Key features and details
- Rabbit polyclonal to PLRG1
- Suitable for: WB, IP, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-PLRG1 antibody
See all PLRG1 primary antibodies
DescriptionRabbit polyclonal to PLRG1
Tested applicationsSuitable for: WB, IP, IHC-Pmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Xenopus laevis, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis, Platypus
Synthetic peptide corresponding to a region between residues 464 and 514 of Human PLRG1 (NP_002660.1).
- HeLa, 293T and NIH3T3 whole cell lysates.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab86050 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Detects a band of approximately 57 kDa (predicted molecular weight: 57 kDa).|
|IP||Use at 2-5 µg/mg of lysate.|
|IHC-P||1/200 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionNecessary for spliceosome assembly and for pre-mRNA splicing.
Sequence similaritiesBelongs to the WD repeat PRL1/PRL2 family.
Contains 7 WD repeats.
Cellular localizationNucleus speckle.
- Information by UniProt
- Cwc1 antibody
- MGC110980 antibody
- pleiotropic regulator 1 (PRL1 homolog, Arabidopsis) antibody
All lanes : Anti-PLRG1 antibody (ab86050) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Lane 5 : NIH3T3 whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 57 kDa
Observed band size: 57 kDa
Additional bands at: 45 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 3 seconds
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma (left) and breast carcinoma (right) tissues labelling PLRG1 with ab86050 at 1/200 (1µg/ml). Detection: DAB and a DyLight® 594-conjugated goat anti-rabbit IgG (H+L) (1/100).
Detection of PLRG1 in Immunoprecipitates of HeLa whole cell lysate (1 mg for IP, 20% of IP loaded) using ab86050 at 3 µg/mg lysate for IP and at 0.4 µg/ml for subsequent Western blot detection. Detection: Chemiluminescence with exposure time of 3 seconds.
ab86050 has been referenced in 1 publication.
- van Maldegem F et al. CTNNBL1 facilitates the association of CWC15 with CDC5L and is required to maintain the abundance of the Prp19 spliceosomal complex. Nucleic Acids Res 43:7058-69 (2015). WB ; Mouse . PubMed: 26130721