Key features and details
- Rabbit polyclonal to PN-I
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-PN-I antibody
See all PN-I primary antibodies
DescriptionRabbit polyclonal to PN-I
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Chicken, Zebrafish
- Human stomach tissue; HEK lysate; SH-SY5Y cells.
Previously labelled as NT5C3.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab204800 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 38 kDa.|
FunctionCan act both as nucleotidase and as phosphotransferase.
Tissue specificityIsoform 1 and isoform 3 are expressed in reticulocytes and lymphocytes. Isoform 4 is expressed only in reticulocytes.
Involvement in diseaseP5N deficiency
Sequence similaritiesBelongs to the pyrimidine 5'-nucleotidase family.
Cellular localizationCytoplasm and Endoplasmic reticulum.
- Information by UniProt
- 5NT3A_HUMAN antibody
- cN III antibody
- cN-III antibody
All lanes : Anti-PN-I antibody (ab204800) at 1/100 dilution
Lane 1 : Negative Control
Lane 2 : HEK lysate
Lane 2 : HRP-conjugated Swine Anti-Rabbit antibody at 1/3000 dilution
Predicted band size: 38 kDa
Immunohistochemical analysis of formalin-fixed paraffin-embedded Human stomach tissue labeling PN-I with ab204800 at 1/50.
ab204800 has not yet been referenced specifically in any publications.