Overview

  • Product name

  • Description

    Rabbit polyclonal to PNK/PNKP
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human PNK/PNKP aa 78-521.
    Database link: Q96T60

  • Positive control

    • A549, HeLa and HepG2 cell lysates; A549 xenograft tissue
  • General notes

     This product was previously labelled as PNK

     

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab151418 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 57 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Catalyzes the phosphorylation of DNA at 5'-hydroxyl termini and can dephosphorylate its 3'-phosphate termini. Plays an important function in DNA repair following ionizing radiation or oxidative damage.
  • Tissue specificity

    Expressed in many tissues with highest expression in spleen and testis, and lowest expression in small intestine (PubMed:10446192). Expressed in higher amount in pancreas, heart and kidney and at lower levels in brain, lung and liver (PubMed:10446193).
  • Involvement in disease

    Defects in PNKP are the cause of microcephaly seizures and development delay (MCSZ) [MIM:613402]. It is characterized by microcephaly, infantile-onset seizures, severe intellectual disability and delayed motor milestones with absent speech or only achieving a few words. Most patients also have behavioral problems with hyperactivity. Microcephaly is progressive and without neuronal migration or structural abnormalities, consistent with primary microcephaly.
  • Sequence similarities

    In the N-terminal section; belongs to the DNA 3' phosphatase family.
  • Post-translational
    modifications

    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • 2''(3'')-polynucleotidase antibody
    • 2'(3')-polynucleotidase antibody
    • Bifunctional polynucleotide phosphatase/kinase antibody
    • DEM 1 antibody
    • DEM1 antibody
    • DNA 5' kinase/3' phosphatase antibody
    • DNA 5''-kinase/3''-phosphatase antibody
    • EIEE10 antibody
    • Homo sapiens polynucleotide kinase 3' phosphatase (PNKP) antibody
    • MCSZ antibody
    • PNK 1 antibody
    • PNK1 antibody
    • Pnkp antibody
    • PNKP DNA kinase antibody
    • PNKP_HUMAN antibody
    • Polynucleotide 3'-phosphatase antibody
    • Polynucleotide 5' hydroxyl kinase antibody
    • Polynucleotide 5''-hydroxyl-kinase antibody
    • Polynucleotide kinase 3 prime phosphatase antibody
    • Polynucleotide kinase 3' phosphatase antibody
    • Polynucleotide Kinase antibody
    • Polynucleotide kinase-3''-phosphatase antibody
    see all

Images

  • Anti-PNK/PNKP antibody (ab151418) at 1/2000 dilution + A549 whole cell lysate at 30 µg

    Predicted band size: 57 kDa



    7.5% SDS PAGE
  • Immunohistochemical analysis of paraffin-embedded A549 xenograft labeling PNK/PNKP with ab151418 at 1/500 dilution.

References

This product has been referenced in:

  • Prati B  et al. Three Prime Repair Exonuclease 1 (TREX1) expression correlates with cervical cancer cells growth in vitro and disease progression in vivo. Sci Rep 9:351 (2019). Read more (PubMed: 30674977) »
See 1 Publication for this product

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