Recombinant
RabMAb

Recombinant Anti-PLP2 antibody [EPR14238(B)] (Alexa Fluor® 488) (ab208266)

Overview

  • Product name

    Anti-PLP2 antibody [EPR14238(B)] (Alexa Fluor® 488)
    See all PLP2 primary antibodies
  • Description

    Rabbit monoclonal [EPR14238(B)] to PLP2 (Alexa Fluor® 488)
  • Host species

    Rabbit
  • Conjugation

    Alexa Fluor® 488. Ex: 495nm, Em: 519nm
  • Tested applications

    Suitable for: ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human PLP2 aa 100 to the C-terminus. The exact sequence is proprietary.
    Database link: Q04941

  • Positive control

    • ICC/IF: SW480 cells
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab208266 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100.

This product gave a positive signal in SW480 cells fixed with 4% formaldehyde (10 min) and 100% methanol (5 min)

Target

  • Function

    May play a role in cell differentiation in the intestinal epithelium.
  • Tissue specificity

    Enriched in colonic mucosa. The expression of A4 follows a gradient along the crypto-villus axis with the most abundant message occurring in the lower half of the crypt.
  • Sequence similarities

    Contains 1 MARVEL domain.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • A4 antibody
    • A4 differentiation dependent protein antibody
    • A4-LSB antibody
    • A4LSB antibody
    • differentiation dependent protein A4 antibody
    • Differentiation-dependent protein A4 antibody
    • Intestinal membrane A4 protein antibody
    • MGC126187 antibody
    • PLP2 antibody
    • PLP2_HUMAN antibody
    • proteolipid protein 2 (colonic epithelium enriched) antibody
    • Proteolipid protein 2 antibody
    see all

Images

  • ab208266 staining PLP2 in SW480 cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab208266 at a 1/100 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at a 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    This product also gave a positive signal under the same testing conditions in SW480 cells fixed with 100% methanol (5 min)

References

ab208266 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab208266.
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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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