Immunocytochemistry/ Immunofluorescence abreview for Anti-PML Protein antibody

Good
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Specification
HeLa
Fixative
Methanol
Permeabilization
Yes - 0.5% TritonX100 in PBS

Other product details

Dilution
1/200
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 22°C

Secondary antibody

Name
Non-Abcam antibody was used: Goat anti-Rabbit Cy3
Host species: Goat
Clonality: Polyclonal
Conjugation: Cy3 ®
Dilution
1/200

Additional data

Additional Notes
Asynchronous HeLa cells were methanol fixed (-20'C, 10min) and immunofluorescently labeled. Strong nuclear staining patterns were observed (see image).

Dr. Kirk Mcmanus

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Submitted Nov 09 2007

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