Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (Human embryonic pancreas PCW 10.4)
Permeabilization
Yes - Triton
Specification
Human embryonic pancreas PCW 10.4
Blocking step
(agent) for 1 hour(s) and 0 minute(s) · Concentration: 0.25% · Temperature: 21°C
Fixative
Paraformaldehyde
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 21°C · Diluent: PBS 1x + 0.2% Gelatin +0.25% Triton
Dilution
1/500
Secondary antibody
Name
Abcam antibody:Donkey Anti-Mouse IgG H&L (Alexa Fluor® 555) preadsorbed
Additional data
Additional Notes
Fixation in 4% Paraformaldehyde for one day, cryoprotected in sucrose 10%, Cryostat sections
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Alain Chedotal
Verified customer
Submitted Aug 03 2021