Overview

  • Product name

    PMSF, protease inhibitor
  • Description

    Non-specific protease inhibitor
  • Alternative names

    • Phenylmethylsulfonyl fluoride
  • Biological description

    Non-specific protease inhibitor; binds key active site serine residue in serine proteases, acetylcholinesterases, and thiol proteases. Suitable for use in vitro; in the preparation of cell lysates, and in vivo where it has been shown to block anandamide (ab120429) metabolism. Able to rapidly cross the BBB.
  • Purity

    > 99%
  • General notes

    Unstable in water

  • CAS Number

    329-98-6
  • Chemical structure

    Chemical Structure

Properties

  • Chemical name

    Phenylmethylsulfonyl fluoride
  • Molecular weight

    174.19
  • Molecular formula

    C7H7FO2S
  • PubChem identifier

    4784
  • Storage instructions

    Store at Room Temperature. Store under desiccating conditions. The product can be stored for up to 12 months.
  • Solubility overview

    Soluble in ethanol to 100 mM and in DMSO to 100 mM
  • Handling

    Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.

    Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.

  • SMILES

    O=S(F)(=O)Cc1ccccc1
  • Source

    Synthetic

  • Research areas

Images

  • Analysis of protein expression level in the sclera. The scleral tissue was homogenized and solubilized in ice-cold PBS containing PMSF protease inhibitor. Lumican and collagen type I were both overexpressed at the protein level in the transgenic sclera. GAPDH was used as a control. Lane 1 to lane 5, transgenic mice. Lane 6 to lane 9, wild-type mice.

References

This product has been referenced in:

  • Compton DR & Martin BR The effect of the enzyme inhibitor phenylmethylsulfonyl fluoride on the pharmacological effect of anandamide in the mouse model of cannabimimetic activity. J Pharmacol Exp Ther 283:1138-43 (1997). Read more (PubMed: 9399986) »
  • Irie T  et al. Evaluation of phenylmethanesulfonyl fluoride (PMSF) as a tracer candidate mapping acetylcholinesterase in vivo. Nucl Med Biol 20:991-2 (1993). Read more (PubMed: 8298579) »
  • Turini P  et al. The action of phenylmethylsulfonyl fluoride on human acetylcholinesterase, chymotyrpsin and trypsin. J Pharmacol Exp Ther 167:98-104 (1969). Read more (PubMed: 4306345) »
See 1 Publication for this product

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