Name: Anti-PMP70 antibody [EPR5614]
Brand: RabMAb
SKU: ab109448
Rating: 4 (3 reviews)

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Western blot - Anti-PMP70 antibody [EPR5614] (ab109448)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR5614] to PMP70
Suitable for: WB, Flow Cyt
Knockout validated
Reacts with: Mouse, Human

Product name

Anti-PMP70 antibody [EPR5614]
See all PMP70 primary antibodies
Description

Rabbit monoclonal [EPR5614] to PMP70
Host species

Rabbit
Tested applications

Suitable for: WB, Flow Cytmore details
Unsuitable for: IHC-P or IP
Species reactivity

Reacts with: Mouse, Human
Predicted to work with: Rat
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Hap1, HeLa, 293T, SH SY5Y, Caco2, and HepG2 cell lysates. Flow Cyt: Permeabilized HepG2 cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Dissociation constant (K_D)

K_D = 4.10 x 10 ^-11 M
Learn more about K_D
Storage buffer

pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR5614
Isotype

IgG
Research areas

Alternative Versions
Compatible Secondaries
- Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab109448 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
WB	(3)	1/1000 - 1/10000. Detects a band of approximately 70 kDa (predicted molecular weight: 75 kDa).
Flow Cyt		1/100 - 1/500. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Notes
WB 1/1000 - 1/10000. Detects a band of approximately 70 kDa (predicted molecular weight: 75 kDa).
Flow Cyt 1/100 - 1/500. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Application notes

Is unsuitable for IHC-P or IP.

Function

Probable transporter. The nucleotide-binding fold acts as an ATP-binding subunit with ATPase activity.
Sequence similarities

Belongs to the ABC transporter superfamily. ABCD family. Peroxisomal fatty acyl CoA transporter (TC 3.A.1.203) subfamily.
Contains 1 ABC transmembrane type-1 domain.
Contains 1 ABC transporter domain.
Cellular localization

Peroxisome membrane.
Target information above from: UniProt accession P28288 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 5825 Human
- Entrez Gene: 19299 Mouse
- Entrez Gene: 25270 Rat
- Omim: 170995 Human
- SwissProt: P28288 Human
- SwissProt: P55096 Mouse
- SwissProt: P16970 Rat
- Unigene: 700576 Human
- Unigene: 399042 Mouse
- Unigene: 7024 Rat
see all
Alternative names
- 70 kDa peroxisomal membrane protein antibody
- ABC 43 antibody
- ABC D3 antibody
- ABC43 antibody
- ABCD 3 antibody
- Abcd3 antibody
- ABCD3 protein antibody
- ABCD3_HUMAN antibody
- ATP binding cassette sub family D (ALD) member 3 antibody
- ATP binding cassette sub family D member 3 antibody
- ATP-binding cassette sub-family D member 3 antibody
- CBAS5 antibody
- dJ824O18.1 (ATP-binding cassette, sub family D (ALD), member 3 (PMP70, PXMP1)) antibody
- Peroxisomal membrane protein 1 (70kD) antibody
- Peroxisomal membrane protein 1 (70kD, Zellweger syndrome) antibody
- Peroxisomal membrane protein 1 antibody
- PMP 70 antibody
- PMP70 antibody
- PXMP 1 antibody
- PXMP1 antibody
- ZWS2 antibody
see all

Western blot - Anti-PMP70 antibody [EPR5614] (ab109448)

All lanes : Anti-PMP70 antibody [EPR5614] (ab109448) at 1/1000 dilution

Lane 1 : Wild-type HeLa cell lysate
Lane 2 : ABCD3 knockout HeLa cell lysate

Lysates/proteins at 40 µg per lane.

Performed under reducing conditions.

Predicted band size: 75 kDa
Observed band size: 75 kDa

Lanes 1- 2: Merged signal (red and green). Green - ab109448 observed at 75 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (ab8245) observed at 37 kDa.

ab109448 was shown to react with PMP70 in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line ab265294 (knockout cell lysate ab257134) was used. Wild-type HeLa and ABCD3 knockout HeLa cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab109448 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^®680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
Flow Cytometry - Anti-PMP70 antibody [EPR5614] (ab109448)

ab109448 at 1/100 dilution staining PMP70 in permeabilized HepG2 cells by Flow cytometry (shown in red). Rabbit IgG negative control (shown in green).
Western blot - Anti-PMP70 antibody [EPR5614] (ab109448)

All lanes : Anti-PMP70 antibody [EPR5614] (ab109448) at 1/1000 dilution

Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : ABCD3 (PMP70) knockout HAP1 whole cell lysate
Lane 3 : A431 whole cell lysate
Lane 4 : HEPG2 whole cell lysate

Lysates/proteins at 20 µg per lane.

