Immunocytochemistry/ Immunofluorescence abreview for Anti-PML Protein antibody

Good
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (U2OS human osteosarcoma cell line)
Specification
U2OS human osteosarcoma cell line
Fixative
Formaldehyde
Permeabilization
Yes - triton 0,5% in PBS for 5 min
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C

Other product details

Dilution
1/100
Incubation time
1 hour(s) and 0 minute(s)

Secondary antibody

Name
Non-Abcam antibody was used: alexa fluor anti rabbit 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Dilution
1/500

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Submitted Jan 20 2012

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