Overview

  • Product name

    PLTP Activity Assay Kit II (Fluorometric)
  • Detection method

    Fluorescent
  • Sample type

    Serum, Plasma, Purified protein
  • Assay type

    Enzyme activity
  • Species reactivity

    Reacts with: Other species, Mammals
  • Product overview

    PLTP Activity Assay Kit II (Fluorometric) (ab196998) uses a donor molecule containing a fluorescent self-quenched phospholipid that is transferred to an acceptor molecule in the presence of Phospholipid Transfer Protein (PLTP). PLTP-mediated transfer of the fluorescent phospholipid to the acceptor molecule results in an increase in fluorescence (Ex/Em = 465/535 nm). The fluorometric intensity is directly proportional to the amount of phospholipid transferred. The kit contains rabbit serum as positive control for assay validation.


    This assay can measure PLTP activity in serum but can also be used for testing activity of recombinant PLTP protein.

  • Notes

    Phospholipid Transfer Protein (PLTP) is a protein found in mammalian plasma that plays an important role in transferring phospholipids between high-density lipoprotein (HDL) molecules and in the modulation of HDL size and composition, as well as in controlling plasma HDL levels. PLTP also plays a key role in the uptake of cholesterol from peripheral cells and tissues that is subsequently transported to the liver for degradation and excretion. Two distinct forms of PLTP exist in plasma: an active form that can transfer PC from phospholipid vesicles to high-density lipoproteins (HDL), and an inactive form that lacks this capability. 

    Due to its role in cholesterol transport, PLTP maybe promote atherosclerosis and is, therefore, considered a promising target for pharmacological intervention.

     

  • Platform

    Microplate reader

Properties

Images

  • Donor Molecule Standard Curve

  • Measurement of PLTP activity of rabbit serum (1 µL), Human plasma (1 µL).

Protocols

References

ab196998 has not yet been referenced specifically in any publications.

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