Product nameAnti-PNGase antibody
See all PNGase primary antibodies
DescriptionRabbit polyclonal to PNGase
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Synthetic peptide within Human PNGase aa 250-300. The exact sequence is proprietary.
Database link: Q96IV0
- WB: HEK-293T and Jurkat whole cell lysate. IP: PNGase IP in HEK-293T whole cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab241350 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000.|
|IP||Use at 2-10 µg/mg of lysate.|
RelevanceNGLY1 is a highly conserved enzyme that catalyzes deglycosylation of misfolded N-linked glycoproteins by cleaving the glycan chain before the proteins are degraded by the proteasome (Zhou et al., 2006).
- CDG1V antibody
- hPNGase antibody
- N glycanase 1 antibody
All lanes : Anti-PNGase antibody (ab241350) at 0.1 µg/ml
Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
Lysates/proteins at 15 µg per lane.
Exposure time: 3 minutes
Prepared using NETN lysis buffer.
PNGase was immunoprecipitated from HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) prepared using NETN lysis buffer.
ab241350 used for IP at 6 µg per reaction. For WB 0.4 µg/ml.
Lane 1: ab241350 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG.
Chemiluminescence detection: 30 seconds.
ab241350 has not yet been referenced specifically in any publications.