Immunocytochemistry/ Immunofluorescence abreview for Anti-PML Protein antibody

Excellent
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Huh7 liver)
Specification
Huh7 liver
Blocking step
BSA as blocking agent for 20 minute(s) · Concentration: 1% · Temperature: 25°C
Fixative
Paraformaldehyde

Other product details

Incubation time
20 minute(s) · Temperature: 25°C · Diluent: 1%BSA in PBS
Dilution
1/500

Secondary antibody

Name
Non-Abcam antibody was used: Alexaflour 594
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Alexa Fluor® 594
Dilution
1/1000

Additional data

Additional Notes
We used DAPI to counterstain the nucleus. The secondary antibody targeted Rabbit Igg with an Alexafluor 594 conjugation.

Abcam user community

Verified customer

Submitted Nov 17 2014

Sign up