Overview

  • Product name

  • Description

    Rabbit polyclonal to PLXNA3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Rabbit, Chimpanzee, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide within Human PLXNA3 aa 1050-1100. The exact sequence is proprietary.
    Sequence:

    T VQEPRVRAKY RGIETTNTCQ VINDTAMLCK APGIFLGRPQ PRAQGEHPDE


    Database link: P51805

  • Positive control

    • HeLa and 293T whole cell lysates
  • General notes

    Previously labelled as Plexin A3.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituent: 99% Tris citrate/phosphate

    pH: 7 to 8
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab191214 was affinity purified using an epitope specific to PLXNA3 immobilized on solid support.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab191214 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 2-10 µg/mg of lysate.
WB 1/2000 - 1/10000. Predicted molecular weight: 207 kDa.

Target

  • Relevance

    Plexin A3 is a coreceptor for the class 3 secreted semaphorins. This family of semaphorins binds to a complex composed of a neuropilin and a plexin. The plexin type modulates the affinity of the complex for specific semaphorins and its cytoplasmic domain is required for the activation of down-stream signaling events in the cytoplasm. Plexin A3 is a putative receptor involved in the development of neural and epithelial tissues.
  • Cellular localization

    Membrane; single-pass type I membrane protein.
  • Database links

  • Alternative names

    • HSSEXGENE antibody
    • Plexin 4 antibody
    • Plexin A3 antibody
    • PLXN4 antibody
    • PLXNA3 antibody
    • Semaphorin receptor SEX antibody
    • SEX antibody
    • Transmembrane protein sex antibody
    • XAP 6 antibody
    see all

Images

  • All lanes : Anti-PLXNA3 antibody (ab191214) at 0.1 µg/ml

    Lane 1 : HeLa cell lysate in RIPA buffer
    Lane 2 : 293T cell lysate in RIPA buffer
    Lane 3 : Jurkat cell lysate in RIPA buffer

    Lysates/proteins at 50 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 207 kDa


    Exposure time: 3 minutes
  • Immunoprecipitation analysis of HeLa cell lysate (in RIPA buffer, 1.0 mg per IP reaction; 20% of IP loaded) labeling PLXNA3 using ab191214 at 6 μg per reaction.  For blotting immunoprecipitated PLXNA3, ab191214 was used at 1 μg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

References

ab191214 has not yet been referenced specifically in any publications.

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There are currently no Customer reviews or Questions for ab191214.
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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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