Overview

  • Product name

    Anti-PNCK antibody
  • Description

    Rabbit polyclonal to PNCK
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment corresponding to Human PNCK aa 1-115.
    Database link: Q6P2M8

  • Positive control

    • WB: HeLa, MCF7, Jurkat, Raji, U-87 MG and HepG2 whole cell lysate (ab7900); Rat liver tissue lysate; Mouse brain and kidney tissue lysate. IHC-P: Human appendix tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab235093 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/5000.
IHC-P 1/200 - 1/500.

Target

  • Function

    Calcium/calmodulin-dependent protein kinase belonging to a proposed calcium-triggered signaling cascade. In vitro phosphorylates CREB1 and SYN1/synapsin I. Phosphorylates and activates CAMK1.
  • Sequence similarities

    Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications

    Phosphorylated by CAMKK1.
  • Cellular localization

    Cytoplasm. Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • Calcium/calmodulin dependent protein kinase type 1B antibody
    • Calcium/calmodulin-dependent protein kinase type 1B antibody
    • CaM KI beta antibody
    • CaM kinase I beta antibody
    • CaM kinase IB antibody
    • CaM-KI beta antibody
    • CaMK1b antibody
    • CaMKI beta antibody
    • CaMKI-beta antibody
    • EC 2.7.11.17 antibody
    • KCC1B_HUMAN antibody
    • MGC45419 antibody
    • PNCK antibody
    • Pregnancy up regulated non ubiquitously expressed CaM kinase antibody
    • Pregnancy up-regulated non-ubiquitously-expressed CaM kinase antibody
    see all

Images

  • Paraffin-embedded human appendix tissue stained for PNCK using ab235093 at 1/100 dilution in immunohistochemical analysis.

    After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

  • All lanes : Anti-PNCK antibody (ab235093) at 1/500 dilution

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 2 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate
    Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
    Lane 4 : Raji (human Burkitt's lymphoma cell line) whole cell lysate
    Lane 5 : U87-MG (human glioblastoma-astrocytoma epithelial cell line) whole cell lysate
    Lane 6 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
    Lane 7 : Rat liver tissue lysate
    Lane 8 : Mouse brain tissue lysate
    Lane 9 : Mouse kidney tissue lysate

    Secondary
    All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution

References

ab235093 has not yet been referenced specifically in any publications.

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