Key features and details
- Rabbit polyclonal to PMM1
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-PMM1 antibody
See all PMM1 primary antibodies
DescriptionRabbit polyclonal to PMM1
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Human
Recombinant full length protein corresponding to Human PMM1 aa 2-262.
AVTAQAARRKERVLCLFDVDGTLTPARQKIDPEVAAFLQKLRSRVQIGVV GGSDYCKIAEQLGDGDEVIEKFDYVFAENGTVQYKHGRLLSKQTIQNHLG EELLQDLINFCLSYMALLRLPKKRGTFIEFRNGMLNISPIGRSCTLEERI EFSELDKKEKIREKFVEALKTEFAGKGLRFSRGGMISFDVFPEGWDKRYC LDSLDQDSFDTIHFFGNETSPGGNDFEIFADPRTVGHSVVSPQDTVQRCR EIFFPETAHEA
Database link: Q92871
- WB: HepG2, NIH/3T3 and HEK-293T whole cell lysate; Mouse brain tissue lysate. IHC-P: Human pancreatic tissue.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Purification notesPurity >95%
Our Abpromise guarantee covers the use of ab236109 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/200. Predicted molecular weight: 29 kDa.|
|IHC-P||1/20 - 1/200.|
FunctionInvolved in the synthesis of the GDP-mannose and dolichol-phosphate-mannose required for a number of critical mannosyl transfer reactions. In addition, may be responsible for the degradation of glucose-1,6-bisphosphate in ischemic brain.
Tissue specificityStrong expression in liver, heart, brain, and pancreas; lower expression in skeletal muscle.
PathwayNucleotide-sugar biosynthesis; GDP-alpha-D-mannose biosynthesis; alpha-D-mannose 1-phosphate from D-fructose 6-phosphate: step 2/2.
Sequence similaritiesBelongs to the eukaryotic PMM family.
- Information by UniProt
- Brain glucose-1,6-bisphosphatase antibody
- Phosphomannomutase 1 antibody
- PMM 1 antibody
All lanes : Anti-PMM1 antibody (ab236109) at 1/500 dilution
Lane 1 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 2 : NIH/3T3 (mouse embyro fibroblast cell line) whole cell lysate
Lane 3 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 4 : Mouse brain tissue lysate
All lanes : Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 29 kDa
Paraffin-embedded human pancreatic tissue stained for PMM1 using ab236109 at 1/100 dilution in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab236109 has not yet been referenced specifically in any publications.