Product nameAnti-PML Protein antibody - N-terminal
See all PML Protein primary antibodies
DescriptionRabbit polyclonal to PML Protein - N-terminal
SpecificityThis antibody detects endogenous levels of total PML protein.
Tested applicationsSuitable for: WB, ELISA, IHC-P, ICC/IF, ChIP, IPmore details
Species reactivityReacts with: Human, African green monkey
- WB: A549 cell extracts. ICC/IF: HeLa cells. ChIP:Chromatin was prepared from modified murine D3 ES cells (an in vitro model of RB1 imprinted expression was generated by integrating human PPP1R26P1 into intron 2 of mouse Rb1 in murine embryonic stem cells (mESCs)). IHC-P: Human normal colon tissue.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS
PBS without Mg2+ and Ca2+
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab53773 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Detects a band of approximately 100 kDa (predicted molecular weight: 69 kDa).|
|IHC-P||Use a concentration of 4 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|ICC/IF||1/100 - 1/250.|
|ChIP||Use at an assay dependent concentration. PubMed: 24019952|
|IP||Use at an assay dependent concentration. PubMed: 23222847|
FunctionKey component of PML nuclear bodies that regulate a large number of cellular processes by facilitating post-translational modification of target proteins, promoting protein-protein contacts, or by sequestering proteins. Functions as tumor suppressor. Required for normal, caspase-dependent apoptosis in response to DNA damage, FAS, TNF, or interferons. Plays a role in transcription regulation, DNA damage response, DNA repair and chromatin organization. Plays a role in processes regulated by retinoic acid, regulation of cell division, terminal differentiation of myeloid precursor cells and differentiation of neural progenitor cells. Required for normal immunity to microbial infections. Plays a role in antiviral response. In the cytoplasm, plays a role in TGFB1-dependent processes. Regulates p53/TP53 levels by inhibiting its ubiquitination and proteasomal degradation. Regulates activation of p53/TP53 via phosphorylation at 'Ser-20'. Sequesters MDM2 in the nucleolus after DNA damage, and thereby inhibits ubiquitination and degradation of p53/TP53. Regulates translation of HIF1A by sequestering MTOR, and thereby plays a role in neoangiogenesis and tumor vascularization. Regulates RB1 phosphorylation and activity. Required for normal development of the brain cortex during embryogenesis. Can sequester herpes virus and varicella virus proteins inside PML bodies, and thereby inhibit the formation of infectious viral particles. Regulates phosphorylation of ITPR3 and plays a role in the regulation of calcium homeostasis at the endoplasmic reticulum (By similarity). Regulates transcription activity of ELF4. Inhibits specifically the activity of the tetrameric form of PKM2. Together with SATB1, involved in local chromatin-loop remodeling and gene expression regulation at the MHC-I locus. Regulates PTEN compartmentalization through the inhibition of USP7-mediated deubiquitinylation.
Involvement in diseaseNote=A chromosomal aberration involving PML may be a cause of acute promyelocytic leukemia (APL). Translocation t(15;17)(q21;q21) with RARA. The PML breakpoints (type A and type B) lie on either side of an alternatively spliced exon.
Sequence similaritiesContains 2 B box-type zinc fingers.
Contains 1 RING-type zinc finger.
DomainInteracts with PKM2 via its coiled-coil domain.
Binds arsenic via the RING-type zinc finger.
modificationsUbiquitinated; mediated by RNF4, SIAH1 or SIAH2 and leading to subsequent proteasomal degradation. 'Lys-6'-, 'Lys-11'-, 'Lys-48'- and 'Lys-63'-linked polyubiquitination by RNF4 is polysumoylation-dependent.
Undergoes 'Lys-11'-linked sumoylation. Sumoylation on all three sites is required for nuclear body formation. Sumoylation on Lys-160 is a prerequisite for sumoylation on Lys-65. The PML-RARA fusion protein requires the coiled-coil domain for sumoylation. Desumoylated by SENP2 and SENP6. Arsenic induces PML and PML-RARA oncogenic fusion proteins polysumoylation and their subsequent RNF4-dependent ubiquitination and proteasomal degradation, and is used as treatment in acute promyelocytic leukemia (APL).
Phosphorylated in response to DNA damage, probably by ATR.
Acetylation may promote sumoylation and enhance induction of apoptosis.
Cellular localizationNucleus > nucleoplasm. Cytoplasm. Nucleus > PML body. Nucleus > nucleolus. Endoplasmic reticulum membrane. Early endosome membrane. Sumoylated forms localize to the PML nuclear bodies. The B1 box and the RING finger are also required for this nuclear localization. Isoforms lacking a nuclear localization signal are cytoplasmic. Detected in the nucleolus after DNA damage. Sequestered in the cytoplasm by interaction with rabies virus phosphoprotein.
- Information by UniProt
- Acure promyelocytic leukemia, inducer of antibody
- MYL antibody
- Pml antibody
All lanes : Anti-PML Protein antibody - N-terminal (ab53773) at 1/500 dilution
Lane 1 : A549 (Human lung carcinoma cell line) cell extracts
Lane 2 : A549 (Human lung carcinoma cell line) cell extracts with immunizing peptide
Predicted band size: 69 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?
ab53773 (1/200) staining PML protein in HeLa (Human epithelial cell line from cervix adenocarcinoma) cells (green). Cells were fixed with methanol, permeabilized with triton X-100/PBS and counterstained with DAPI in order to highlight the nucleus (red). Please refer to abreview for further experimental details.
Chromatin was prepared from modified murine D3 ES cells (an in vitro model of RB1 imprinted expression was generated by integrating human PPP1R26P1 into intron 2 of mouse Rb1 in murine embryonic stem cells (mESCs)). Cells were fixed with formaldehyde for 10 minutes. Chromatin was sheared into fragment sizes ranging from 200 to 600 bp. For immunoprecipitation 50 μl of sheared chromatin was set aside as input and 20-40 μg of sheared chromatin in a volume of 100 μl was used for antibody incubation. 4 µg of ab53773 to PML was used as a non-related control (black bars) and 4 µl of a rabbit polyclonal to RNAPII CTD repeat YSPTSPS (phospho S5) (shaded bars) was added as the test. The immunoprecipitated DNA was quantified at 3 positions on the the retinoblastoma (Rb1) promoter. 1,2 = Rb1_48; 3,4 = Rb1_69 and 5,6 = Rb1_74
ab53773 staining PML in human normal colon tissue. Staining is localized to the nucleus.
Left panel: ab53773 at 4 µg/ml. Right panel: Isotype control.
Sections were stained using an automated system at room temperature. Sections were rehydrated and antigen retrieved with the EDTA pH 9.0 buffer. Slides were blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked for 10 minutes (containing casein 0.25% in PBS), then incubated with primary antibody for 20 minutes, and detected for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with hematoxylin and coverslipped. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
This product has been referenced in:
- Diplas BH et al. The genomic landscape of TERT promoter wildtype-IDH wildtype glioblastoma. Nat Commun 9:2087 (2018). Read more (PubMed: 29802247) »
- Zink LM et al. H3.Y discriminates between HIRA and DAXX chaperone complexes and reveals unexpected insights into human DAXX-H3.3-H4 binding and deposition requirements. Nucleic Acids Res 45:5691-5706 (2017). Read more (PubMed: 28334823) »