Overview

  • Product name

  • Description

    Rabbit polyclonal to PML Protein
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, IHC-FoFrmore details
  • Species reactivity

    Reacts with: Mouse
  • Immunogen

    Synthetic peptide derived from internal domain (400-500) of mouse PML protein

Properties

Applications

Our Abpromise guarantee covers the use of ab67761 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/5000. Predicted molecular weight: 98 kDa.
IHC-P Use at an assay dependent concentration.
IHC-FoFr Use at an assay dependent concentration. PubMed: 23518714

Target

  • Function

    Key component of PML nuclear bodies that regulate a large number of cellular processes by facilitating post-translational modification of target proteins, promoting protein-protein contacts, or by sequestering proteins. Functions as tumor suppressor. Required for normal, caspase-dependent apoptosis in response to DNA damage, FAS, TNF, or interferons. Plays a role in transcription regulation, DNA damage response, DNA repair and chromatin organization. Plays a role in processes regulated by retinoic acid, regulation of cell division, terminal differentiation of myeloid precursor cells and differentiation of neural progenitor cells. Required for normal immunity to microbial infections. Plays a role in antiviral response. In the cytoplasm, plays a role in TGFB1-dependent processes. Regulates p53/TP53 levels by inhibiting its ubiquitination and proteasomal degradation. Regulates activation of p53/TP53 via phosphorylation at 'Ser-20'. Sequesters MDM2 in the nucleolus after DNA damage, and thereby inhibits ubiquitination and degradation of p53/TP53. Regulates translation of HIF1A by sequestering MTOR, and thereby plays a role in neoangiogenesis and tumor vascularization. Regulates RB1 phosphorylation and activity. Required for normal development of the brain cortex during embryogenesis. Can sequester herpes virus and varicella virus proteins inside PML bodies, and thereby inhibit the formation of infectious viral particles. Regulates phosphorylation of ITPR3 and plays a role in the regulation of calcium homeostasis at the endoplasmic reticulum (By similarity). Regulates transcription activity of ELF4. Inhibits specifically the activity of the tetrameric form of PKM2. Together with SATB1, involved in local chromatin-loop remodeling and gene expression regulation at the MHC-I locus. Regulates PTEN compartmentalization through the inhibition of USP7-mediated deubiquitinylation.
  • Involvement in disease

    Note=A chromosomal aberration involving PML may be a cause of acute promyelocytic leukemia (APL). Translocation t(15;17)(q21;q21) with RARA. The PML breakpoints (type A and type B) lie on either side of an alternatively spliced exon.
  • Sequence similarities

    Contains 2 B box-type zinc fingers.
    Contains 1 RING-type zinc finger.
  • Domain

    Interacts with PKM2 via its coiled-coil domain.
    Binds arsenic via the RING-type zinc finger.
  • Post-translational
    modifications

    Ubiquitinated; mediated by RNF4, SIAH1 or SIAH2 and leading to subsequent proteasomal degradation. 'Lys-6'-, 'Lys-11'-, 'Lys-48'- and 'Lys-63'-linked polyubiquitination by RNF4 is polysumoylation-dependent.
    Undergoes 'Lys-11'-linked sumoylation. Sumoylation on all three sites is required for nuclear body formation. Sumoylation on Lys-160 is a prerequisite for sumoylation on Lys-65. The PML-RARA fusion protein requires the coiled-coil domain for sumoylation. Desumoylated by SENP2 and SENP6. Arsenic induces PML and PML-RARA oncogenic fusion proteins polysumoylation and their subsequent RNF4-dependent ubiquitination and proteasomal degradation, and is used as treatment in acute promyelocytic leukemia (APL).
    Phosphorylated in response to DNA damage, probably by ATR.
    Acetylation may promote sumoylation and enhance induction of apoptosis.
  • Cellular localization

    Nucleus > nucleoplasm. Cytoplasm. Nucleus > PML body. Nucleus > nucleolus. Endoplasmic reticulum membrane. Early endosome membrane. Sumoylated forms localize to the PML nuclear bodies. The B1 box and the RING finger are also required for this nuclear localization. Isoforms lacking a nuclear localization signal are cytoplasmic. Detected in the nucleolus after DNA damage. Sequestered in the cytoplasm by interaction with rabies virus phosphoprotein.
  • Information by UniProt
  • Database links

  • Alternative names

    • Acure promyelocytic leukemia, inducer of antibody
    • MYL antibody
    • Pml antibody
    • PML_HUMAN antibody
    • PP8675 antibody
    • Probable transcription factor PML antibody
    • Promyelocytic leukemia antibody
    • Promyelocytic leukemia inducer of antibody
    • Promyelocytic leukemia protein antibody
    • Protein PML antibody
    • RING finger protein 71 antibody
    • RNF 71 antibody
    • RNF71 antibody
    • TRIM 19 antibody
    • Tripartite motif protein TRIM19 antibody
    • Tripartite motif-containing protein 19 antibody
    see all

Images

  • ab67761 staining PML in PyV-infected 3T3 cells or PML-/- MEFs (MOI of 10–20 pfu/cell), by Immunohistochemistry (PFA perfusion fixed frozen sections).

