Product name(±)-Palmitoylcarnitine chloride, Protein kinase C (PKC) inhibitor
DescriptionProtein kinase C (PKC) inhibitor. Intermediate in lipid metabolism.
Biological descriptionProtein kinase C (PKC) inhibitor. Intermediate in long-chain fatty acid metabolism able to disrupt cell membranes.
Also available in simple stock solutions (ab146730) - add 1 ml of water to get an exact, ready-to-use concentration.
Chemical name3-Carboxy-N,N,N-trimethyl-2-[(1-oxohexadecyl)oxy]-1-propanaminium chloride
Storage instructionsStore at -20°C. Store under desiccating conditions. The product can be stored for up to 12 months.
Solubility overviewSoluble in water to 10 mM
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
Our Abpromise guarantee covers the use of ab141122 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent concentration.|
ab59720 staining ZO1 in Caco-2 cells treated with (±)-palmitoylcarnitine chloride (ab141122), by ICC/IF. Membranar ZO1 expression loss correlates with increased concentration of (±)-palmitoylcarnitine chloride, as described in literature.
The cells were incubated at 37°C for 1 hour in media containing different concentrations of ab141122 ((±)-palmitoylcarnitine chloride) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab59720 (0.5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
This product has been referenced in:
- Nalecz KA et al. Palmitoylcarnitine regulates estrification of lipids and promotes palmitoylation of GAP-43. FEBS Lett 581:3950-4 (2007). Read more (PubMed: 17662726) »
- Requero MA et al. The membrane-perturbing properties of palmitoyl-coenzyme A and palmitoylcarnitine. A comparative study. Biochemistry 34:10400-5 (1995). Read more (PubMed: 7654694) »
- Nakadate T & Blumberg PM Modulation by palmitoylcarnitine of protein kinase C activation. Cancer Res 47:6537-42 (1987). Read more (PubMed: 3479247) »