Key features and details
- Rabbit polyclonal to PMIS2
- Suitable for: IHC-P, WB
- Reacts with: Mouse
- Isotype: IgG
Product nameAnti-PMIS2 antibody
DescriptionRabbit polyclonal to PMIS2
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Mouse
- Wild-type mouse testis and sperm lysates.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.1% Sodium azide
Concentration information loading...
Our Abpromise guarantee covers the use of ab194880 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Predicted molecular weight: 11 kDa.|
RelevancePMIS2 might function both as the ultimate factor regulating sperm transport into the oviducts and in modulating sperm–zona binding.
Cellular localizationintegral component of membrane
- 1700071L20Rik antibody
- 4930479M11Rik antibody
- Pmis2, sperm specific protein antibody
Anti-PMIS2 antibody (ab194880) at 1/1000 dilution + Mouse sperm lysate
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Predicted band size: 11 kDa
SDS-PAGE (10~20% gradient gel).
Western blot analysis of PMIS2 protein expression in the lysates of testis and sperm from wild-type (+/+) and PMIS2 knockout (-/-) mice using ab194880 at 1/1000 dilution.
The difference in predicted molecular mass (11 kDa) and the band position (18 kDa) of PMIS protein might be due to glycosylation.
Immunohistochemical analysis of paraffin embedded mouse testis tissue labeling PMIS2 with ab194880 at 1/1000.
Secondary antibody - Goat anti-rabbit IgG Alexa Fluor 488 conjugated at 1/1000 for 60 minutes.
DAPI staining for 10 minutes at 1 µg/mL in TBS.
Blocking with 10% FBS/PBST for 30 mins.
Washing steps done with PBST (0.25% triton X- 100/PBS) and PBS.
ab194880 has not yet been referenced specifically in any publications.