Anti-PMF-1 antibody (ab222148)

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Overview

  • Product name

    Anti-PMF-1 antibody
    See all PMF-1 primary antibodies

  • Description

    Rabbit polyclonal to PMF-1

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IFmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment corresponding to Human PMF-1 aa 3-70.
    Sequence:

    EASSANLGSGCEEKRHEGSSSESVPPGTTISRVKLLDTMVDTFLQKLVAA GSYQRFTDCYKCFYQLQP


    Database link: Q6P1K2

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • ICC/IF: HEK-293 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab222148 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml.

Fixation/Permeabilization: PFA/Triton X-100.

Target

  • Function

    Part of the MIS12 complex which is required for normal chromosome alignment and segregation and kinetochore formation during mitosis. May act as a cotranscription partner of NFE2L2 involved in regulation of polyamine-induced transcription of SSAT.

  • Tissue specificity

    Highest levels of expression in heart and skeletal muscle, with significant levels expressed in kidney and liver.

  • Cellular localization

    Nucleus. Chromosome > centromere > kinetochore. Associated with the kinetochore.
  • Information by UniProt

  • Database links

    • Alternative names

      • NNF1R antibody
      • PMF 1 antibody
      • PMF-1 antibody
      • Pmf1 antibody
      • PMF1_HUMAN antibody
      • Polyamine modulated factor 1 antibody
      • Polyamine-modulated factor 1 antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Anti-PMF-1 antibody (ab222148)
      Immunocytochemistry/ Immunofluorescence - Anti-PMF-1 antibody (ab222148)

      PFA-fixed, Triton X-100 permeabilized HEK-293 (human epithelial cell line from embryonic kidney) cells stained for PMF-1 (green) using ab222148 at 4 µg/ml in ICC/IF.

    References

    ab222148 has not yet been referenced specifically in any publications.

    Publishing research using ab222148? Please let us know so that we can cite the reference in this datasheet.

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