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  1. Link

    pml-protein-antibody-epr16792-ab179466.pdf

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Epigenetics and Nuclear Signaling Transcription Domain Families Zinc Finger
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Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-PML Protein antibody [EPR16792] (ab179466)

  • Datasheet
  • SDS
  • Certificate of Compliance
Reviews (5) Submit a question References (8)

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Immunocytochemistry/ Immunofluorescence - Anti-PML Protein antibody [EPR16792] (ab179466)
  • Western blot - Anti-PML Protein antibody [EPR16792] (ab179466)
  • Western blot - Anti-PML Protein antibody [EPR16792] (ab179466)
  • Western blot - Anti-PML Protein antibody [EPR16792] (ab179466)
  • Immunocytochemistry/ Immunofluorescence - Anti-PML Protein antibody [EPR16792] (ab179466)
  • Western blot - Anti-PML Protein antibody [EPR16792] (ab179466)
  • Western blot - Anti-PML Protein antibody [EPR16792] (ab179466)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PML Protein antibody [EPR16792] (ab179466)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PML Protein antibody [EPR16792] (ab179466)
  • Immunoprecipitation - Anti-PML Protein antibody [EPR16792] (ab179466)
  • Anti-PML Protein antibody [EPR16792] (ab179466)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR16792] to PML Protein
  • Suitable for: IP, ICC/IF, IHC-P, WB
  • Knockout validated
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

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Overview

  • Product name

    Anti-PML Protein antibody [EPR16792]
    See all PML Protein primary antibodies
  • Description

    Rabbit monoclonal [EPR16792] to PML Protein
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, ICC/IF, IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: 293T, K562, HeLa and A549 whole cell lysates; Human fetal brain, fetal heart and fetal kidney lysates; HAP1. IHC-P: Human mammary gland and breast carcinoma tissues. ICC/IF: K562 cells. ICC/IF KO: Hap1 cells (Hap1-PML KO used as a negative cell line). IP: K562 whole cell extract.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR16792
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • Zinc Finger
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Transcription Factors
    • Cancer
    • Cancer Metabolism
    • Response to hypoxia
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Hypoxia

Associated products

  • Alternative Versions

    • Anti-PML Protein antibody [EPR16792] - BSA and Azide free (ab240213)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • KO cell lines

    • Human PML (PML Protein) knockout HeLa cell line (ab261811)
    • Human PML (PML Protein) knockout A549 cell line (ab266980)
  • KO cell lysates

    • Human PML (PML Protein) knockout HeLa cell lysate (ab257081)
    • Human PML (PML Protein) knockout A549 cell lysate (ab257082)
  • KO cell pellets

    • Human PML (PML Protein) knockout HeLa cell pellet (ab278961)
  • Recombinant Protein

    • Recombinant Human PML Protein (ab112341)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab179466 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP
1/70.
ICC/IF (3)
1/500.
IHC-P
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB (2)
1/1000 - 1/2000. Detects a band of approximately 50-110 kDa (predicted molecular weight: 98 kDa).
Notes
IP
1/70.
ICC/IF
1/500.
IHC-P
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB
1/1000 - 1/2000. Detects a band of approximately 50-110 kDa (predicted molecular weight: 98 kDa).

Target

  • Function

    Key component of PML nuclear bodies that regulate a large number of cellular processes by facilitating post-translational modification of target proteins, promoting protein-protein contacts, or by sequestering proteins. Functions as tumor suppressor. Required for normal, caspase-dependent apoptosis in response to DNA damage, FAS, TNF, or interferons. Plays a role in transcription regulation, DNA damage response, DNA repair and chromatin organization. Plays a role in processes regulated by retinoic acid, regulation of cell division, terminal differentiation of myeloid precursor cells and differentiation of neural progenitor cells. Required for normal immunity to microbial infections. Plays a role in antiviral response. In the cytoplasm, plays a role in TGFB1-dependent processes. Regulates p53/TP53 levels by inhibiting its ubiquitination and proteasomal degradation. Regulates activation of p53/TP53 via phosphorylation at 'Ser-20'. Sequesters MDM2 in the nucleolus after DNA damage, and thereby inhibits ubiquitination and degradation of p53/TP53. Regulates translation of HIF1A by sequestering MTOR, and thereby plays a role in neoangiogenesis and tumor vascularization. Regulates RB1 phosphorylation and activity. Required for normal development of the brain cortex during embryogenesis. Can sequester herpes virus and varicella virus proteins inside PML bodies, and thereby inhibit the formation of infectious viral particles. Regulates phosphorylation of ITPR3 and plays a role in the regulation of calcium homeostasis at the endoplasmic reticulum (By similarity). Regulates transcription activity of ELF4. Inhibits specifically the activity of the tetrameric form of PKM2. Together with SATB1, involved in local chromatin-loop remodeling and gene expression regulation at the MHC-I locus. Regulates PTEN compartmentalization through the inhibition of USP7-mediated deubiquitinylation.
  • Involvement in disease

