Anti-PMP70 antibody [EPR5614] (ab109448)
- Datasheet
- References (1)
- Protocols
Overview
-
Product nameAnti-PMP70 antibody [EPR5614]
See all PMP70 primary antibodies -
DescriptionRabbit monoclonal [EPR5614] to PMP70
-
Host speciesRabbit
-
Tested applicationsSuitable for: WB, ICC, Flow Cytmore details
Unsuitable for: IHC-P or IP -
Species reactivityReacts with: Mouse, Rat, Human
-
Immunogen
Synthetic peptide within Human PMP70 aa 600-700 (C terminal). The exact sequence is proprietary.
-
Positive control
- 293T, SH SY5Y, Caco2, and HepG2 cell lysates; permeabilized HepG2 cells.
-
General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
-
FormLiquid
-
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
-
Dissociation constant (KD)KD = 4.10 x 10 -11 M Learn more about KD
-
Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant -
PurityTissue culture supernatant
-
ClonalityMonoclonal
-
Clone numberEPR5614
-
IsotypeIgG
-
Research areas
Associated products
-
Alternative Versions
-
Compatible Secondaries
-
Conjugation kits
-
Isotype control
Applications
Our Abpromise guarantee covers the use of ab109448 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Detects a band of approximately 70 kDa (predicted molecular weight: 75 kDa). | |
| ICC | 1/100 - 1/250. | |
| Flow Cyt | 1/100 - 1/500. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Target
-
FunctionProbable transporter. The nucleotide-binding fold acts as an ATP-binding subunit with ATPase activity.
-
Sequence similaritiesBelongs to the ABC transporter superfamily. ABCD family. Peroxisomal fatty acyl CoA transporter (TC 3.A.1.203) subfamily.
Contains 1 ABC transmembrane type-1 domain.
Contains 1 ABC transporter domain. -
Cellular localizationPeroxisome membrane.
- Information by UniProt
-
Database links
- Entrez Gene: 5825 Human
- Entrez Gene: 19299 Mouse
- Entrez Gene: 25270 Rat
- Omim: 170995 Human
- SwissProt: P28288 Human
- SwissProt: P55096 Mouse
- SwissProt: P16970 Rat
- Unigene: 700576 Human
see all -
Alternative names
- 70 kDa peroxisomal membrane protein antibody
- ABC 43 antibody
- ABC D3 antibody
see all
Images
-
![Western blot - Anti-PMP70 antibody [EPR5614] (ab109448)](https://www.abcam.com/ps/products/109/ab109448/Images/ab109448-331407-ab109448KOWBDWAR.jpg)
Western blot - Anti-PMP70 antibody [EPR5614] (ab109448)All lanes : Anti-PMP70 antibody [EPR5614] (ab109448) at 1/1000 dilution
Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : ABCD3 (PMP70) knockout HAP1 whole cell lysate
Lane 3 : A431 whole cell lysate
Lane 4 : HEPG2 whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 75 kDaLanes 1 - 4: Merged signal (red and green). Green - ab109448 observed at 75 kDa. Red - loading control, ab9484, observed at 37 kDa.
ab109448 was shown to specifically react with PMP70 in wild-type HAP1 cells as signal was lost in ABCD3 (PMP70) knockout cells. Wild-type and ABCD3 (PMP70) knockout samples were subjected to SDS-PAGE. Ab109448 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
-
![Flow Cytometry - Anti-PMP70 antibody [EPR5614] (ab109448)](https://www.abcam.com/ps/products/109/ab109448/Images/PMP70-Primary-antibodies-ab109448-2.jpg)
Flow Cytometry - Anti-PMP70 antibody [EPR5614] (ab109448)ab109448 at 1/100 dilution staining PMP70 in permeabilized HepG2 cells by Flow cytometry (shown in red). Rabbit IgG negative control (shown in green). -
![Western blot - Anti-PMP70 antibody [EPR5614] (ab109448)](https://www.abcam.com/ps/products/109/ab109448/Images/PMP70-Primary-antibodies-ab109448-1.jpg)
Western blot - Anti-PMP70 antibody [EPR5614] (ab109448)All lanes : Anti-PMP70 antibody [EPR5614] (ab109448) at 1/1000 dilution
Lane 1 : 293T cell lysate
Lane 2 : SH SY5Y cell lysate
Lane 3 : Caco2 cell lysate
Lane 4 : HepG2 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 75 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted? -
![Other - Anti-PMP70 antibody [EPR5614] (ab109448)](https://www.abcam.com/ps/products/109/ab109448/Images/PMP70-Primary-antibodies-ab109448-3.jpg)
Other - Anti-PMP70 antibody [EPR5614] (ab109448)
Protocols
Datasheets and documents
References
This product has been referenced in:
- Cai M et al. Disruption of peroxisome function leads to metabolic stress, mTOR inhibition, and lethality in liver cancer cells. Cancer Lett 421:82-93 (2018). ICC/IF . Read more (PubMed: 29458144) »
