Overview

  • Product name

    Anti-PMIS2 antibody
  • Description

    Rabbit polyclonal to PMIS2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Mouse
  • Immunogen

    Synthetic peptide corresponding to Mouse PMIS2 aa 61-80 conjugated to Keyhole Limpet Haemocyanin (KLH).
    Sequence:

    CSNWEDAYRNSSRTMWFNML


    Database link: Q497Q9

  • Positive control

    • Wild-type mouse testis and sperm lysates.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.1% Sodium azide
  • Purity

    Whole antiserum
  • Clonality

    Polyclonal
  • Isotype

    IgG

Applications

Our Abpromise guarantee covers the use of ab194880 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/1000.
WB 1/1000. Predicted molecular weight: 11 kDa.

Target

  • Relevance

    PMIS2 might function both as the ultimate factor regulating sperm transport into the oviducts and in modulating sperm–zona binding.
  • Cellular localization

    integral component of membrane
  • Database links

  • Alternative names

    • 1700071L20Rik antibody
    • 4930479M11Rik antibody
    • Pmis2, sperm specific protein antibody

Images

  • Anti-PMIS2 antibody (ab194880) at 1/1000 dilution + Mouse sperm lysate

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

    Predicted band size: 11 kDa



    SDS-PAGE (10~20% gradient gel).

  • Western blot analysis of PMIS2 protein expression in the lysates of testis and sperm from wild-type (+/+) and PMIS2 knockout (-/-) mice using ab194880 at 1/1000 dilution.

    The difference in predicted molecular mass (11 kDa) and the band position (18 kDa) of PMIS protein might be due to glycosylation.

  • Immunohistochemical analysis of paraffin embedded mouse testis tissue labeling PMIS2 with ab194880 at 1/1000.

    Secondary antibody - Goat anti-rabbit IgG Alexa Fluor 488 conjugated at 1/1000 for 60 minutes.

    DAPI staining for 10 minutes at 1 µg/mL in TBS.

    Blocking with 10% FBS/PBST for 30 mins.

    Washing steps done with PBST (0.25% triton X- 100/PBS) and PBS.

References

ab194880 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab194880.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up