    Note=A chromosomal aberration involving PML may be a cause of acute promyelocytic leukemia (APL). Translocation t(15;17)(q21;q21) with RARA. The PML breakpoints (type A and type B) lie on either side of an alternatively spliced exon.
  • Sequence similarities

    Contains 2 B box-type zinc fingers.
    Contains 1 RING-type zinc finger.
  • Domain

    Interacts with PKM2 via its coiled-coil domain.
    Binds arsenic via the RING-type zinc finger.
  • Post-translational
    modifications

    Ubiquitinated; mediated by RNF4, SIAH1 or SIAH2 and leading to subsequent proteasomal degradation. 'Lys-6'-, 'Lys-11'-, 'Lys-48'- and 'Lys-63'-linked polyubiquitination by RNF4 is polysumoylation-dependent.
    Undergoes 'Lys-11'-linked sumoylation. Sumoylation on all three sites is required for nuclear body formation. Sumoylation on Lys-160 is a prerequisite for sumoylation on Lys-65. The PML-RARA fusion protein requires the coiled-coil domain for sumoylation. Desumoylated by SENP2 and SENP6. Arsenic induces PML and PML-RARA oncogenic fusion proteins polysumoylation and their subsequent RNF4-dependent ubiquitination and proteasomal degradation, and is used as treatment in acute promyelocytic leukemia (APL).
    Phosphorylated in response to DNA damage, probably by ATR.
    Acetylation may promote sumoylation and enhance induction of apoptosis.
  • Cellular localization

    Nucleus > nucleoplasm. Cytoplasm. Nucleus > PML body. Nucleus > nucleolus. Endoplasmic reticulum membrane. Early endosome membrane. Sumoylated forms localize to the PML nuclear bodies. The B1 box and the RING finger are also required for this nuclear localization. Isoforms lacking a nuclear localization signal are cytoplasmic. Detected in the nucleolus after DNA damage. Sequestered in the cytoplasm by interaction with rabies virus phosphoprotein.
  • Target information above from: UniProt accession P29590 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 5371 Human
    • Omim: 102578 Human
    • SwissProt: P29590 Human
    • Unigene: 526464 Human
    • Alternative names

      • Acure promyelocytic leukemia, inducer of antibody
      • MYL antibody
      • Pml antibody
      • PML_HUMAN antibody
      • PP8675 antibody
      • Probable transcription factor PML antibody
      • Promyelocytic leukemia antibody
      • Promyelocytic leukemia inducer of antibody
      • Promyelocytic leukemia protein antibody
      • Protein PML antibody
      • RING finger protein 71 antibody
      • RNF 71 antibody
      • RNF71 antibody
      • TRIM 19 antibody
      • Tripartite motif protein TRIM19 antibody
      • Tripartite motif-containing protein 19 antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Anti-PML Protein antibody [EPR16792] (ab179466)
      Immunocytochemistry/ Immunofluorescence - Anti-PML Protein antibody [EPR16792] (ab179466)

      ab179466 staining PML in wild-type Hap1 cells (top panel) and PML knockout Hap1 cells (bottom panel). The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab179466 at 1/500 dilution and ab7291 (Mouse monoclonal to alpha Tubulin) at 1/1000 dilution overnight at +4°C, followed by a further incubation at room temperature for 1h with a goat secondary antibody to rabbit IgG (Alexa Fluor® 488) (ab150081) at 2 μg/ml (shown in green) and a goat secondary antibody to mouse IgG (Alexa Fluor® 594) (ab150120) at 2 μg/ml (shown in red). Nuclear DNA was labelled in blue with DAPI.

      Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    • Western blot - Anti-PML Protein antibody [EPR16792] (ab179466)
      Western blot - Anti-PML Protein antibody [EPR16792] (ab179466)
      All lanes : Anti-PML Protein antibody [EPR16792] (ab179466) at 1/1000 dilution

      Lane 1 : Wild-type A549 cell lysate
      Lane 2 : PML knockout A549 cell lysate

      Lysates/proteins at 20 µg per lane.

      Performed under reducing conditions.

      Predicted band size: 98 kDa
      Observed band size: 110 kDa why is the actual band size different from the predicted?



