Anti-PME-1 antibody (ab228845)

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Key features and details

  • Rabbit polyclonal to PME-1
  • Suitable for: WB, IHC-P
  • Reacts with: Human
  • Isotype: IgG

Overview

  • Product name

    Anti-PME-1 antibody
    See all PME-1 primary antibodies

  • Description

    Rabbit polyclonal to PME-1

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Chicken, Cow, Rhesus monkey

  • Immunogen

    Recombinant fragment within Human PME-1 (internal sequence). The exact sequence is proprietary.
    Database link: Q9Y570

  • Positive control

    • IHC-P: HeLa xenograft. WB: HEK-293T, A431 and HeLa whole cell lysate.

  • General notes

    Previously labelled as PPME1.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 79.99% PBS, 20% Glycerol (glycerin, glycerine)
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab228845 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000 - 1/20000. Predicted molecular weight: 42 kDa.
IHC-P 1/100 - 1/1000.

Target

Images

  • Western blot - Anti-PME-1 antibody (ab228845)
    Western blot - Anti-PME-1 antibody (ab228845)
    All lanes : Anti-PME-1 antibody (ab228845) at 1/10000 dilution

    Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
    Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell lysate
    Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate

    Developed using the ECL technique.

    Predicted band size: 42 kDa



    10% SDS-PAGE

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PME-1 antibody (ab228845)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PME-1 antibody (ab228845)

    Paraffin-embedded HeLa xenograft stained for PME-1 with ab228845 at a 1/500 dilution in immunohistochemical analysis.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (0)

Publishing research using ab228845? Please let us know so that we can cite the reference in this datasheet.

ab228845 has not yet been referenced specifically in any publications.

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