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  1. Link

    pms1-antibody-epr8058-ab129020.pdf

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Epigenetics and Nuclear Signaling DNA / RNA DNA Damage & Repair Mismatch Repair
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RecombinantRabMAb

Recombinant Anti-PMS1 antibody [EPR8058] (ab129020)

  • Datasheet
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Western blot - Anti-PMS1 antibody [EPR8058] (ab129020)
  • Flow Cytometry - Anti-PMS1 antibody [EPR8058] (ab129020)
  • OI-RD Scanning - Anti-PMS1 antibody [EPR8058] (ab129020)
  • Anti-PMS1 antibody [EPR8058] (ab129020)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR8058] to PMS1
  • Suitable for: WB, Flow Cyt
  • Reacts with: Human

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Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

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Overview

  • Product name

    Anti-PMS1 antibody [EPR8058]
    See all PMS1 primary antibodies
  • Description

    Rabbit monoclonal [EPR8058] to PMS1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, Flow Cytmore details
    Unsuitable for: ICC,IHC-P or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human PMS1 aa 50-150. The exact sequence is proprietary.

  • Positive control

    • HeLa, Jurkat, Molt-4, HepG2 whole cell lysate (ab7900).
  • General notes

    To see more of the key markers and tools you need to study the hallmarks of cancer, including genome instability and mutation, please visit the following page.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Dissociation constant (KD)

    KD = 7.10 x 10 -11 M
    Learn more about KD
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR8058
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • DNA Damage & Repair
    • Mismatch Repair

Associated products

  • Alternative Versions

    • Anti-PMS1 antibody [EPR8058] - BSA and Azide free (ab248265)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • HeLa nuclear extract lysate (ab14655)
    • HepG2 nuclear extract lysate (ab14660)
    • MOLT4 whole cell lysate (ab7912)

Applications

Our Abpromise guarantee covers the use of ab129020 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Predicted molecular weight: 106 kDa.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for ICC,IHC-P or IP.
  • Target

    • Function

      Probably involved in the repair of mismatches in DNA.
    • Involvement in disease

      Defects in PMS1 are the cause of hereditary non-polyposis colorectal cancer type 3 (HNPCC3) [MIM:600258]. Mutations in more than one gene locus can be involved alone or in combination in the production of the HNPCC phenotype (also called Lynch syndrome). Most families with clinically recognized HNPCC have mutations in either MLH1 or MSH2 genes. HNPCC is an autosomal, dominantly inherited disease associated with marked increase in cancer susceptibility. It is characterized by a familial predisposition to early onset colorectal carcinoma (CRC) and extra-colonic cancers of the gastrointestinal, urological and female reproductive tracts. HNPCC is reported to be the most common form of inherited colorectal cancer in the Western world, and accounts for 15% of all colon cancers. Cancers in HNPCC originate within benign neoplastic polyps termed adenomas. Clinically, HNPCC is often divided into two subgroups. Type I: hereditary predisposition to colorectal cancer, a young age of onset, and carcinoma observed in the proximal colon. Type II: patients have an increased risk for cancers in certain tissues such as the uterus, ovary, breast, stomach, small intestine, skin, and larynx in addition to the colon. Diagnosis of classical HNPCC is based on the Amsterdam criteria: 3 or more relatives affected by colorectal cancer, one a first degree relative of the other two; 2 or more generation affected; 1 or more colorectal cancers presenting before 50 years of age; exclusion of hereditary polyposis syndromes. The term 'suspected HNPCC' or 'incomplete HNPCC' can be used to describe families who do not or only partially fulfill the Amsterdam criteria, but in whom a genetic basis for colon cancer is strongly suspected.
    • Sequence similarities

      Belongs to the DNA mismatch repair mutL/hexB family.
      Contains 1 HMG box DNA-binding domain.
    • Cellular localization

      Nucleus.
    • Target information above from: UniProt accession P54277 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 5378 Human
      • NCBI: 4505911 Human
      • Omim: 600258 Human
      • SwissProt: P54277 Human
      • Unigene: 111749 Human
      • Alternative names

        • DNA mismatch repair protein PMS1 antibody
        • FLJ98259 antibody
        • HNPCC3 antibody
        • hPMS1 antibody
        • Human homolog of yeast mutL antibody
        • Mismatch repair gene PMSL1 antibody
        • pms1 antibody
        • PMS1 postmeiotic segregation increased 1 (S. cerevisiae) antibody
        • PMS1 postmeiotic segregation increased 1 antibody
        • PMS1 protein homolog 1 antibody
        • PMS1_HUMAN antibody
        • PMSL1 antibody
        • Rhabdomyosarcoma antigen MU RMS 40.10B antibody
        • Rhabdomyosarcoma antigen MU RMS 40.10E antibody
        see all

      Images

      • Western blot - Anti-PMS1 antibody [EPR8058] (ab129020)
        Western blot - Anti-PMS1 antibody [EPR8058] (ab129020)
        All lanes : Anti-PMS1 antibody [EPR8058] (ab129020) at 1/10000 dilution

        Lane 1 : HeLa cell lysates
        Lane 2 : Jurkat cell lysates
        Lane 3 : Molt-4 cell lysates
        Lane 4 : HepG2 cell lysates

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : HRP labelled goat anti-rabbit

        Predicted band size: 106 kDa
        Observed band size: 105 kDa
        why is the actual band size different from the predicted?

      • Flow Cytometry - Anti-PMS1 antibody [EPR8058] (ab129020)
        Flow Cytometry - Anti-PMS1 antibody [EPR8058] (ab129020)
        Flow cytometric analysis of permeabilized Jurkat cells using ab129020 at 1/10 (red) or a rabbit IgG negative (green).
      • OI-RD Scanning - Anti-PMS1 antibody [EPR8058] (ab129020)
        OI-RD Scanning - Anti-PMS1 antibody [EPR8058] (ab129020)
        Equilibrium disassociation constant (KD)
        Learn more about KD

        Click here to learn more about KD
      • Anti-PMS1 antibody [EPR8058] (ab129020)
        Anti-PMS1 antibody [EPR8058] (ab129020)

      Protocols

      • Western blot protocols
      • Flow cytometry protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
    • References (0)

      Publishing research using ab129020? Please let us know so that we can cite the reference in this datasheet.

      ab129020 has not yet been referenced specifically in any publications.

      Customer reviews and Q&As

      Show All Reviews Q&A
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      There are currently no Customer reviews or Questions for ab129020.
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