    At 32 hpi, cells were frozen by high pressure and processed by cryo-substitution for immunoelectron microscopy. Sections were fixed with 0.5% paraformaldehyde for 15 min at 22°C. Sections were blocked in 1% milk/PBST (PBS/0.02% Tween 20) for 30 min at room temperature followed by incubation with primary antibody diluted 1/80 in 1% milk/PBST for 2 hr at 22°C. A gold-conjugated anti-rabbit IgG was used as the secondary antibody (1/20)

References

This product has been referenced in:

  • Jiao X  et al. Dachshund Depletion Disrupts Mammary Gland Development and Diverts the Composition of the Mammary Gland Progenitor Pool. Stem Cell Reports 12:135-151 (2019). Read more (PubMed: 30554919) »
  • Chort A  et al. Interferon beta induces clearance of mutant ataxin 7 and improves locomotion in SCA7 knock-in mice. Brain 136:1732-45 (2013). IHC-Fr, WB, IHC-P ; Mouse, Human . Read more (PubMed: 23518714) »
See all 3 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Answer

J'ai compris que vous aviez rencontré des difficultés avec deux anti-PML et j'aimerais donc vous rappeler que nos produits sont garantis pour fonctionner tels qu'ils sont décrits sur la fiche technique et que nous pouvons donc toujours résoudre un problèmeen vous remboursant, vous faire un avoir, ou vous envoyer un produit de remplacement.

Pourriez-vous, s'il vous plait, me communiquer les informations suivantes :
- la référence de l'anticorps
- le numéro de lot
- le numéro et la date de la commande
- l'application testée
- le type d'échantillon (espèce, lignée cellulaire, tissu)
- quelques détails sur votre protocole (concentration/dilution de l'anticorps, temps d'incubation, agent bloquant, préparation des échantillons, etc.)

Je serai ravi de vous apporter une solution rapide à votre problème.



Autre point, le laboratoire qui produit l'anticorps anti-PML ab67761 m'a informé que malheureusement aucune image Western Blot n'est actuellement disponible. Si vous souhaitezutiliser ab67761 en Western Blot, il me sera possible de vous offrir une offre promotionnelle. Après achat de l'anticorps et l'envoi d’une Abreview avec vos résultats, vous pourrez commander gratuitement n'importe quel anticorps primaire de notre catalogue.
Etapes à suivre :
1. Nous confirmer que vous souhaiteriezutiliser ab67761en Western Blotafin de recevoir le code de remise correspondant. Important, ce code doit être édité avant l’achat de ab67761.
2.Commander ab67761par téléphone, fax ou internet (https://www.abcam.com). Inutile de mentionner le code sur cette commande.
3. Tester l'anticorps
4. Nous faire part de vos résultats grâce à notre système Abreview. Pour plus d’informations https://www.abcam.com/abreviews
5. Après soumission de votre Abreview, appeler notre service clientèle afin de passer votre prochaine commande grâce au code de réduction. Ce code de réduction est utilisable 120 jours après son édition et utilisable lors de l’achat d’un autre anticorps primaire.

N’hésitez pas à nous demander plus d’informations concernant cette offre promotionnelle.

Termes et Conditions : https://www.abcam.com/collaborationdiscount.

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Answer

Merci de nous avoir contactés et désolé pour le délai de ma réponse, j'attendais après le laboratoire les réponses à vos questions.

Concernant l'immunogène : l'information sur la fiche technique est incorrecte. Le peptide utilisé correspond à la régioninterne (entre les acides aminés 400et 500) et non àla région N-terminale. Nous sommes désolés pour cette erreur, la fiche technique va être mise à jour avec la bonneinformation.

J'attends malheureusementtoujours l'image Western Blot pour cet anticorps.

Nous avons2 autresanti-PML garantis pour fonctionner en Western Blot et chez la souris :

ab53773, https://www.abcam.com/ab53773
ab50637, https://www.abcam.com/ab50637



J'espère que ces informations vous seront utiles. N'hésitez pas à nous contacter de nouveau si vous avez d'autres questions.

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