Recombinant Anti-PMCA1 antibody [EPR12029] (ab190355)
- Datasheet
- References (7)
- Protocols
Overview
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Product name
Anti-PMCA1 antibody [EPR12029]
See all PMCA1 primary antibodies -
Description
Rabbit monoclonal [EPR12029] to PMCA1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human PMCA1 aa 1150-1250. The exact sequence is proprietary.
Database link: P20020 -
Positive control
- HeLa, Neuro-2a, U87-MG and HepG2 cell lysates; Human stomach and Human transitional cell carcinoma of bladder tissues.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR12029 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab190355 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Detects a band of approximately 130-250 kDa (predicted molecular weight: 139 kDa). | |
| IHC-P | 1/250 - 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Target
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Function
This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of calcium out of the cell. -
Tissue specificity
Isoform B is ubiquitously expressed. Isoform C is found in brain cortex, skeletal muscle and heart muscle. Isoform D has only been found in fetal skeletal muscle. Isoform K has been found in small intestine and liver. -
Sequence similarities
Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIB subfamily. -
Domain
The calmodulin-binding subdomain B is different in the different splice variants and shows pH dependent calmodulin binding properties in isoforms A, C, D and E. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 490 Human
- Entrez Gene: 67972 Mouse
- Entrez Gene: 29598 Rat
- Omim: 108731 Human
- SwissProt: P20020 Human
- SwissProt: P11505 Rat
- Unigene: 506276 Human
- Unigene: 7208 Rat
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Alternative names
- AT2B1_HUMAN antibody
- ATP2B1 antibody
- ATPase Ca++ transporting plasma membrane 1 antibody
see all
Images
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![Western blot - Anti-PMCA1 antibody [EPR12029] (ab190355)](https://www.abcam.com/ps/products/190/ab190355/Images/ab190355-226269-ab190355b.jpg)
Western blot - Anti-PMCA1 antibody [EPR12029] (ab190355)All lanes : Anti-PMCA1 antibody [EPR12029] (ab190355) at 1/2000 dilution
Lane 1 : U87-MG cell lysate
Lane 2 : HepG2 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 139 kDa
Observed band size: 130-250 kDa why is the actual band size different from the predicted? -
![Western blot - Anti-PMCA1 antibody [EPR12029] (ab190355)](https://www.abcam.com/ps/products/190/ab190355/Images/ab190355-226268-ab190355.jpg)
Western blot - Anti-PMCA1 antibody [EPR12029] (ab190355)All lanes : Anti-PMCA1 antibody [EPR12029] (ab190355) at 1/2000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : Neuro-2a cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 139 kDa
Observed band size: 130-250 kDa why is the actual band size different from the predicted? -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PMCA1 antibody [EPR12029] (ab190355)](https://www.abcam.com/ps/products/190/ab190355/Images/ab190355-226271-ab190355d.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PMCA1 antibody [EPR12029] (ab190355)Immunohistochemical analysis of paraffin-embedded Human transitional cell carcinoma of bladder tissue labeling PMCA1 with ab190355 at 1/500 dilution. A prediluted HRP Polymer for Rabbit IgG as secondary antibody. Counterstained with Hematoxylin. Negative control also shown.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PMCA1 antibody [EPR12029] (ab190355)](https://www.abcam.com/ps/products/190/ab190355/Images/ab190355-226270-ab190355c.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PMCA1 antibody [EPR12029] (ab190355)Immunohistochemical analysis of paraffin-embedded Human stomach tissue labeling PMCA1 with ab190355 at 1/500 dilution. A prediluted HRP Polymer for Rabbit IgG as secondary antibody. Counterstained with Hematoxylin. Negative control also shown.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Datasheets and documents
References
This product has been referenced in:
- Fereshteh Z et al. Detection of extracellular vesicles in the mouse vaginal fluid: Their delivery of sperm proteins that stimulate capacitation and modulate fertility. J Cell Physiol N/A:N/A (2018). Read more (PubMed: 30536802) »
- Gorvin CM et al. A calcium-sensing receptor mutation causing hypocalcemia disrupts a transmembrane salt bridge to activate ß-arrestin-biased signaling. Sci Signal 11:N/A (2018). Read more (PubMed: 29463778) »
