Product nameAnti-PMPCA/INPP5E antibody
See all PMPCA/INPP5E primary antibodies
DescriptionRabbit polyclonal to PMPCA/INPP5E
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Cow, Orangutan
Synthetic peptide within Human PMPCA/INPP5E aa 25-75. The exact sequence is proprietary.
Database link: Q10713
- WB: HeLa, HEK-293T and Jurkat whole cell lysate. IP: HEK-293T whole cell lysate.
This product was previously labelled as PMPCA
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
Concentration information loading...
PurityImmunogen affinity purified
Purification notesAntibody was affinity purified using an epitope specific to PMPCA/INPP5E immobilized on solid support
Our Abpromise guarantee covers the use of ab241368 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 58 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
FunctionCleaves presequences (transit peptides) from mitochondrial protein precursors.
Sequence similaritiesBelongs to the peptidase M16 family.
Cellular localizationMitochondrion matrix.
- Information by UniProt
- 1200002L24Rik antibody
- 4933435E07Rik antibody
- Alpha MPP antibody
All lanes : Anti-PMPCA/INPP5E antibody (ab241368) at 0.1 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 58 kDa
Exposure time: 30 seconds
Lysates prepared in NETN buffer.
PMPCA/INPP5E was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded, prepared using NETN lysis buffer) using ab241368 at 6 µg per reaction. Western blot was performed on the immunoprecipitate using ab241368 at 1 µg/ml.
Lane 1: ab241368 IP in HEK-293T whole cell lysate.
lane 2: Control IgG IP in HEK-293T whole cell lysate.
Detection: Chemiluminescence with an exposure time of 3 minutes.
ab241368 has not yet been referenced specifically in any publications.