Predicted band size: 75 kDa

Lanes 1 - 4: Merged signal (red and green). Green - ab109448 observed at 75 kDa. Red - loading control, ab9484, observed at 37 kDa.

ab109448 was shown to specifically react with PMP70 in wild-type HAP1 cells as signal was lost in ABCD3 (PMP70) knockout cells. Wild-type and ABCD3 (PMP70) knockout samples were subjected to SDS-PAGE. Ab109448 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
Western blot - Anti-PMP70 antibody [EPR5614] (ab109448)

All lanes : Anti-PMP70 antibody [EPR5614] (ab109448) at 1/1000 dilution

Lane 1 : 293T cell lysate
Lane 2 : SH SY5Y cell lysate
Lane 3 : Caco2 cell lysate
Lane 4 : HepG2 cell lysate

Lysates/proteins at 10 µg per lane.

Predicted band size: 75 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted?
OI-RD Scanning - Anti-PMP70 antibody [EPR5614] (ab109448)

Equilibrium disassociation constant (K_D)
Learn more about K_D

Click here to learn more about K_D
Anti-PMP70 antibody [EPR5614] (ab109448)

Western blot protocols
Flow cytometry protocols
Immunocytochemistry & immunofluorescence protocols

Click here to view the general protocols

SDS download

Country/Region

Language
Datasheet download

Download

Publishing research using ab109448? Please let us know so that we can cite the reference in this datasheet.

ab109448 has been referenced in 4 publications.

Covill-Cooke C et al. Peroxisomal fission is modulated by the mitochondrial Rho-GTPases, Miro1 and Miro2. EMBO Rep 21:e49865 (2020). PubMed: 31894645
Torres SE et al. Ceapins block the unfolded protein response sensor ATF6a by inducing a neomorphic inter-organelle tether. Elife 8:N/A (2019). PubMed: 31149896
Chang CL et al. Spastin tethers lipid droplets to peroxisomes and directs fatty acid trafficking through ESCRT-III. J Cell Biol 218:2583-2599 (2019). PubMed: 31227594
Cai M et al. Disruption of peroxisome function leads to metabolic stress, mTOR inhibition, and lethality in liver cancer cells. Cancer Lett 421:82-93 (2018). ICC/IF . PubMed: 29458144

Customer reviews and Q&As

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1-3 of 3 Abreviews or Q&A

Application

Western blot

Sample

Human Cell lysate - whole cell (kidney)

Gel Running Conditions

Reduced Denaturing (4-12% bis-tris gel, MES buffer)

Loading amount

30 µg

Specification

kidney

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Abcam user community

Verified customer

Submitted Nov 03 2020

Application

Western blot

Sample

Human Cell lysate - whole cell (Dopaminergic Neurons)

Gel Running Conditions

Reduced Denaturing

Loading amount

30 µg

Specification

Dopaminergic Neurons

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Abcam user community

Verified customer

Submitted Oct 19 2020

Application

Western blot

Sample

Human Cell lysate - whole cell (HEK293T)

Gel Running Conditions

Reduced Denaturing (10% gel)

Loading amount

20 µg

Specification

HEK293T

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Abcam user community

Verified customer

Submitted Apr 29 2019

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Key features and details

Overview

Product name

Description

Host species

Tested applications

Species reactivity

Immunogen

Positive control

General notes

Properties

Form

Storage instructions

Dissociation constant (KD)

Storage buffer

Purity

Clonality

Clone number

Isotype

Research areas

Associated products

Alternative Versions

Compatible Secondaries

Isotype control

Applications

The Abpromise guarantee

Target

Function

Sequence similarities

Cellular localization

Database links

Alternative names

Images

Protocols

Datasheets and documents

SDS download

Datasheet download

References (4)

Customer reviews and Q&As

Western blot abreview for Anti-PMP70 antibody [EPR5614]

Western blot abreview for Anti-PMP70 antibody [EPR5614]

Western blot abreview for Anti-PMP70 antibody [EPR5614]

Dissociation constant (K_D)