Overview

  • Product name

  • Description

    Rabbit polyclonal to PNGase
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human PNGase aa 250-300. The exact sequence is proprietary.
    Database link: Q96IV0

  • Positive control

    • WB: HEK-293T and Jurkat whole cell lysate. IP: PNGase IP in HEK-293T whole cell lysate.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituent: Tris citrate/phosphate

    pH 7 to 8
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab241350 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000.
IP Use at 2-10 µg/mg of lysate.

Target

  • Relevance

    NGLY1 is a highly conserved enzyme that catalyzes deglycosylation of misfolded N-linked glycoproteins by cleaving the glycan chain before the proteins are degraded by the proteasome (Zhou et al., 2006).
  • Cellular localization

    Cytoplasm
  • Database links

  • Alternative names

    • CDG1V antibody
    • hPNGase antibody
    • N glycanase 1 antibody
    • NGLY 1 antibody
    • Peptide N(4) (N acetyl beta glucosaminyl asparagine amidase antibody
    • Peptide:N glycanase antibody
    • PNG1 antibody
    • PNGase antibody
    see all

Images

  • All lanes : Anti-PNGase antibody (ab241350) at 0.1 µg/ml

    Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 2 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
    Lane 3 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate

    Lysates/proteins at 15 µg per lane.

    Exposure time: 3 minutes


    Prepared using NETN lysis buffer.

  • PNGase was immunoprecipitated from HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) prepared using NETN lysis buffer.
    ab241350 used for IP at 6 µg per reaction. For WB 0.4 µg/ml.

    Lane 1: ab241350 IP in HEK-293T whole cell lysate.
    Lane 2: Control IgG.

    Chemiluminescence detection: 30 seconds.

References

ab241350 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab241350.
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