Anti-PNAD antibody (ab111710)
Key features and details
- Rabbit polyclonal to PNAD
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-PNAD antibody -
Description
Rabbit polyclonal to PNAD -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Pig -
Immunogen
Recombinant fragment corresponding to Human PNAD aa 1-246.
Database link: AAH17336 -
Positive control
- 293T and Raji whole cell lysates; Human hepatoma tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab111710 is purified by antigen-affinity chromatography. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab111710 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/3000. Predicted molecular weight: 35 kDa.
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IHC-P |
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. or Tris-EDTA buffer ( PH8.0)
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 35 kDa. |
IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. or Tris-EDTA buffer ( PH8.0) |
Target
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Relevance
PNAD is required for the ubiquitin-dependent turnover of intracellular proteins that initiate with Met-Asn. This enzyme does not act on substrates with internal or C-terminal asparagines and does not act on glutamine residues in any position. -
Cellular localization
Cytoplasmic -
Database links
- Entrez Gene: 123803 Human
- Entrez Gene: 18203 Mouse
- Entrez Gene: 397107 Pig
- Entrez Gene: 360462 Rat
- SwissProt: Q96AB6 Human
- SwissProt: Q64311 Mouse
- SwissProt: Q28955 Pig
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Alternative names
- N terminal Asn amidase antibody
- N terminal asparagine amidase antibody
- N terminal asparagine amidohydrolase antibody
see all
Images
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab111710 has not yet been referenced specifically in any publications.