This antibody detects a band corresponding to PNK in an EM9 cell (hamster) lysate overexpressing PNK, it does not detect this band in a wildtype EM9 cell lysate. It detects a band of the same size in Hela cell lysate.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000. Detects a band of approximately 57 kDa (predicted molecular weight: 57 kDa).
Catalyzes the phosphorylation of DNA at 5'-hydroxyl termini and can dephosphorylate its 3'-phosphate termini. Plays an important function in DNA repair following ionizing radiation or oxidative damage.
Expressed in many tissues with highest expression in spleen and testis, and lowest expression in small intestine (PubMed:10446192). Expressed in higher amount in pancreas, heart and kidney and at lower levels in brain, lung and liver (PubMed:10446193).
Involvement in disease
Defects in PNKP are the cause of microcephaly seizures and development delay (MCSZ) [MIM:613402]. It is characterized by microcephaly, infantile-onset seizures, severe intellectual disability and delayed motor milestones with absent speech or only achieving a few words. Most patients also have behavioral problems with hyperactivity. Microcephaly is progressive and without neuronal migration or structural abnormalities, consistent with primary microcephaly.
In the N-terminal section; belongs to the DNA 3' phosphatase family.
Phosphorylated upon DNA damage, probably by ATM or ATR.
Homo sapiens polynucleotide kinase 3' phosphatase (PNKP) antibody
PNK 1 antibody
PNKP DNA kinase antibody
Polynucleotide 3'-phosphatase antibody
Polynucleotide 5' hydroxyl kinase antibody
Polynucleotide 5''-hydroxyl-kinase antibody
Polynucleotide kinase 3 prime phosphatase antibody
Polynucleotide kinase 3' phosphatase antibody
Polynucleotide Kinase antibody
Polynucleotide kinase-3''-phosphatase antibody
Western blot - Anti-PNK antibody (ab4191)
Lane 1: Y190 yeast cell lysate, 10 µg. Lane 2: Y190 yeast cell lysate + human PNK (Gal DNA BP), 10 µg. Lane 3: EM9 XH chinese hamster ovary cell lysate, 10 µg. Lane 4: EM9 XH chinese hamster ovary cell lysate + human PNK, 10 µg. Lane 5: HeLa cell lysate, 10 µg.
ab4191 was incubated for 1-2 hours in TBST at a dilution of 1/1000.