Overview

  • Product name

    Anti-Pneumolysin antibody [PLY-4]
    See all Pneumolysin primary antibodies
  • Description

    Mouse monoclonal [PLY-4] to Pneumolysin
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, ICC/IF, Neutralisingmore details
  • Species reactivity

    Reacts with: Species independent
  • Immunogen

    Recombinant full length protein

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    pH: 7.50
    Constituent: 0.06% Tris buffered saline
  • Concentration information loading...
  • Purity

    Protein G purified
  • Clonality

    Monoclonal
  • Clone number

    PLY-4
  • Isotype

    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab71810 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 3 µg/ml.
ICC/IF Use at an assay dependent concentration. PubMed: 29636428
Neutralising Use at an assay dependent concentration. PubMed: 8550195

Target

  • Cellular localization

    Plasma membrane

Images

  • Coomassie Blue-stained gel (lane 1) and Western blots of purified recombinant PLY, 3 µg per lane, with mouse monoclonal antibodies PLY-4 (lane 2), PLY-5 (lane 3) and PLY-7 (lane 4); a PLY-irrelevant mouse monoclonal antibody 1.4G8.66 served as control (lane 5).

References

This product has been referenced in:

  • Codemo M  et al. Immunomodulatory Effects of Pneumococcal Extracellular Vesicles on Cellular and Humoral Host Defenses. MBio 9:N/A (2018). WB, ICC/IF . Read more (PubMed: 29636428) »
  • Maurer J  et al. Missing elimination via membrane vesicle shedding contributes to the diminished calcium sensitivity of listeriolysin O. Sci Rep 8:15846 (2018). Read more (PubMed: 30367146) »
See all 10 Publications for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Application
Western blot
Sample
Streptococcus pneumoniae Cell lysate - whole cell (Streptococcus pneumoniae: serotype 3, strain A66)
Gel Running Conditions
Reduced Denaturing (12%)
Loading amount
20 µg
Specification
Streptococcus pneumoniae: serotype 3, strain A66
Blocking step
Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

Abcam user community

Verified customer

Submitted Dec 01 2017

Question
Answer

Gracias por habernos contactado.
Le confirmo la información que me proporcionan mis compañeros del laboratorio: para inmunizar los ratones con los que se produjo ab71810 se utilizó PLY entera.
No dudes en contactarnos para cualquier otra consulta.

Read More

Question
Answer

Thank you for your call today and for letting us know about the trouble with this antibody.

As we discussed, I'm sending two free of charge vials of ab71810 on the order ***, which should arrive tomorrow. I've requested that these vials be inspected for precipitate beforebeing sent out.

Please keep me updated about these new vials and let me know if there is anything else that we can do for you.

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