Product nameAnti-PME-1 antibody
See all PME-1 primary antibodies
DescriptionRabbit polyclonal to PME-1
Tested applicationsSuitable for: IP, WBmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Cow, Dog, Chimpanzee, Cynomolgus monkey, Rhesus monkey, Gorilla, Orangutan
- HeLa, 293T, Jurkat, mouse TCMK1, and mouse NIH 3T3 whole cell lysates.
Previously labelled as PPME1.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab205956 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at 2-10 µg/mg of lysate.|
|WB||1/2000 - 1/10000. Predicted molecular weight: 18 kDa.|
FunctionDemethylates proteins that have been reversibly carboxymethylated. Demethylates PPP2CB (in vitro).
Sequence similaritiesBelongs to the AB hydrolase superfamily.
- Information by UniProt
- PME 1 antibody
- PME-1 antibody
- PME1 antibody
Detection of PME-1 in Immunoprecipitates of Jurkat whole cell lysates (1 mg for IP, 20% of IP loaded) using ab205956 at 6 µg/mg lysate for IP and 1µg/ml for WB detection. Detection: Chemiluminescence with an exposure time of 30 seconds.
All lanes : Anti-PME-1 antibody (ab205956) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate
Lane 2 : 293T whole cell lysate
Lane 3 : Jurkat whole cell lysate
Lane 4 : TCMK1 whole cell lysate
Lane 5 : NIH 3T3 whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 18 kDa
Exposure time: 3 minutes
ab205956 has not yet been referenced specifically in any publications.