Overview

  • Product name

  • Description

    Goat polyclonal to PMP70
  • Host species

    Goat
  • Specificity

    ab129307 is expected to recognize one isoform (NP_002849.1) only.
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Rat, Rabbit, Horse, Hamster, Cow, Dog, Xenopus laevis, Monkey, Gorilla, Opossum, Common marmoset, Orangutan, Bat, Platypus, Elephant
  • Immunogen

    Synthetic peptide:

    C-PDGREDQKRKGISD

    with a Cysteine residue linker, corresponding to internal sequence amino acids 526-539 of Human PMP70 (NP_002849.1).

  • Positive control

    • Human testis tissue; Human Kidney lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab129307 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.2 - 0.6 µg/ml. Predicted molecular weight: 75 kDa.
IHC-P Use a concentration of 2.5 - 3.75 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

Images

  • ab129307 at 3.75 µg/ml, staining PMP70 in Formalin-Fixed, Paraffin-Embedded Human testis tissue by Immunohistochemistry.
  • Anti-PMP70 antibody (ab129307) at 0.2 µg/ml + Human Kidney lysate (in RIPA buffer) at 35 µg

    Developed using the ECL technique.

    Predicted band size: 75 kDa

References

ab129307 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
IHC - Wholemount
Sample
Rat Tissue (Brainstem)
Specification
Brainstem

Abcam user community

Verified customer

Submitted Aug 17 2012

Question
Answer


I am very pleased to hear you would like to accept our offer and test ab129307 in rat. This code will give you: 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for rat and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.
Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.
Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.
The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

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