• Product name
    Anti-PMP70 antibody [EPR5614]
    See all PMP70 primary antibodies
  • Description
    Rabbit monoclonal [EPR5614] to PMP70
  • Host species
  • Tested applications
    Suitable for: WB, ICC, Flow Cytmore details
    Unsuitable for: IHC-P or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human PMP70 aa 600-700 (C terminal). The exact sequence is proprietary.

  • Positive control
    • 293T, SH SY5Y, Caco2, and HepG2 cell lysates; permeabilized HepG2 cells.
  • General notes



    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.



Our Abpromise guarantee covers the use of ab109448 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 70 kDa (predicted molecular weight: 75 kDa).
ICC 1/100 - 1/250.
Flow Cyt 1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

  • Application notes
    Is unsuitable for IHC-P or IP.
  • Target

    • Function
      Probable transporter. The nucleotide-binding fold acts as an ATP-binding subunit with ATPase activity.
    • Sequence similarities
      Belongs to the ABC transporter superfamily. ABCD family. Peroxisomal fatty acyl CoA transporter (TC 3.A.1.203) subfamily.
      Contains 1 ABC transmembrane type-1 domain.
      Contains 1 ABC transporter domain.
    • Cellular localization
      Peroxisome membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • 70 kDa peroxisomal membrane protein antibody
      • ABC 43 antibody
      • ABC D3 antibody
      • ABC43 antibody
      • ABCD 3 antibody
      • Abcd3 antibody
      • ABCD3 protein antibody
      • ABCD3_HUMAN antibody
      • ATP binding cassette sub family D (ALD) member 3 antibody
      • ATP binding cassette sub family D member 3 antibody
      • ATP-binding cassette sub-family D member 3 antibody
      • CBAS5 antibody
      • dJ824O18.1 (ATP-binding cassette, sub family D (ALD), member 3 (PMP70, PXMP1)) antibody
      • Peroxisomal membrane protein 1 (70kD) antibody
      • Peroxisomal membrane protein 1 (70kD, Zellweger syndrome) antibody
      • Peroxisomal membrane protein 1 antibody
      • PMP 70 antibody
      • PMP70 antibody
      • PXMP 1 antibody
      • PXMP1 antibody
      • ZWS2 antibody
      see all


    • All lanes : Anti-PMP70 antibody [EPR5614] (ab109448) at 1/1000 dilution

      Lane 1 : Wild-type HAP1 whole cell lysate
      Lane 2 : ABCD3 (PMP70) knockout HAP1 whole cell lysate
      Lane 3 : A431 whole cell lysate
      Lane 4 : HEPG2 whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Predicted band size: 75 kDa

      Lanes 1 - 4: Merged signal (red and green). Green - ab109448 observed at 75 kDa. Red - loading control, ab9484, observed at 37 kDa.

      ab109448 was shown to specifically react with PMP70 in wild-type HAP1 cells as signal was lost in ABCD3 (PMP70) knockout cells. Wild-type and ABCD3 (PMP70) knockout samples were subjected to SDS-PAGE. Ab109448 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

    • ab109448 at 1/100 dilution staining PMP70 in permeabilized HepG2 cells by Flow cytometry (shown in red). Rabbit IgG negative control (shown in green).
    • All lanes : Anti-PMP70 antibody [EPR5614] (ab109448) at 1/1000 dilution

      Lane 1 : 293T cell lysate
      Lane 2 : SH SY5Y cell lysate
      Lane 3 : Caco2 cell lysate
      Lane 4 : HepG2 cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 75 kDa
      Observed band size: 70 kDa
      why is the actual band size different from the predicted?

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD


    This product has been referenced in:
    • Cai M  et al. Disruption of peroxisome function leads to metabolic stress, mTOR inhibition, and lethality in liver cancer cells. Cancer Lett 421:82-93 (2018). ICC/IF . Read more (PubMed: 29458144) »
    See 1 Publication for this product

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