Anti-PLRG1 antibody (ab86050)
Key features and details
- Rabbit polyclonal to PLRG1
- Suitable for: WB, IP, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-PLRG1 antibody -
Description
Rabbit polyclonal to PLRG1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Cow -
Immunogen
Synthetic peptide within Human PLRG1 aa 450 to the C-terminus. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: O43660 -
Positive control
- WB: HeLa, HEK-293T and NIH/3T3 whole cell lysates. IHC-P: Human breast carcinoma and prostate carcinoma tissue. IP: HeLa whole cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab86050 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/2000 - 1/10000. Detects a band of approximately 57 kDa (predicted molecular weight: 57 kDa).
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IP |
Use at 2-5 µg/mg of lysate.
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IHC-P |
1/200 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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WB
1/2000 - 1/10000. Detects a band of approximately 57 kDa (predicted molecular weight: 57 kDa). |
IP
Use at 2-5 µg/mg of lysate. |
IHC-P
1/200 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Necessary for spliceosome assembly and for pre-mRNA splicing. -
Sequence similarities
Belongs to the WD repeat PRL1/PRL2 family.
Contains 7 WD repeats. -
Cellular localization
Nucleus speckle. - Information by UniProt
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Database links
- Entrez Gene: 533951 Cow
- Entrez Gene: 5356 Human
- Entrez Gene: 53317 Mouse
- Entrez Gene: 60376 Rat
- Omim: 605961 Human
- SwissProt: Q2KID6 Cow
- SwissProt: O43660 Human
- SwissProt: Q922V4 Mouse
see all -
Alternative names
- Cwc1 antibody
- MGC110980 antibody
- pleiotropic regulator 1 (PRL1 homolog, Arabidopsis) antibody
see all
Images
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All lanes : Anti-PLRG1 antibody (ab86050) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Lane 5 : NIH3T3 whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 57 kDa
Observed band size: 57 kDa
Additional bands at: 45 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 3 seconds -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma (left) and breast carcinoma (right) tissues labelling PLRG1 with ab86050 at 1/200 (1µg/ml). Detection: DAB and a DyLight® 594-conjugated goat anti-rabbit IgG (H+L) (1/100).
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Detection of PLRG1 in Immunoprecipitates of HeLa whole cell lysate (1 mg for IP, 20% of IP loaded) using ab86050 at 3 µg/mg lysate for IP and at 0.4 µg/ml for subsequent Western blot detection. Detection: Chemiluminescence with exposure time of 3 seconds.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab86050 has been referenced in 1 publication.
- van Maldegem F et al. CTNNBL1 facilitates the association of CWC15 with CDC5L and is required to maintain the abundance of the Prp19 spliceosomal complex. Nucleic Acids Res 43:7058-69 (2015). WB ; Mouse . PubMed: 26130721