      Lanes 1 - 2: Merged signal (red and green). Green - ab179466 observed at 110 kDa. Red - loading control ab7291 (Mouse anti-Alpha Tubulin [DM1A]) observed at 55kDa.

      ab179466 was shown to react with PML in A549 wild-type cells in western blot with loss of signal observed in PML knockout cell line ab266980 (PML knockout cell lysate ab257082). Wild-type and PML knockout A549 cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% Milk before incubation with ab179466 and ab7291 (Mouse anti-Alpha Tubulin [DM1A]) overnight at 4 °C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.

    • Western blot - Anti-PML Protein antibody [EPR16792] (ab179466)
      Western blot - Anti-PML Protein antibody [EPR16792] (ab179466)
      All lanes : Anti-PML Protein antibody [EPR16792] (ab179466) at 1/1000 dilution

      Lane 1 : Wild-type HeLa cell lysate
      Lane 2 : PML knockout HeLa cell lysate
      Lane 3 : 293T cell lysate

      Lysates/proteins at 20 µg per lane.

      Performed under reducing conditions.

      Predicted band size: 98 kDa
      Observed band size: 110 kDa why is the actual band size different from the predicted?



      Lanes 1-3: Merged signal (red and green). Green - ab179466 observed at 50-110 kDa. Red - loading control, ab8245 observed at 37 kDa.

      ab179466 Anti-PML Protein antibody [EPR16792] was shown to specifically react with PML Protein in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab261811 (knockout cell lysate ab257081) was used. Wild-type and PML Protein knockout samples were subjected to SDS-PAGE. ab179466 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.

       

    • Western blot - Anti-PML Protein antibody [EPR16792] (ab179466)
      Western blot - Anti-PML Protein antibody [EPR16792] (ab179466)
      All lanes : Anti-PML Protein antibody [EPR16792] (ab179466) at 1/1000 dilution

      Lane 1 : Wild-type HAP1 whole cell lysate
      Lane 2 : PML knockout HAP1 whole cell lysate
      Lane 3 : HeLa whole cell lysate
      Lane 4 : HEK293 whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Predicted band size: 98 kDa
      Observed band size: 50-110 kDa why is the actual band size different from the predicted?



      Lanes 1 - 4: Merged signal (red and green). Green - ab179466 observed at 50-110 kDa. Red - loading control, ab8245, observed at 37 kDa.

      ab179466 was shown to recognize in wild-type HAP1 cells as signal was lost at the expected MW in PML knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and PML knockout samples were subjected to SDS-PAGE. Ab179466 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

    • Immunocytochemistry/ Immunofluorescence - Anti-PML Protein antibody [EPR16792] (ab179466)
      Immunocytochemistry/ Immunofluorescence - Anti-PML Protein antibody [EPR16792] (ab179466)

      Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized K562 (Human chronic myelogenous leukemia cells from bone marrow) cells labeling PML Protein with ab179466 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/400 dilution (green). Cytoplasm and nuclear staining on K562 cell line is observed. The nuclear counterstain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).

      The negative controls are as follows:-
      -ve control 1 -  ab179466 at 1/500 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
      -ve control 2. - ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/400 dilution.

    • Western blot - Anti-PML Protein antibody [EPR16792] (ab179466)
      Western blot - Anti-PML Protein antibody [EPR16792] (ab179466)
      All lanes : Anti-PML Protein antibody [EPR16792] (ab179466) at 1/20000 dilution

      Lane 1 : 293T (Human epithelial cells from embryonic kidney) whole cell lysate
      Lane 2 : K562 (Human chronic myelogenous leukemia cells from bone marrow) whole cell lysate
      Lane 3 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate
      Lane 4 : A549 (Human lung carcinoma) whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

      Predicted band size: 98 kDa
      Observed band size: 50-110 kDa why is the actual band size different from the predicted?



      Blocking/Dilution buffer: 5% NFDM/TBST.

      ab179466 recognizes 12 isoforms shown as multiple bands ranging from 50 kDa to 110 kDa.

    • Western blot - Anti-PML Protein antibody [EPR16792] (ab179466)
      Western blot - Anti-PML Protein antibody [EPR16792] (ab179466)
      All lanes : Anti-PML Protein antibody [EPR16792] (ab179466) at 1/2000 dilution

      Lane 1 : Human fetal brain lysate
      Lane 2 : Human fetal heart lysate
      Lane 3 : Human fetal kidney lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

      Predicted band size: 98 kDa
      Observed band size: 50-110 kDa why is the actual band size different from the predicted?



      Blocking/Dilution buffer: 5% NFDM/TBST.

      ab179466 recognizes 12 isoforms shown as multiple bands ranging from 50 kDa to 110 kDa.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PML Protein antibody [EPR16792] (ab179466)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PML Protein antibody [EPR16792] (ab179466)

      Immunohistochemical analysis of paraffin-embedded Human mammary gland tissue labeling PML Protein with ab179466 at 1/2000 dilution followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Nucleus staining on epithelial cells of Human mammary gland tissue is observed. Counter stained with Hematoxylin.

