Anti-PMP70 antibody [CL2524] (ab211533)
Key features and details
- Mouse monoclonal [CL2524] to PMP70
- Suitable for: ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-PMP70 antibody [CL2524]
See all PMP70 primary antibodies -
Description
Mouse monoclonal [CL2524] to PMP70 -
Host species
Mouse -
Tested applications
Suitable for: ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment (proprietary-tag) corresponding to Human PMP70 aa 400-500.
Database link: P28288 -
Epitope
ab211533 binds to an epitope located within the peptide sequence GVQVIPLIPGAGEII (aa 420-434) as determined by overlapping synthetic peptides. -
Positive control
- Human rectum, prostate, liver, kidney, fallopian tube and small intestine tissues; HeLa, A431, MCF7, U2OS and U251 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol, 59% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
CL2524 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab211533 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
Use a concentration of 2 - 10 µg/ml.
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IHC-P |
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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ICC/IF
Use a concentration of 2 - 10 µg/ml. |
IHC-P
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Probable transporter. The nucleotide-binding fold acts as an ATP-binding subunit with ATPase activity. -
Sequence similarities
Belongs to the ABC transporter superfamily. ABCD family. Peroxisomal fatty acyl CoA transporter (TC 3.A.1.203) subfamily.
Contains 1 ABC transmembrane type-1 domain.
Contains 1 ABC transporter domain. -
Cellular localization
Peroxisome membrane. - Information by UniProt
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Database links
- Entrez Gene: 5825 Human
- Omim: 170995 Human
- SwissProt: P28288 Human
- Unigene: 700576 Human
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Alternative names
- 70 kDa peroxisomal membrane protein antibody
- ABC 43 antibody
- ABC D3 antibody
see all
Images
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Immunofluorescent analysis of paraformalin-fixed, Triton X-100 permeabilized U251 cells labeling PMP70 with ab211533 at 4 μg/ml. Microtubule- and nuclear probes are visualized in red and blue respectively.
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Immunofluorescent analysis of paraformalin-fixed, Triton X-100 permeabilized U2OS cells labeling PMP70 with ab211533 at 4 μg/ml dilution. Microtubule- and nuclear probes are visualized in red and blue respectively.
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Immunofluorescent analysis of paraformalin-fixed, Triton X-100 permeabilized MCF7 cells labeling PMP70 with ab211533 at 4 μg/ml dilution. Microtubule- and nuclear probes are visualized in red and blue respectively.
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Immunofluorescent analysis of paraformalin-fixed, Triton X-100 permeabilized A431 cells labeling PMP70 with ab211533 at 4 μg/ml dilution. Microtubule- and nuclear probes are visualized in red and blue respectively.
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Immunofluorescent analysis of paraformalin-fixed, Triton X-100 permeabilized HeLa cells labeling PMP70 with ab211533 at 4 μg/ml dilution. Microtubule- and nuclear probes are visualized in red and blue respectively.
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Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human small intestine tissue labeling PMP70 with ab211533 at 1/200 dilution.
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Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human kidney tissue labeling PMP70 with ab211533 at 1/200 dilution.
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Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human fallopian tube tissue labeling PMP70 with ab211533 at 1/200 dilution.
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Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human liver tissue labeling PMP70 with ab211533 at 1/200 dilution.
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Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human prostate tissue labeling PMP70 with ab211533 at 1/200 dilution.
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Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human rectum tissue labeling PMP70 with ab211533 at 1/200 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (7)
ab211533 has been referenced in 7 publications.
- Dahabieh MS et al. Silencing PEX26 as an unconventional mode to kill drug-resistant cancer cells and forestall drug resistance. Autophagy 18:540-558 (2022). PubMed: 34074205
- Francis V et al. The ARSACS disease protein sacsin controls lysosomal positioning and reformation by regulating microtubule dynamics. J Biol Chem 298:102320 (2022). PubMed: 35933016
- You L et al. Enterovirus 71 induces neural cell apoptosis and autophagy through promoting ACOX1 downregulation and ROS generation. Virulence 11:537-553 (2020). PubMed: 32434419
- Vallese F et al. An expanded palette of improved SPLICS reporters detects multiple organelle contacts in vitro and in vivo. Nat Commun 11:6069 (2020). PubMed: 33247103
- Torres SE et al. Ceapins block the unfolded protein response sensor ATF6a by inducing a neomorphic inter-organelle tether. Elife 8:N/A (2019). PubMed: 31149896
- Cai M et al. Disruption of peroxisome function leads to metabolic stress, mTOR inhibition, and lethality in liver cancer cells. Cancer Lett 421:82-93 (2018). ICC/IF . PubMed: 29458144
- Strauss S et al. Modified aptamers enable quantitative sub-10-nm cellular DNA-PAINT imaging. Nat Methods 15:685-688 (2018). PubMed: 30127504