Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-PMCA1 antibody [EPR12029] (ab190355)

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Western blot - Anti-PMCA1 antibody [EPR12029] (ab190355)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PMCA1 antibody [EPR12029] (ab190355)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PMCA1 antibody [EPR12029] (ab190355)
  • Western blot - Anti-PMCA1 antibody [EPR12029] (ab190355)
  • Western blot - Anti-PMCA1 antibody [EPR12029] (ab190355)
  • Anti-PMCA1 antibody [EPR12029] (ab190355)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12029] to PMCA1
  • Suitable for: WB, IHC-P
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-PMCA1 antibody [EPR12029]
    See all PMCA1 primary antibodies

  • Description

    Rabbit monoclonal [EPR12029] to PMCA1

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human PMCA1 aa 1150-1250. The exact sequence is proprietary.
    Database link: P20020

  • Positive control

    • WB: THP-1, HeLa, Neuro-2a, U87-MG and HepG2 cell lysates. IHC-P: Human stomach and Human transitional cell carcinoma of bladder tissues.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab190355 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 130-250 kDa (predicted molecular weight: 139 kDa).
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

  • Function

    This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of calcium out of the cell.

  • Tissue specificity

    Isoform B is ubiquitously expressed. Isoform C is found in brain cortex, skeletal muscle and heart muscle. Isoform D has only been found in fetal skeletal muscle. Isoform K has been found in small intestine and liver.

  • Sequence similarities

    Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIB subfamily.

  • Domain

    The calmodulin-binding subdomain B is different in the different splice variants and shows pH dependent calmodulin binding properties in isoforms A, C, D and E.

  • Cellular localization

    Cell membrane.
  • Information by UniProt

  • Database links

    • Alternative names

      • AT2B1_HUMAN antibody
      • ATP2B1 antibody
      • ATPase Ca++ transporting plasma membrane 1 antibody
      • Plasma membrane calcium ATPase 1 antibody
      • Plasma membrane calcium ATPase isoform 1 antibody
      • Plasma membrane calcium pump isoform 1 antibody
      • Plasma membrane calcium transporting ATPase 1 antibody
      • Plasma membrane calcium-transporting ATPase 1 antibody
      • PMCA 1 antibody
      • PMCA1 antibody
      see all

    Images

    • Western blot - Anti-PMCA1 antibody [EPR12029] (ab190355)
      Western blot - Anti-PMCA1 antibody [EPR12029] (ab190355)
      All lanes : Anti-PMCA1 antibody [EPR12029] (ab190355) at 1/1000 dilution

      Lane 1 : Wild-type HeLa cell lysate
      Lane 2 : ATP2B1 knockout HeLa cell lysate
      Lane 3 : HepG2 cell lysate
      Lane 4 : THP-1 cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

      Predicted band size: 139 kDa
      Observed band size: 180-245 kDa
      why is the actual band size different from the predicted?



      Lanes 1-4: Merged signal (red and green). Green - ab190355 observed at 180-245 kDa. Red - loading control ab8245 observed at 36 kDa. 

       ab190355 Anti-PMCA1 antibody [EPR12029]  was shown to specifically react with PMCA1 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265561 (knockout cell lysate ab257365) was used.  Wild-type and PMCA1 knockout samples were subjected to SDS-PAGE.  ab190355 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated at room temperature for 2. 5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PMCA1 antibody [EPR12029] (ab190355)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PMCA1 antibody [EPR12029] (ab190355)

      Immunohistochemical analysis of paraffin-embedded Human stomach tissue labeling PMCA1 with ab190355 at 1/500 dilution. A prediluted HRP Polymer for Rabbit IgG as secondary antibody. Counterstained with Hematoxylin. Negative control also shown.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PMCA1 antibody [EPR12029] (ab190355)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PMCA1 antibody [EPR12029] (ab190355)

      Immunohistochemical analysis of paraffin-embedded Human transitional cell carcinoma of bladder tissue labeling PMCA1 with ab190355 at 1/500 dilution. A prediluted HRP Polymer for Rabbit IgG as secondary antibody. Counterstained with Hematoxylin. Negative control also shown.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Western blot - Anti-PMCA1 antibody [EPR12029] (ab190355)
      Western blot - Anti-PMCA1 antibody [EPR12029] (ab190355)
      All lanes : Anti-PMCA1 antibody [EPR12029] (ab190355) at 1/2000 dilution

      Lane 1 : U87-MG cell lysate
      Lane 2 : HepG2 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

      Predicted band size: 139 kDa
      Observed band size: 130-250 kDa why is the actual band size different from the predicted?

    • Western blot - Anti-PMCA1 antibody [EPR12029] (ab190355)
      Western blot - Anti-PMCA1 antibody [EPR12029] (ab190355)
      All lanes : Anti-PMCA1 antibody [EPR12029] (ab190355) at 1/2000 dilution

      Lane 1 : HepG2 cell lysate
      Lane 2 : Neuro-2a cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

      Predicted band size: 139 kDa
      Observed band size: 130-250 kDa why is the actual band size different from the predicted?

    • Anti-PMCA1 antibody [EPR12029] (ab190355)
      Anti-PMCA1 antibody [EPR12029] (ab190355)

    References (8)

    Publishing research using ab190355? Please let us know so that we can cite the reference in this datasheet.

    ab190355 has been referenced in 8 publications.

    • Dharmaraj P  et al. Neonatal Hypocalcemic Seizures in Offspring of a Mother With Familial Hypocalciuric Hypercalcemia Type 1 (FHH1). J Clin Endocrinol Metab 105:N/A (2020). PubMed: 32150253
    • Bathala P  et al. Oviductal extracellular vesicles (oviductosomes, OVS) are conserved in humans: murine OVS play a pivotal role in sperm capacitation and fertility. Mol Hum Reprod 24:143-157 (2018). PubMed: 29370405
    • Fereshteh Z  et al. Detection of extracellular vesicles in the mouse vaginal fluid: Their delivery of sperm proteins that stimulate capacitation and modulate fertility. J Cell Physiol N/A:N/A (2018). PubMed: 30536802
    • Gorvin CM  et al. A calcium-sensing receptor mutation causing hypocalcemia disrupts a transmembrane salt bridge to activate ß-arrestin-biased signaling. Sci Signal 11:N/A (2018). PubMed: 29463778
    • Gorvin CM  et al. Calcium-sensing receptor residues with loss- and gain-of-function mutations are located in regions of conformational change and cause signalling bias. Hum Mol Genet 27:3720-3733 (2018). PubMed: 30052933
    • Frandsen SK  et al. Normal and Malignant Cells Exhibit Differential Responses to Calcium Electroporation. Cancer Res 77:4389-4401 (2017). PubMed: 28760856
    • Robertson SYT  et al. Multiple Calcium Export Exchangers and Pumps Are a Prominent Feature of Enamel Organ Cells. Front Physiol 8:336 (2017). WB . PubMed: 28588505
    • Chu W  et al. Lovastatin exerts protective effects on endothelial cells via upregulation of PTK2B. Exp Ther Med 12:1741-1749 (2016). PubMed: 27602089

    Customer reviews and Q&As

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    Western blot abreview for Anti-PMCA1 antibody [EPR12029]

     Inconclusive
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Chicken Tissue lysate - whole (Duodenum)
    Gel Running Conditions
    Reduced Denaturing (4-20%)
    Loading amount
    10 µg
    Specification
    Duodenum
    Blocking step
    Odyssey® Blocking Buffer (PBS) as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Read More

    Mr. Aurelien Brionne

    Verified customer

    Submitted Aug 07 2015

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