Overview

  • Product name
  • Description
    Rabbit polyclonal to PLXNA3
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse
    Predicted to work with: Human
  • Immunogen

    Synthetic peptide corresponding to Mouse PLXNA3 aa 1100-1200.
    (Peptide available as ab41563)

  • Positive control
    • This antibody gave a positive signal in P0 mouse brain and mouse hippocampus tissue lysates
  • General notes

    Previously labelled as Plexin A3.

Properties

Applications

Our Abpromise guarantee covers the use of ab41564 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 207 kDa (predicted molecular weight: 207 kDa).
IHC-P Use at an assay dependent concentration.

Target

  • Relevance
    Plexin A3 is a coreceptor for the class 3 secreted semaphorins. This family of semaphorins binds to a complex composed of a neuropilin and a plexin. The plexin type modulates the affinity of the complex for specific semaphorins and its cytoplasmic domain is required for the activation of down-stream signaling events in the cytoplasm. Plexin A3 is a putative receptor involved in the development of neural and epithelial tissues.
  • Cellular localization
    Membrane; single-pass type I membrane protein.
  • Database links
  • Alternative names
    • HSSEXGENE antibody
    • Plexin 4 antibody
    • Plexin A3 antibody
    • PLXN4 antibody
    • PLXNA3 antibody
    • Semaphorin receptor SEX antibody
    • SEX antibody
    • Transmembrane protein sex antibody
    • XAP 6 antibody
    see all

Images

  • All lanes : Anti-PLXNA3 antibody (ab41564) at 1 µg/ml

    Lane 1 : P0 Mouse Brain Mouse Tissue Lysate
    Lane 2 : Hippocampus (Mouse) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size: 207 kDa
    Observed band size: 207 kDa
    Additional bands at: 32 kDa, 50 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 20 minutes

References

This product has been referenced in:
  • Demyanenko GP  et al. Neural cell adhesion molecule NrCAM regulates Semaphorin 3F-induced dendritic spine remodeling. J Neurosci 34:11274-87 (2014). IHC-FoFr ; Mouse . Read more (PubMed: 25143608) »
See 1 Publication for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Western blot
Sample
Human Cell lysate - whole cell (Cancer cells)
Gel Running Conditions
Reduced Denaturing (10)
Loading amount
22 µg
Specification
Cancer cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Mr. Manoj Kumar Valluru

Verified customer

Submitted Jul 23 2010

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (E 14 Mouse Embryo)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate pH 6
Permeabilization
No
Specification
E 14 Mouse Embryo
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Fixative
Formaldehyde

Mr. Manoj Kumar Valluru

Verified customer

Submitted Nov 03 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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