLOD3 antibody
See all PLOD3 primary antibodies -
Description
Mouse polyclonal to PLOD3 -
Host species
Mouse -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein within Human PLOD3. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
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Positive control
- HepG2 cell lysate, PLOD3 transfected 293T cell lysate and HeLa cell
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.40
Constituent: 100% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab89263 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 85 kDa.
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ICC/IF | (1) |
Use a concentration of 10 µg/ml.
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Notes |
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WB
Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 85 kDa. |
ICC/IF
Use a concentration of 10 µg/ml. |
Target
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Function
Forms hydroxylysine residues in -Xaa-Lys-Gly- sequences in collagens. These hydroxylysines serve as sites of attachment for carbohydrate units and are essential for the stability of the intermolecular collagen cross-links. -
Involvement in disease
Defects in PLOD3 are the cause of lysyl hydroxylase 3 deficiency (LH3 deficiency) [MIM:612394]; also known as bone fragility with contractures arterial rupture and deafness. LH3 deficiency is a connective tissue disorder. The syndrome is characterized by congenital malformations severely affecting many tissues and organs and revealing features of several collagen disorders, most of them involving COL2A1 (type II collagen). The findings suggest that the failure of lysyl hydroxylation and hydroxylysyl carbohydrate addition, which affects many collagens, is the molecular basis of this syndrome. -
Sequence similarities
Contains 1 Fe2OG dioxygenase domain. -
Cellular localization
Rough endoplasmic reticulum membrane. - Information by UniProt
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Database links
- Entrez Gene: 8985 Human
- Omim: 603066 Human
- SwissProt: O60568 Human
- Unigene: 153357 Human
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Alternative names
- 2-oxoglutarate 5-dioxygenase 3 antibody
- bone protein I antibody
- BP-I antibody
see all
Images
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Anti-PLOD3 antibody (ab89263) at 5 µg/ml + HepG2 cel lysate at 50 µg
Predicted band size: 85 kDa -
All lanes : Anti-PLOD3 antibody (ab89263) at 5 µg/ml
Lane 1 : PLOD3 transfected 293T cell lysate
Lane 2 : Non-transfected lysate
Lysates/proteins at 50 µg per lane.
Predicted band size: 85 kDa -
ab89263 at 10µg/ml staining PLOD3 on HeLa cell by immunofluorescence
Protocols
Datasheets and documents
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Datasheet download
References (3)
ab89263 has been referenced in 3 publications.
- Chen Y et al. PLOD3 contributes to HER-2 therapy resistance in gastric cancer through FoxO3/Survivin pathway. Cell Death Discov 8:321 (2022). PubMed: 35835735
- Shao S et al. Lysyl Hydroxylase Inhibition by Minoxidil Blocks Collagen Deposition and Prevents Pulmonary Fibrosis via TGF-ß1/Smad3 Signaling Pathway. Med Sci Monit 24:8592-8601 (2018). PubMed: 30481795
- Lin Q et al. Analysis of colorectal cancer glyco-secretome identifies laminin β-1 (LAMB1) as a potential serological biomarker for colorectal cancer. Proteomics 15:3905-20 (2015). PubMed: 26359947