Overview

  • Product name

    Anti-PLP2 antibody [EPR14238(B)]
    See all PLP2 primary antibodies
  • Description

    Rabbit monoclonal [EPR14238(B)] to PLP2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt, IP, WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human PLP2 aa 100 to the C-terminus. The exact sequence is proprietary.
    Database link: Q04941

  • Positive control

    • Human colon cancer and cerebellum lysates; SW480 cells; U87-MG cells.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab180131 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/80.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

IP 1/30.
WB 1/2000 - 1/5000. Predicted molecular weight: 17 kDa.
ICC/IF 1/250.

Target

  • Function

    May play a role in cell differentiation in the intestinal epithelium.
  • Tissue specificity

    Enriched in colonic mucosa. The expression of A4 follows a gradient along the crypto-villus axis with the most abundant message occurring in the lower half of the crypt.
  • Sequence similarities

    Contains 1 MARVEL domain.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • A4 antibody
    • A4 differentiation dependent protein antibody
    • A4-LSB antibody
    • A4LSB antibody
    • differentiation dependent protein A4 antibody
    • Differentiation-dependent protein A4 antibody
    • Intestinal membrane A4 protein antibody
    • MGC126187 antibody
    • PLP2 antibody
    • PLP2_HUMAN antibody
    • proteolipid protein 2 (colonic epithelium enriched) antibody
    • Proteolipid protein 2 antibody
    see all

Images

  • All lanes : Anti-PLP2 antibody [EPR14238(B)] (ab180131) at 1/5000 dilution

    Lane 1 : Human colon cancer lysate
    Lane 2 : Human cerebellum lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab136636) at 1/500 dilution

    Predicted band size: 17 kDa

  • Immunofluorescence analysis of SW480 cells labeling PLP2 using ab180131 at 1/250 dilution. A Goat anti rabbit IgG (Dylight 488) at 1/250 dilution was used as secondary antibody. Cells were fixed with 4% paraformaldehyde. Counterstain: DAPI.

  • Immunoprecipitation analysis of Human colon cancer lysate labeling PLP2 using ab180131 at 1/30 dilution. A Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 was used as secondary antibody.

  • Flow cytometry analysis of U87-MG cells labeling PLP2 using ab180131 at 1/80 dilution. A Goat anti rabbit IgG (FITC) at 1/75 dilution was used as secondary antibody. Cells were fixed with 2% paraformaldehyde. Isotype control: Rabbit IgG.

References

This product has been referenced in:

  • Chen YH  et al. Proteolipid Protein 2 Overexpression Indicates Aggressive Tumor Behavior and Adverse Prognosis in Human Gliomas. Int J Mol Sci 19:N/A (2018). Read more (PubMed: 30373180) »
  • Ding Z  et al. Loss of MiR-664 Expression Enhances Cutaneous Malignant Melanoma Proliferation by Upregulating PLP2. Medicine (Baltimore) 94:e1327 (2015). WB . Read more (PubMed: 26287415) »
See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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