• Product name
    Anti-Pma1 antibody [40B7]
  • Description
    Mouse monoclonal [40B7] to Pma1
  • Host species
  • Specificity
    Reacts with Pma1p based on the size of the band detected on western blots and the immunofluorescence localization pattern.
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Saccharomyces cerevisiae
  • Immunogen

    Raised against yeast membrane preps, screened by immunofluorescence and blotting.


  • Form
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.065% Sodium azide
    Constituent: Tissue culture supernatant
  • Purity
    Tissue culture supernatant
  • Purification notes
    Sterile filtered.
  • Clonality
  • Clone number
  • Isotype
  • Light chain type
  • Research areas


Our Abpromise guarantee covers the use of ab4645 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000. Predicted molecular weight: 100 kDa. (cell lysates, ECL detection). For non-ECL western detection methods, 1/1000 - 1/5000. Also detects Pma1p-containing fusion proteins on western blots.
ICC/IF Use at an assay dependent dilution.


  • Relevance
    Pma1p is a plasma membrane ATPase.
  • Database links
    • Alternative names
      • Plasma membrane ATPase 1 antibody
      • Plasma membrane H+ ATPase antibody
      • Pma 1 antibody
      • Pma1p antibody
      • Proton pump 1 antibody
      see all


    • For Pma1 staining cells were grown to a density of 1 A600/ml, rapidly fixed by direct addition of formaldehyde for 30 minutes, and then harvested and fixed overnight with 4.4% formaldehyde in 0.1 m potassium phosphate, pH 6.5. Before staining, fixed cells were permeabilized with 2% SDS. They were then stained with ab4645 followed by incubation with goat anti-mouse IgG conjugated to Alexa Fluor 488. Stained cells were visualized under fluorescein fluorescence optics on a Zeiss Axioplan 2 fluorescence microscope. Nomarski images (left panels) and indirect immunofluorescence (right panels) are shown for each field.


    This product has been referenced in:
    • Geva Y  et al. Two novel effectors of trafficking and maturation of the yeast plasma membrane H+ -ATPase. Traffic 18:672-682 (2017). WB . Read more (PubMed: 28727280) »
    • Simpkins JA  et al. Disruption of a cystine transporter downregulates expression of genes involved in sulfur regulation and cellular respiration. Biol Open 5:689-97 (2016). WB . Read more (PubMed: 27142334) »

    See all 19 Publications for this product

    Customer reviews and Q&As

    Thank you very much for your reply.

    The anti-IgG (H&L) secondary will bind to the conserved light chains of the IgM primary. I suggest using the secondary at a higher concentration than you normally would with an IgG primary, but I'd expe...

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    Thank you very much for contacting us and letting us know about the trouble with this antibody.

    Thank you also for sending so many details about your protocol. I do have a couple of questions, just so I can further understand the situation: <...

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    Thank you for contacting Abcam.

    Under our Abpromise, we guarantee this antibody to work in western on yeast samples. As we had not tested in oh human samples, then we could not guarantee that it would work on this species.

    I would be ...

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    Thank you for your enquiry.

    Below is a selection of antibodies that are suitable for detecting the plasma membrane and nuclear envelope. They all work in western blot and I would review the datasheets to confirm which species they are tested...

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    Merci beaucoup pour votre intérêt pour nos anticorps. Nous avons trois anticorps actuellement qui pourront correspondre a vos besoin. Veuillez vérifier leur fiche technique s'il vous plaît. Je n'ai pas trouvé un anticorps testé contre pichia pa...

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    Abcam guarantees this product to work in the species/application used in this Abreview.
    Western blot
    Saccharomyces cerevisiae Cell lysate - other (Yeast membrane preparation)
    Loading amount
    5 µg
    Yeast membrane preparation
    Gel Running Conditions
    Reduced Denaturing (7.5%)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

    Abcam user community

    Verified customer

    Submitted Aug 07 2009

    Thank you for your enquiry. The isotype of this antibody is IgG3 with kappa light chains. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

    Thank you for your enquiry. All the information we have on species cross reactivity is specified on the datasheet, these are updated as soon as any new information is brought to our attention. As far as we are aware, cross reactivity with Canida a...

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    The Pma1p protein is very antigenic, and antibodies to it turn up frequently if yeast membrane preps are used as the immunogen. However, what the exact antigenic region is, is not currently known.


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