Product namePLTP Activity Assay Kit II (Fluorometric)
Sample typeSerum, Plasma, Purified protein
Assay typeEnzyme activity
Species reactivityReacts with: Other species, Mammals
PLTP Activity Assay Kit II (Fluorometric) (ab196998) uses a donor molecule containing a fluorescent self-quenched phospholipid that is transferred to an acceptor molecule in the presence of Phospholipid Transfer Protein (PLTP). PLTP-mediated transfer of the fluorescent phospholipid to the acceptor molecule results in an increase in fluorescence (Ex/Em = 465/535 nm). The fluorometric intensity is directly proportional to the amount of phospholipid transferred. The kit contains rabbit serum as positive control for assay validation.
This assay can measure PLTP activity in serum but can also be used for testing activity of recombinant PLTP protein.
Phospholipid Transfer Protein (PLTP) is a protein found in mammalian plasma that plays an important role in transferring phospholipids between high-density lipoprotein (HDL) molecules and in the modulation of HDL size and composition, as well as in controlling plasma HDL levels. PLTP also plays a key role in the uptake of cholesterol from peripheral cells and tissues that is subsequently transported to the liver for degradation and excretion. Two distinct forms of PLTP exist in plasma: an active form that can transfer PC from phospholipid vesicles to high-density lipoproteins (HDL), and an inactive form that lacks this capability.
Due to its role in cholesterol transport, PLTP maybe promote atherosclerosis and is, therefore, considered a promising target for pharmacological intervention.
Storage instructionsPlease refer to protocols.
Components 100 tests Donor Molecule 1 x 0.2ml PLTP Acceptor Molecule 1 x 0.5ml PLTP Assay Buffer 1 x 20ml Positive Control 1 x 0.1ml
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipid metabolism
RelevancePlasma phospholipid transfer protein (PLTP) is thought to play a major role in the facilitated transfer of phospholipids between lipoproteins and in the modulation of high-density lipoprotein (HDL) particle size and composition. PLTP-facilitated lipid transfer activity is related to HDL and LDL metabolism, as well as lipoprotein lipase activity, adiposity, and insulin resistance.
- Lipid transfer protein II
- Phospholipid transfer protein
ab196998 has not yet been referenced specifically in any publications.