Anti-PME-1 antibody (ab228845)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-PME-1 antibody
See all PME-1 primary antibodies -
Description
Rabbit polyclonal to PME-1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Chicken, Cow, Rhesus monkey
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Immunogen
Recombinant fragment within Human PME-1 (internal sequence). The exact sequence is proprietary.
Database link: Q9Y570 -
Positive control
- IHC-P: HeLa xenograft. WB: HEK-293T, A431 and HeLa whole cell lysate.
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General notes
Previously labelled as PPME1.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: PBS, 20% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab228845 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/5000 - 1/20000. Predicted molecular weight: 42 kDa. | |
| IHC-P | 1/100 - 1/1000. |
Target
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Function
Demethylates proteins that have been reversibly carboxymethylated. Demethylates PPP2CB (in vitro). -
Sequence similarities
Belongs to the AB hydrolase superfamily. - Information by UniProt
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Database links
- Entrez Gene: 535390 Cow
- Entrez Gene: 51400 Human
- Entrez Gene: 72590 Mouse
- Entrez Gene: 361613 Rat
- Entrez Gene: 718858 Rhesus monkey
- Omim: 611117 Human
- SwissProt: Q58DN4 Cow
- SwissProt: Q9Y570 Human
see all -
Alternative names
- PME 1 antibody
- PME-1 antibody
- PME1 antibody
see all
Images
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Western blot - Anti-PME-1 antibody (ab228845)All lanes : Anti-PME-1 antibody (ab228845) at 1/10000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell lysate
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Developed using the ECL technique.
Predicted band size: 42 kDa10% SDS-PAGE
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PME-1 antibody (ab228845)Paraffin-embedded HeLa xenograft stained for PME-1 with ab228845 at a 1/500 dilution in immunohistochemical analysis.
References
ab228845 has not yet been referenced specifically in any publications.
