(±)-Blebbistatin, inhibitor of myosin II (ab120425)

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Functional Studies - (±)-Blebbistatin, inhibitor of myosin II (ab120425)

    Key features and details

    • Potent, selective, inhibitor of myosin II
    • CAS Number: 674289-55-5
    • Purity: > 99%
    • Soluble in DMSO to 100 mM
    • Form / State: Solid
    • Source: Synthetic

    Overview

    • Product name

      (±)-Blebbistatin, inhibitor of myosin II

    • Description

      Potent, selective, inhibitor of myosin II

    • Biological description

      Cell-permeable, reversible inhibitor of non-muscle myosin II. Shows high affinity and selectivity for vertebrate and invertebrate Myosin II (IC50 values 0.5-5 μM). Inhibits actin-activated MgATPase activity and in vitro motility of class II myosins. Also inhibits contraction of the cytokinetic ring. Blocks cell blebbing and disrupts directed cell migration and cytokinesis.

    • Purity

      > 99%

    • CAS Number

      674289-55-5

    • Chemical structure

      Chemical Structure

    Properties

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab120425 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Functional Studies
    Use at an assay dependent concentration.
    Notes
    Functional Studies
    Use at an assay dependent concentration.

    Images

    • Functional Studies - (±)-Blebbistatin, inhibitor of myosin II (ab120425)
      Functional Studies - (±)-Blebbistatin, inhibitor of myosin II (ab120425)
      ab32084 staining paxillin in MEF1 cells treated with (+/-)-blebbistatin (ab120425), by ICC/IF. Decreased membrane expression of paxillin correlates with increased concentration of (+/-)-blebbistatin, as described in literature.
      The cells were incubated at 37°C for 1h in media containing different concentrations of ab120425 ((+/-)-blebbistatin) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab32084 (1/100 dilution) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (16)

    Publishing research using ab120425? Please let us know so that we can cite the reference in this datasheet.

    ab120425 has been referenced in 16 publications.

    • Wu N  et al. Critical Role of Lipid Scramblase TMEM16F in Phosphatidylserine Exposure and Repair of Plasma Membrane after Pore Formation. Cell Rep 30:1129-1140.e5 (2020). PubMed: 31995754
    • Stanton AE  et al. Biochemical Ligand Density Regulates Yes-Associated Protein Translocation in Stem Cells through Cytoskeletal Tension and Integrins. ACS Appl Mater Interfaces 11:8849-8857 (2019). PubMed: 30789697
    • Zhovmer AS  et al. The role of nonmuscle myosin 2A and 2B in the regulation of mesenchymal cell contact guidance. Mol Biol Cell 30:1961-1973 (2019). PubMed: 31318315
    • Hu X  et al. Release of cholesterol-rich particles from the macrophage plasma membrane during movement of filopodia and lamellipodia. Elife 8:N/A (2019). PubMed: 31486771
    • Simeonov S & Schäffer TE Ultrafast Imaging of Cardiomyocyte Contractions by Combining Scanning Ion Conductance Microscopy with a Microelectrode Array. Anal Chem 91:9648-9655 (2019). PubMed: 31247725
    View all Publications for this product

    Customer reviews and Q&As

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    1-2 of 2 Abreviews or Q&A

    Question

    I have reconstituted ab120425 as directed in DMSO and stored in aliquots at -20 deg C; I am thawing aliquots and adding to tissue culture growth medium for cell treatments; first 2 weeks things seemed fine but recently adding thawed aliquots of compound to growth medium seems to produce aggregates; not sure if it is solubility issue in medium or due to precipitation in product itself; any ideas what is causing the aggregates?

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    Answer



    It sounds like there is some precipitation because the product hasn’t equilibrated to room temperature fully before use. Precipitation can occur easily when thawing frozen DMSO solutions and the aliquots need to be inspected carefully before they are used.


    These are our guidelines for storing and using solutions at -20°C:

    Wherever possible, prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
    Solubility is temperature dependent. As such, cooling or freezing solutions may lead to precipitation of the product from solution. It is therefore important to ensure that your product is completely re-dissolved before use.

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    Question

    Product code: 120425
    Inquiry: Hi Could you please tell me if this product in supplied in solution? Thanks

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    Answer

    Thank you for your enquiry and your interest in our products.

    I can confirm that this product (ab120425: Blebbistatin) is in solid form and we sell itin two different unit sizes i.e. 5 and 25 mg.

    I hope this helps and if I can assist further, please do not hesitate to contact me.

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