Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-PML Protein antibody

Excellent
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Testis)
Specification
Testis
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris-EDTA pH 9.0
Permeabilization
No
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: RT°C

Other product details

Dilution
1/100
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS + 0.3% triton + 0.05% Tween

Secondary antibody

Name
Non-Abcam antibody was used: Jackson Immunoresearch
Host species: Donkey
Clonality: Polyclonal
Conjugation: Biotin
Dilution
1/200

Additional data

Additional Notes
Detection was with avidin-cy2
Left image DAPI/ right image Anti-PML Protein

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Submitted Jul 19 2012

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