Product nameAnti-PMS1 antibody [EPR8058]
See all PMS1 primary antibodies
DescriptionRabbit monoclonal [EPR8058] to PMS1
Tested applicationsSuitable for: WB, Flow Cytmore details
Unsuitable for: ICC,IHC-P or IP
Species reactivityReacts with: Human
Synthetic peptide within Human PMS1 aa 50-150. The exact sequence is proprietary.
- HeLa, Jurkat, Molt-4, HepG2 whole cell lysate (ab7900).
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Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Dissociation constant (KD)KD = 7.10 x 10 -11 M Learn more about KD
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab129020 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/10000 - 1/50000. Predicted molecular weight: 106 kDa.|
|Flow Cyt||1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionProbably involved in the repair of mismatches in DNA.
Involvement in diseaseDefects in PMS1 are the cause of hereditary non-polyposis colorectal cancer type 3 (HNPCC3) [MIM:600258]. Mutations in more than one gene locus can be involved alone or in combination in the production of the HNPCC phenotype (also called Lynch syndrome). Most families with clinically recognized HNPCC have mutations in either MLH1 or MSH2 genes. HNPCC is an autosomal, dominantly inherited disease associated with marked increase in cancer susceptibility. It is characterized by a familial predisposition to early onset colorectal carcinoma (CRC) and extra-colonic cancers of the gastrointestinal, urological and female reproductive tracts. HNPCC is reported to be the most common form of inherited colorectal cancer in the Western world, and accounts for 15% of all colon cancers. Cancers in HNPCC originate within benign neoplastic polyps termed adenomas. Clinically, HNPCC is often divided into two subgroups. Type I: hereditary predisposition to colorectal cancer, a young age of onset, and carcinoma observed in the proximal colon. Type II: patients have an increased risk for cancers in certain tissues such as the uterus, ovary, breast, stomach, small intestine, skin, and larynx in addition to the colon. Diagnosis of classical HNPCC is based on the Amsterdam criteria: 3 or more relatives affected by colorectal cancer, one a first degree relative of the other two; 2 or more generation affected; 1 or more colorectal cancers presenting before 50 years of age; exclusion of hereditary polyposis syndromes. The term 'suspected HNPCC' or 'incomplete HNPCC' can be used to describe families who do not or only partially fulfill the Amsterdam criteria, but in whom a genetic basis for colon cancer is strongly suspected.
Sequence similaritiesBelongs to the DNA mismatch repair mutL/hexB family.
Contains 1 HMG box DNA-binding domain.
- Information by UniProt
- DNA mismatch repair protein PMS1 antibody
- FLJ98259 antibody
- HNPCC3 antibody
All lanes : Anti-PMS1 antibody [EPR8058] (ab129020) at 1/10000 dilution
Lane 1 : HeLa cell lysates
Lane 2 : Jurkat cell lysates
Lane 3 : Molt-4 cell lysates
Lane 4 : HepG2 cell lysates
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled goat anti-rabbit
Predicted band size: 106 kDa
Observed band size: 105 kDa why is the actual band size different from the predicted?
Flow cytometric analysis of permeabilized Jurkat cells using ab129020 at 1/10 (red) or a rabbit IgG negative (green).
Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD
ab129020 has not yet been referenced specifically in any publications.