Recombinant Anti-PMS1 antibody [EPR8058] (ab129020)
![Western blot - Anti-PMS1 antibody [EPR8058] (ab129020)](https://www.abcam.com/ps/products/129/ab129020/Images/ab129020-142944-anti-pms1-antibody-epr8058-western-blot.jpg "Western blot - Anti-PMS1 antibody [EPR8058] (ab129020)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8058] to PMS1
- Suitable for: WB, Flow Cyt
- Reacts with: Human
Overview
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Product name
Anti-PMS1 antibody [EPR8058]
See all PMS1 primary antibodies -
Description
Rabbit monoclonal [EPR8058] to PMS1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, Flow Cytmore details
Unsuitable for: ICC,IHC-P or IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human PMS1 aa 50-150. The exact sequence is proprietary.
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Positive control
- HeLa, Jurkat, Molt-4, HepG2 whole cell lysate (ab7900).
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General notes
To see more of the key markers and tools you need to study the hallmarks of cancer, including genome instability and mutation, please visit the following page.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 7.10 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR8058 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab129020 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/10000 - 1/50000. Predicted molecular weight: 106 kDa. | |
| Flow Cyt | 1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|
Target
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Function
Probably involved in the repair of mismatches in DNA. -
Involvement in disease
Defects in PMS1 are the cause of hereditary non-polyposis colorectal cancer type 3 (HNPCC3) [MIM:600258]. Mutations in more than one gene locus can be involved alone or in combination in the production of the HNPCC phenotype (also called Lynch syndrome). Most families with clinically recognized HNPCC have mutations in either MLH1 or MSH2 genes. HNPCC is an autosomal, dominantly inherited disease associated with marked increase in cancer susceptibility. It is characterized by a familial predisposition to early onset colorectal carcinoma (CRC) and extra-colonic cancers of the gastrointestinal, urological and female reproductive tracts. HNPCC is reported to be the most common form of inherited colorectal cancer in the Western world, and accounts for 15% of all colon cancers. Cancers in HNPCC originate within benign neoplastic polyps termed adenomas. Clinically, HNPCC is often divided into two subgroups. Type I: hereditary predisposition to colorectal cancer, a young age of onset, and carcinoma observed in the proximal colon. Type II: patients have an increased risk for cancers in certain tissues such as the uterus, ovary, breast, stomach, small intestine, skin, and larynx in addition to the colon. Diagnosis of classical HNPCC is based on the Amsterdam criteria: 3 or more relatives affected by colorectal cancer, one a first degree relative of the other two; 2 or more generation affected; 1 or more colorectal cancers presenting before 50 years of age; exclusion of hereditary polyposis syndromes. The term 'suspected HNPCC' or 'incomplete HNPCC' can be used to describe families who do not or only partially fulfill the Amsterdam criteria, but in whom a genetic basis for colon cancer is strongly suspected. -
Sequence similarities
Belongs to the DNA mismatch repair mutL/hexB family.
Contains 1 HMG box DNA-binding domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 5378 Human
- NCBI: 4505911 Human
- Omim: 600258 Human
- SwissProt: P54277 Human
- Unigene: 111749 Human
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Alternative names
- DNA mismatch repair protein PMS1 antibody
- FLJ98259 antibody
- HNPCC3 antibody
see all
Images
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Western blot - Anti-PMS1 antibody [EPR8058] (ab129020)All lanes : Anti-PMS1 antibody [EPR8058] (ab129020) at 1/10000 dilution
Lane 1 : HeLa cell lysates
Lane 2 : Jurkat cell lysates
Lane 3 : Molt-4 cell lysates
Lane 4 : HepG2 cell lysates
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit
Predicted band size: 106 kDa
Observed band size: 105 kDa why is the actual band size different from the predicted? -
![Flow Cytometry - Anti-PMS1 antibody [EPR8058] (ab129020)](https://www.abcam.com/ps/products/129/ab129020/Images/ab129020-142943-anti-pms1-antibody-epr8058-flow-cytometry.jpg)
Flow Cytometry - Anti-PMS1 antibody [EPR8058] (ab129020)Flow cytometric analysis of permeabilized Jurkat cells using ab129020 at 1/10 (red) or a rabbit IgG negative (green). -
![OI-RD Scanning - Anti-PMS1 antibody [EPR8058] (ab129020)](https://www.abcam.com/ps/products/129/ab129020/Images/ab129020-142942-anti-pms1-antibody-epr8058.jpg)
OI-RD Scanning - Anti-PMS1 antibody [EPR8058] (ab129020) -
![Anti-PMS1 antibody [EPR8058] (ab129020)](https://www.abcam.com/ps/products/129/ab129020/Images/ab129020-4-benefits-of-recombinant-antibodies.png)
Anti-PMS1 antibody [EPR8058] (ab129020)
Datasheets and documents
References (0)
ab129020 has not yet been referenced specifically in any publications.
