Recombinant
RabMAb

Recombinant Anti-Plunc antibody [EPR8381] (ab131163)

Overview

  • Product name

    Anti-Plunc antibody [EPR8381]
    See all Plunc primary antibodies
  • Description

    Rabbit monoclonal [EPR8381] to Plunc
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, Flow Cytmore details
    Unsuitable for: ICC or IHC-P
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Plunc aa 150-250. The exact sequence is proprietary.

  • Positive control

    • A549, PC3, Jurkat, 293T, Human lung squamous cell cancer and Human fetal lung lysates; Jurkat cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Dissociation constant (KD)

    KD = 3.90 x 10 -12 M
    Learn more about KD
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR8381
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab131163 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 25 kDa (predicted molecular weight: 27 kDa).
IP 1/10 - 1/100.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for ICC or IHC-P.
  • Target

    • Relevance

      May be involved in the airway inflammatory response after exposure to irritants. May be associated with tumor progression. May play a role in innate immune responses of the upper airways.
    • Cellular localization

      Secreted. Note: Found in the nasal mucus. Apical side of airway epithelial cells. Detected in nasal mucus
    • Database links

    • Alternative names

      • BPIFA1 antibody
      • bA49G10.5 antibody
      • LPLUNC3 antibody
      • Lung-specific protein X antibody
      • LUNX antibody
      • NASG antibody
      • Nasopharyngeal carcinoma-related protein antibody
      • Palate lung and nasal epithelium clone protein antibody
      • PLUNC antibody
      • Protein Plunc antibody
      • Secretory protein in upper respiratory tracts antibody
      • SPLUNC1 antibody
      • SPURT antibody
      • Tracheal epithelium-enriched protein antibody
      • Von Ebner protein Hl antibody
      see all

    Images

    • All lanes : Anti-Plunc antibody [EPR8381] (ab131163) at 1/1000 dilution

      Lane 1 : A549 cell lysate
      Lane 2 : PC3 cell lysate
      Lane 3 : Human lung squamous cell cancer lysate
      Lane 4 : Human fetal lung lysate
      Lane 5 : Jurkat cell lysate
      Lane 6 : 293T cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit IgG at 1/2000 dilution

      Predicted band size: 27 kDa
      Observed band size: 25 kDa
      why is the actual band size different from the predicted?

    • Flow cytometric analysis of Jurkat cells labelling Plunc (red) or a rabbit IgG negative control (green) with ab131163 antibody at 1/10 dilution.
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    ab131163 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab131163.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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