Overview

  • Product name

    Anti-Plzf antibody [5B3]
    See all Plzf primary antibodies
  • Description

    Mouse monoclonal [5B3] to Plzf
  • Host species

    Mouse
  • Tested applications

    Suitable for: ELISA, WB, ICC/IF, Flow Cytmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Purified recombinant fragment of Human Plzf expressed in E. Coli.

  • Positive control

    • HeLa whole cell lysate (ab150035). HeLa cells
  • General notes

    This product was changed from ascites to supernatant. Lot no’s high than GR161367-22 are from Tissue Culture Supernatant

Properties

Applications

Our Abpromise guarantee covers the use of ab104854 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/10000.
WB 1/500 - 1/2000. Predicted molecular weight: 74 kDa.
ICC/IF Use at an assay dependent concentration.
Flow Cyt 1/50.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Target

  • Function

    Probable transcription factor. May play a role in myeloid maturation and in the development and/or maintenance of other differentiated tissues. Probable substrate-recognition component of an E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins.
  • Tissue specificity

    Within the hematopoietic system, PLZF is expressed in bone marrow, early myeloid cell lines and peripheral blood mononuclear cells. Also expressed in the ovary, and at lower levels, in the kidney and lung.
  • Pathway

    Protein modification; protein ubiquitination.
  • Involvement in disease

    Skeletal defects, genital hypoplasia, and mental retardation
    A chromosomal aberration involving ZBTB16 may be a cause of acute promyelocytic leukemia (APL). Translocation t(11;17)(q32;q21) with RARA.
  • Sequence similarities

    Belongs to the krueppel C2H2-type zinc-finger protein family.
    Contains 1 BTB (POZ) domain.
    Contains 9 C2H2-type zinc fingers.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • AI467657 antibody
    • lu antibody
    • PLZF antibody
    • Promyelocytic leukemia zinc finger protein antibody
    • ZBT16_HUMAN antibody
    • ZBTB16 antibody
    • Zfp145 antibody
    • Zinc finger and BTB domain containing 16 antibody
    • zinc finger and BTB domain containing protein 16 antibody
    • Zinc finger and BTB domain-containing protein 16 antibody
    • Zinc finger protein 145 (Kruppel like expressed in promyelocytic leukemia) antibody
    • Zinc finger protein 145 antibody
    • Zinc finger protein PLZF antibody
    • Znf145 antibody
    see all

Images

  • Anti-Plzf antibody [5B3] (ab104854) at 1/500 dilution + HeLa cell lysate

    Predicted band size: 74 kDa

  • Immunofluorescence analysis of Hela cells using ab104854 (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
  • Overlay histogram showing K562 cells stained with ab104854 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab104854, 1/50 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

References

This product has been referenced in:

  • Yu-Ching Lin Z  et al. Gene expression ontogeny of spermatogenesis in the marmoset uncovers primate characteristics during testicular development. Dev Biol N/A:N/A (2015). IHC-P ; Common marmoset . Read more (PubMed: 25624265) »
  • McConnell MJ  et al. Post transcriptional control of the epigenetic stem cell regulator PLZF by sirtuin and HDAC deacetylases. Epigenetics Chromatin 8:38 (2015). Read more (PubMed: 26405459) »
See all 5 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Immunocytochemistry/ Immunofluorescence
Sample
Monodelphis domestica Cell (testis)
Permeabilization
Yes - 0.5% Triton X-100
Specification
testis
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 23°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Sep 14 2016

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Dako antigen retrieval solution
Sample
Marmoset (common) Tissue sections (Adult testis)
Specification
Adult testis
Permeabilization
No
Fixative
Formaldehyde

Dr. 煜欽 林

Verified customer

Submitted Jun 05 2013

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