(±)-Palmitoylcarnitine chloride, Protein kinase C (PKC) inhibitor (ab141122)
Key features and details
- Protein kinase C (PKC) inhibitor. Intermediate in lipid metabolism.
- CAS Number: 6865-14-1
- Purity: > 98%
- Soluble in water to 10 mM
- Form / State: Solid
- Source: Synthetic
Overview
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Product name
(±)-Palmitoylcarnitine chloride, Protein kinase C (PKC) inhibitor -
Description
Protein kinase C (PKC) inhibitor. Intermediate in lipid metabolism. -
Biological description
Protein kinase C (PKC) inhibitor. Intermediate in long-chain fatty acid metabolism able to disrupt cell membranes.
Also available in simple stock solutions (ab146730) - add 1 ml of water to get an exact, ready-to-use concentration. -
Purity
> 98% -
CAS Number
6865-14-1 -
Chemical structure
Properties
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Chemical name
3-Carboxy-N,N,N-trimethyl-2-[(1-oxohexadecyl)oxy]-1-propanaminium chloride -
Molecular weight
436.07 -
Molecular formula
C23H46ClNO4 -
PubChem identifier
363417 -
Storage instructions
Store at -20°C. Store under desiccating conditions. The product can be stored for up to 12 months. -
Solubility overview
Soluble in water to 10 mM -
Handling
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
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SMILES
[Cl-].O=C(CCCCCCCCCCCCCCC)OC(CC(=O)O)C[N+](C)(C)C -
Source
Synthetic
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Research areas
- Epigenetics and Nuclear Signaling
- Nuclear Signaling Pathways
- Nuclear Receptors
- Orphan Nuclear Receptors
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipid metabolism
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab141122 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Functional Studies |
Use at an assay dependent concentration.
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| Notes |
|---|
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Functional Studies
Use at an assay dependent concentration. |
Images
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Chemical Structure - (±)-Palmitoylcarnitine chloride, Protein kinase C (PKC) inhibitor (ab141122)
2D chemical structure of ab141122, (±)-Palmitoylcarnitine chloride, Protein kinase C (PKC) inhibitor
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Functional Studies - (±)-Palmitoylcarnitine chloride, Protein kinase C (PKC) inhibitor (ab141122)ab59720 staining ZO1 in Caco-2 cells treated with (±)-palmitoylcarnitine chloride (ab141122), by ICC/IF. Membranar ZO1 expression loss correlates with increased concentration of (±)-palmitoylcarnitine chloride, as described in literature.
The cells were incubated at 37°C for 1 hour in media containing different concentrations of ab141122 ((±)-palmitoylcarnitine chloride) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab59720 (0.5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
References (0)
ab141122 has not yet been referenced specifically in any publications.