Overview

  • Product name

    Anti-PMPCA/INPP5E antibody
    See all PMPCA/INPP5E primary antibodies
  • Description

    Rabbit polyclonal to PMPCA/INPP5E
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Cow, Orangutan
  • Immunogen

    Synthetic peptide within Human PMPCA/INPP5E aa 25-75. The exact sequence is proprietary.
    Database link: Q10713

  • Positive control

    • WB: HeLa, HEK-293T and Jurkat whole cell lysate. IP: HEK-293T whole cell lysate.
  • General notes

     This product was previously labelled as PMPCA

     

Properties

Applications

Our Abpromise guarantee covers the use of ab241368 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 58 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

  • Function

    Cleaves presequences (transit peptides) from mitochondrial protein precursors.
  • Sequence similarities

    Belongs to the peptidase M16 family.
  • Cellular localization

    Mitochondrion matrix.
  • Information by UniProt
  • Database links

  • Alternative names

    • 1200002L24Rik antibody
    • 4933435E07Rik antibody
    • Alpha MPP antibody
    • Alpha-MPP antibody
    • FLJ26258 antibody
    • Inositol polyphosphate 5 phosphatase, 72 kD antibody
    • INPP5E antibody
    • KIAA0123 antibody
    • MGC104197 antibody
    • MGC93916 antibody
    • Mitochondrial matrix processing protease, alpha subunit antibody
    • mitochondrial processing peptidase subunit alpha antibody
    • Mitochondrial-processing peptidase subunit alpha antibody
    • MPPA_HUMAN antibody
    • P 55 antibody
    • P-55 antibody
    • Peptidase (mitochondrial processing) alpha antibody
    • pmpca antibody
    • RP11-413M3.1 antibody
    • RP23-306D20.8 antibody
    • SCAR2 antibody
    see all

Images

  • All lanes : Anti-PMPCA/INPP5E antibody (ab241368) at 0.1 µg/ml

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
    Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate

    Lysates/proteins at 50 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 58 kDa


    Exposure time: 30 seconds


    Lysates prepared in NETN buffer.

  • PMPCA/INPP5E was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded, prepared using NETN lysis buffer) using ab241368 at 6 µg per reaction. Western blot was performed on the immunoprecipitate using ab241368 at 1 µg/ml.

    Lane 1: ab241368 IP in HEK-293T whole cell lysate.
    lane 2: Control IgG IP in HEK-293T whole cell lysate.

    Detection: Chemiluminescence with an exposure time of 3 minutes.

     

     

References

ab241368 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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