      Negative control: Used PBS instead of primary ab, secondary ab is prediluted HRP Polymer for Rabbit/Mouse IgG.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PML Protein antibody [EPR16792] (ab179466)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PML Protein antibody [EPR16792] (ab179466)

      Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling PML Protein with ab179466 at 1/2000 dilution followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin. The breast cancer cells lost expression.

      Reference: Gurrieri C et al. Loss of the Tumor Suppressor PML in Human Cancers of Multiple Histologic Origins. J Natl Cancer Inst 96:269 –279 (2004).

      Negative control: Used PBS instead of primary ab, secondary ab is prediluted HRP Polymer for Rabbit/Mouse IgG.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunoprecipitation - Anti-PML Protein antibody [EPR16792] (ab179466)
      Immunoprecipitation - Anti-PML Protein antibody [EPR16792] (ab179466)

      PML Protein was immunoprecipitated from 1mg of K562 (Human chronic myelogenous leukemia cells from bone marrow) whole cell extract with ab179466 at 1/70 diltuion. Western blot was performed from the immunoprecipitate using ab179466 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution. Lane 1: K562 whole cell extract. Lane 2: PBS instead of K562 whole cell extract.

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

      ab179466 recognizes 12 isoforms shown as multiple bands ranging from 50 kDa to 110 kDa.

    • Anti-PML Protein antibody [EPR16792] (ab179466)
      Anti-PML Protein antibody [EPR16792] (ab179466)

    Protocols

    • Immunoprecipitation protocols
    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

    References (8)

    Publishing research using ab179466? Please let us know so that we can cite the reference in this datasheet.

    ab179466 has been referenced in 8 publications.

    • Rawat P  et al. Stress-induced nuclear condensation of NELF drives transcriptional downregulation. Mol Cell 81:1013-1026.e11 (2021). PubMed: 33548202
    • Zhang L  et al. TiPARP forms nuclear condensates to degrade HIF-1a and suppress tumorigenesis. Proc Natl Acad Sci U S A 117:13447-13456 (2020). PubMed: 32482854
    • Wang Z  et al. Proteomic screening identifies PML/p53 axis as a potential treatment target of facial nerve schwannomas. Am J Transl Res 12:4237-4250 (2020). PubMed: 32913501
    • Cheng J  et al. Interferon Alpha Induces Multiple Cellular Proteins That Coordinately Suppress Hepadnaviral Covalently Closed Circular DNA Transcription. J Virol 94:N/A (2020). PubMed: 32581092
    • Wu W  et al. Downregulation of circ_0132266 in chronic lymphocytic leukemia promoted cell viability through miR-337-3p/PML axis. Aging (Albany NY) 11:3561-3573 (2019). PubMed: 31152142
    • Aichem A  et al. The ubiquitin-like modifier FAT10 interferes with SUMO activation. Nat Commun 10:4452 (2019). PubMed: 31575873
    • Jain A & Vale RD RNA phase transitions in repeat expansion disorders. Nature 546:243-247 (2017). ICC/IF ; Human . PubMed: 28562589
    • Komaravelli N  et al. Respiratory syncytial virus induces NRF2 degradation through a promyelocytic leukemia protein - ring finger protein 4 dependent pathway. Free Radic Biol Med 113:494-504 (2017). PubMed: 29107745

    Customer reviews and Q&As

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    1-5 of 5 Abreviews or Q&A

    Western blot abreview for Anti-PML Protein antibody [EPR16792]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (MC3T3)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    20 µg
    Specification
    MC3T3
    Blocking step
    Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Apr 05 2019

    Western blot abreview for Anti-PML Protein antibody [EPR16792]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (U2OS)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    25 µg
    Specification
    U2OS
    Blocking step
    Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Apr 04 2019

    Immunocytochemistry/ Immunofluorescence abreview for Anti-PML Protein antibody [EPR16792]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (MC3T3)
    Permeabilization
    Yes - NP40
    Specification
    MC3T3
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Apr 04 2019

    Immunocytochemistry/ Immunofluorescence abreview for Anti-PML Protein antibody [EPR16792]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (U2OS)
    Permeabilization
    Yes - NP40
    Specification
    U2OS
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Apr 04 2019

    Immunocytochemistry/ Immunofluorescence abreview for Anti-PML Protein antibody [EPR16792]

    Excellent
    Abreviews
    Abreviews
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (U-2OS)
    Permeabilization
    No
    Specification
    U-2OS
    Blocking step
    BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Fixative
    Methanol
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Jun 14 2017

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