Brains were perfused with 4% paraformaldehyde.
Sections cut and stained at 20 microns for immunohistochemistry on slides.
Blocking agent also has 0.5% fish gelatin.
This antibody produced very little background and no bleed-through. It was an excellent stain - very bright and clear, exactly where expected.
Abcam user community
Submitted Jul 22 2013
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